Q4DER6 · DRE22_TRYCC

Function

function

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for the maturation of extramitochondrial Fe-S proteins. Part of an electron transfer chain functioning in an early step of cytosolic Fe-S biogenesis, facilitating the de novo assembly of a [4Fe-4S] cluster on the cytosolic Fe-S scaffold complex. Electrons are transferred from NADPH via a FAD- and FMN-containing diflavin oxidoreductase. Together with the diflavin oxidoreductase, also required for the assembly of the diferric tyrosyl radical cofactor of ribonucleotide reductase (RNR), probably by providing electrons for reduction during radical cofactor maturation in the catalytic small subunit.

Cofactor

Protein has several cofactor binding sites:
[2Fe-2S] cluster (UniProtKB | Rhea| CHEBI:190135 )

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Features

Showing features for binding site.

1115102030405060708090100110
TypeIDPosition(s)Description
Binding site39[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site46[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site49[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site51[2Fe-2S] cluster (UniProtKB | ChEBI)
Binding site77[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site80[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site88[4Fe-4S] cluster (UniProtKB | ChEBI)
Binding site91[4Fe-4S] cluster (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentmitochondrial intermembrane space
Molecular Function2 iron, 2 sulfur cluster binding
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Biological Processiron-sulfur cluster assembly

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Anamorsin homolog 2
  • Alternative names
    • Fe-S cluster assembly protein DRE2 homolog 2

Gene names

    • ORF names
      Tc00.1047053505071.119

Organism names

  • Taxonomic identifier
  • Strain
    • CL Brener
  • Taxonomic lineage
    Eukaryota > Discoba > Euglenozoa > Kinetoplastea > Metakinetoplastina > Trypanosomatida > Trypanosomatidae > Trypanosoma > Schizotrypanum

Accessions

  • Primary accession
    Q4DER6

Proteomes

Subcellular Location

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003923691-115Anamorsin homolog 2

Proteomic databases

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, motif.

Type
IDPosition(s)Description
Region30-115Disordered
Region39-51Fe-S binding site A
Motif77-80Cx2C motif 1
Region77-91Fe-S binding site B
Motif88-91Cx2C motif 2

Domain

The C-terminal domain binds 2 Fe-S clusters but is otherwise mostly in an intrinsically disordered conformation.
The N-terminal domain has structural similarity with S-adenosyl-L-methionine-dependent methyltransferases, but does not bind S-adenosyl-L-methionine. It is required for correct assembly of the 2 Fe-S clusters.
The twin Cx2C motifs are involved in the recognition by the mitochondrial MIA40-ERV1 disulfide relay system. The formation of 2 disulfide bonds in the Cx2C motifs through dithiol/disulfide exchange reactions effectively traps the protein in the mitochondrial intermembrane space.

Sequence similarities

Belongs to the anamorsin family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    115
  • Mass (Da)
    12,508
  • Last updated
    2005-09-13 v1
  • Checksum
    61C0C2C03F568F65
MSSKPTQAFSLKTRQAVDEDALLTEEDRVVKEATKGEDCTTRRRACKNCTCGRAELERKMLAEGKKVEMPQMPAGGCGNCAKGDAFRCATCPFLGQPAFDNTSDGKVKLNLTDDI

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AAHK01000563
EMBL· GenBank· DDBJ
EAN91028.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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