Q4H4E3 · BTRV_NIACI

Function

function

NAD(P)H:FMN oxidoreductase component of a two-component system involved in the biosynthesis of the side chain of the aminoglycoside antibiotics in the biosynthetic pathway of butirosin. Together with BtrO, mediates hydroxylation of gamma-L-Glu-GABA-S-BtrI.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
6.86 μMNADH in the presence of FMN
7.25 μMNADH in the presence of FAD
kcat is 1243.13 min-1 with NADH as substrate in the presence of FMN. kcat is 887.48 min-1 with NADH as substrate in the presence of FAD.

Pathway

Antibiotic biosynthesis; butirosin biosynthesis.

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    4-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein] monooxygenase BtrO
  • EC number
  • Alternative names
    • Butirosin biosynthesis protein V

Gene names

    • Name
      btrV

Organism names

Accessions

  • Primary accession
    Q4H4E3

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004217281-824-(gamma-L-glutamylamino)butanoyl-[BtrI acyl-carrier protein] monooxygenase BtrO

Interaction

Subunit

Homotetramer.

Structure

Family & Domains

Sequence

  • Sequence status
    Complete
  • Length
    82
  • Mass (Da)
    9,416
  • Last updated
    2005-08-30 v1
  • Checksum
    6456EA01819A7269
MDNKERNTLYQVVYAISGVTGEDGDELVETFKQGPMEVDKRDFFEIVQRVESLFDCTLDMNLEGPYLIHADEIVTKITKLNV

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AB097196
EMBL· GenBank· DDBJ
BAE07078.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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