Q4DA80 · PRCMA_TRYCC

Function

function

Catalyzes the interconversion of L- and D-proline. Secreted isoform 1 contributes to parasite immune evasion by acting as a B-cell mitogen. Probably involved in parasite differentiation and infectivity.

Catalytic activity

Activity regulation

Inhibited by maleic acid, iodoacetamide, iodoacetate and, most particularly, pyrrole-2-carboxylic acid.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
29 mML-proline
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
0.053 mmol/sec/ug

pH Dependence

Optimum pH is 5.5-7.0.

Features

Showing features for active site, binding site.

TypeIDPosition(s)Description
Active site160Proton acceptor
Binding site161-162substrate
Binding site326substrate
Active site330Proton donor
Binding site331-332substrate

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentextracellular region
Cellular Componentmembrane
Molecular Function4-hydroxyproline epimerase activity
Molecular Functionproline racemase activity

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Proline racemase A
  • EC number
  • Alternative names
    • TcPA45-A
    • TcPRACA
    • rTcPA45

Gene names

    • Name
      PA45-A
    • ORF names
      Tc00.1047053506795.80

Organism names

  • Taxonomic identifier
  • Strain
    • CL Brener
  • Taxonomic lineage
    Eukaryota > Discoba > Euglenozoa > Kinetoplastea > Metakinetoplastina > Trypanosomatida > Trypanosomatidae > Trypanosoma > Schizotrypanum

Accessions

  • Primary accession
    Q4DA80
  • Secondary accessions
    • Q9NCP4

Proteomes

Subcellular Location

Isoform 1

Secreted
Membrane
Note: Membrane-bound and secreted upon differentiation of the parasite into non-dividing infective forms, suggesting that isoform 1 appears upon differentiation (PubMed:16164548).

Isoform 2

Cytoplasm
Note: Cytoplasmic in replicative non-infective forms (PubMed:16164548).

Keywords

Phenotypes & Variants

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis160Loss of enzyme activity, without abrogating mitogenic properties.
Mutagenesis330Loss of enzyme activity, without abrogating mitogenic properties.

PTM/Processing

Features

Showing features for signal, chain, glycosylation.

TypeIDPosition(s)Description
Signal1-31
ChainPRO_500005722532-423Proline racemase A
Glycosylation213N-linked (GlcNAc...) asparagine
Glycosylation266N-linked (GlcNAc...) asparagine
Glycosylation282N-linked (GlcNAc...) asparagine

Keywords

Proteomic databases

PTM databases

Interaction

Subunit

Homodimer.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the proline racemase family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.

This entry describes 2 isoforms produced by Alternative splicing.

Q4DA80-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    423
  • Mass (Da)
    46,950
  • Last updated
    2007-05-29 v2
  • Checksum
    2BF6DFF8F5AB662E
MRKSVCPKQKFFFSAFPFFFFFCVFPLISRTGQEKLLFDQKYKIIKGEKKEKKKNQRANRREHQQKREIMRFKKSFTCIDMHTEGEAARIVTSGLPHIPGSNMAEKKAYLQENMDYLRRGIMLEPRGHDDMFGAFLFDPIEEGADLGMVFMDTGGYLNMCGHNSIAAVTAAVETGIVSVPAKATNVPVVLDTPAGLVRGTAHLQSGTESEVSNASIINVPSFLYQQDVVVVLPKPYGEVRVDIAFGGNFFAIVPAEQLGIDISVQNLSRLQEAGELLRTEINRSVKVQHPQLPHINTVDCVEIYGPPTNPEANYKNVVIFGNRQADRSPCGTGTSAKMATLYAKGQLRIGETFVYESILGSLFQGRVLGEERIPGVKVPVTKDAEEGMLVVTAEITGKAFIMGFNTMLFDPTDPFKNGFTLKQ

Q4DA80-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Sequence caution

The sequence EAN89436.1 differs from that shown. Reason: Erroneous gene model prediction

Features

Showing features for alternative sequence, sequence conflict.

TypeIDPosition(s)Description
Alternative sequenceVSP_0258491-69in isoform 2
Sequence conflict79in Ref. 1; AAF97423

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AF195522
EMBL· GenBank· DDBJ
AAF97423.1
EMBL· GenBank· DDBJ
Genomic DNA
AAHK01000747
EMBL· GenBank· DDBJ
EAN89436.1
EMBL· GenBank· DDBJ
Genomic DNA Sequence problems.

Genome annotation databases

Similar Proteins

Disclaimer

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