Q4JAP9 · LYSX_SULAC

Function

function

Catalyzes the ATP-dependent formation of a covalent bond between the amino group of alpha-aminoadipate (AAA) and the gamma-carboxyl group of the C-terminal glutamate residue in LysW.

Catalytic activity

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 2 magnesium ions per subunit.

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via AAA pathway; L-lysine from L-alpha-aminoadipate (Thermus route): step 1/5.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site82ATP (UniProtKB | ChEBI)
Binding site122ATP (UniProtKB | ChEBI)
Binding site126-132ATP (UniProtKB | ChEBI)
Binding site162-173ATP (UniProtKB | ChEBI)
Binding site187ATP (UniProtKB | ChEBI)
Binding site196ATP (UniProtKB | ChEBI)
Binding site231Mg2+ 1 (UniProtKB | ChEBI)
Binding site244Mg2+ 1 (UniProtKB | ChEBI)
Binding site244Mg2+ 2 (UniProtKB | ChEBI)
Binding site246Mg2+ 2 (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular Functionligase activity
Molecular Functionmetal ion binding
Biological Processlysine biosynthetic process via aminoadipic acid
Biological Processprotein modification process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Alpha-aminoadipate--LysW ligase LysX
  • EC number
  • Short names
    AAA--LysW ligase LysX

Gene names

    • Name
      lysX
    • Ordered locus names
      Saci_0754

Organism names

Accessions

  • Primary accession
    Q4JAP9

Proteomes

Phenotypes & Variants

Disruption phenotype

Cells lacking this gene require lysine for growth.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis253-254Alters substrate specificity, so that glutamate is preferred over alpha-aminoadipate.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004229921-276Alpha-aminoadipate--LysW ligase LysX

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain, motif.

TypeIDPosition(s)Description
Domain86-271ATP-grasp
Motif253-254N-[TS] motif that is essential for LysX substrate specificity

Sequence similarities

Belongs to the RimK family. LysX subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    276
  • Mass (Da)
    31,712
  • Last updated
    2005-08-02 v1
  • Checksum
    3BA4CE0980E8B635
MRWEEKDIITEAKKSGFKAIPIFTKDFYSAIGVGENYSELEADVIIQRNTSHARALTTSLIFEGWNYNVVNDATSLFKCGNKLYTLSLLAKHNIKTPRTIVTFSKDKAVDLAKKIGFPAVIKPIEGSWGRMVAKAVDEDILYSFLEYQEYTTSQFRQIYLVQEFVKKPNRDIRIFVMGDEAPVGIYRVNERNWKTNTALGARALPLKIDDELRDLALKVRDIMGGFFLGIDIFEDPERGYLVNEVNGVPEYKNTVRVNNFNVSSYLLNKLREWIKK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000077
EMBL· GenBank· DDBJ
AAY80130.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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