Q4I8X0 · TRM10_GIBZE
- ProteintRNA (guanine(9)-N1)-methyltransferase
- GeneTRM10
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids407 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
S-adenosyl-L-methionine-dependent guanine N1-methyltransferase that catalyzes the formation of N1-methylguanine at position 9 (m1G9) in cytoplasmic tRNA.
Catalytic activity
- guanosine9 in tRNA + S-adenosyl-L-methionine = H+ + N1-methylguanosine9 in tRNA + S-adenosyl-L-homocysteine
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 263-264 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: LS | ||||||
Binding site | 283 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Active site | 287 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 287-291 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: DKNRE | ||||||
Binding site | 295 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: C | ||||||
Binding site | 309 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: L | ||||||
Binding site | 321-323 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | ||||
Sequence: TVL |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | nucleus | |
Molecular Function | tRNA (guanosine(9)-N1)-methyltransferase activity | |
Molecular Function | tRNA binding | |
Biological Process | tRNA N1-guanine methylation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA (guanine(9)-N1)-methyltransferase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Sordariomycetes > Hypocreomycetidae > Hypocreales > Nectriaceae > Fusarium
Accessions
- Primary accessionQ4I8X0
- Secondary accessions
Proteomes
Organism-specific databases
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000060516 | 1-407 | tRNA (guanine9-N1)-methyltransferase | |||
Sequence: MDLDPAHKPSQAEETKEQGNEQGQVEQNQAQQDPTTGPQAETEKAIIPSQGSTIPQKRSAEDEPAQPMSKNALKRLRKQQQWEAGKEDRKLKRKDSRIARKVRKREERDALIAQGINPYANKQKPPSVNVPISLIFDCEFEQYMREKEIISLGSQITRSYSENKNAKYRTNIYVSNWNGKLAERFHQILDDKHQNWKGIDFVEGDFIECAEKAREKMKHENMIEPLQRSLTEKSPWARDEKDPLPLPDPEPEPRPEYSDIVYLSSDSPYTLERLEPNTSYVIGGLVDKNREKGLCYKRARERGIRTARLPIGQYMVMQSRTVLTTNHVVEIMLKWLEYENWGEAFMSVIPKRKGGKLKEQQGASGETQETEEAEAEDPEEENEETKDPDAEASASKQNTPKVEVTSK |
Interaction
Structure
Family & Domains
Features
Showing features for compositional bias, region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-15 | Basic and acidic residues | ||||
Sequence: MDLDPAHKPSQAEET | ||||||
Region | 1-105 | Disordered | ||||
Sequence: MDLDPAHKPSQAEETKEQGNEQGQVEQNQAQQDPTTGPQAETEKAIIPSQGSTIPQKRSAEDEPAQPMSKNALKRLRKQQQWEAGKEDRKLKRKDSRIARKVRKR | ||||||
Compositional bias | 16-59 | Polar residues | ||||
Sequence: KEQGNEQGQVEQNQAQQDPTTGPQAETEKAIIPSQGSTIPQKRS | ||||||
Compositional bias | 76-105 | Basic and acidic residues | ||||
Sequence: LRKQQQWEAGKEDRKLKRKDSRIARKVRKR | ||||||
Domain | 120-356 | SAM-dependent MTase TRM10-type | ||||
Sequence: ANKQKPPSVNVPISLIFDCEFEQYMREKEIISLGSQITRSYSENKNAKYRTNIYVSNWNGKLAERFHQILDDKHQNWKGIDFVEGDFIECAEKAREKMKHENMIEPLQRSLTEKSPWARDEKDPLPLPDPEPEPRPEYSDIVYLSSDSPYTLERLEPNTSYVIGGLVDKNREKGLCYKRARERGIRTARLPIGQYMVMQSRTVLTTNHVVEIMLKWLEYENWGEAFMSVIPKRKGGK | ||||||
Region | 220-256 | Disordered | ||||
Sequence: ENMIEPLQRSLTEKSPWARDEKDPLPLPDPEPEPRPE | ||||||
Compositional bias | 233-255 | Basic and acidic residues | ||||
Sequence: KSPWARDEKDPLPLPDPEPEPRP | ||||||
Region | 353-407 | Disordered | ||||
Sequence: KGGKLKEQQGASGETQETEEAEAEDPEEENEETKDPDAEASASKQNTPKVEVTSK | ||||||
Compositional bias | 369-387 | Acidic residues | ||||
Sequence: ETEEAEAEDPEEENEETKD | ||||||
Compositional bias | 392-407 | Polar residues | ||||
Sequence: ASASKQNTPKVEVTSK |
Sequence similarities
Belongs to the class IV-like SAM-binding methyltransferase superfamily. TRM10 family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length407
- Mass (Da)46,769
- Last updated2005-08-16 v1
- Checksum30151BC819DC5C24
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 1-15 | Basic and acidic residues | ||||
Sequence: MDLDPAHKPSQAEET | ||||||
Compositional bias | 16-59 | Polar residues | ||||
Sequence: KEQGNEQGQVEQNQAQQDPTTGPQAETEKAIIPSQGSTIPQKRS | ||||||
Compositional bias | 76-105 | Basic and acidic residues | ||||
Sequence: LRKQQQWEAGKEDRKLKRKDSRIARKVRKR | ||||||
Compositional bias | 233-255 | Basic and acidic residues | ||||
Sequence: KSPWARDEKDPLPLPDPEPEPRP | ||||||
Compositional bias | 369-387 | Acidic residues | ||||
Sequence: ETEEAEAEDPEEENEETKD | ||||||
Compositional bias | 392-407 | Polar residues | ||||
Sequence: ASASKQNTPKVEVTSK |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
DS231665 EMBL· GenBank· DDBJ | ESU12419.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
HG970334 EMBL· GenBank· DDBJ | CEF87515.1 EMBL· GenBank· DDBJ | Genomic DNA |