Q4DA80 · PRCMA_TRYCC
- ProteinProline racemase A
- GenePA45-A
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids423 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes the interconversion of L- and D-proline. Secreted isoform 1 contributes to parasite immune evasion by acting as a B-cell mitogen. Probably involved in parasite differentiation and infectivity.
Catalytic activity
- L-proline = D-proline
Activity regulation
Inhibited by maleic acid, iodoacetamide, iodoacetate and, most particularly, pyrrole-2-carboxylic acid.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
29 mM | L-proline |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
0.053 mmol/sec/ug |
pH Dependence
Optimum pH is 5.5-7.0.
Features
Showing features for active site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 160 | Proton acceptor | ||||
Sequence: C | ||||||
Binding site | 161-162 | substrate | ||||
Sequence: GH | ||||||
Binding site | 326 | substrate | ||||
Sequence: D | ||||||
Active site | 330 | Proton donor | ||||
Sequence: C | ||||||
Binding site | 331-332 | substrate | ||||
Sequence: GT |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | extracellular region | |
Cellular Component | membrane | |
Molecular Function | 4-hydroxyproline epimerase activity | |
Molecular Function | proline racemase activity |
Keywords
- Molecular function
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProline racemase A
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Discoba > Euglenozoa > Kinetoplastea > Metakinetoplastina > Trypanosomatida > Trypanosomatidae > Trypanosoma > Schizotrypanum
Accessions
- Primary accessionQ4DA80
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 160 | Loss of enzyme activity, without abrogating mitogenic properties. | ||||
Sequence: C → S | ||||||
Mutagenesis | 330 | Loss of enzyme activity, without abrogating mitogenic properties. | ||||
Sequence: C → S |
PTM/Processing
Features
Showing features for signal, chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-31 | |||||
Sequence: MRKSVCPKQKFFFSAFPFFFFFCVFPLISRT | ||||||
Chain | PRO_5000057225 | 32-423 | Proline racemase A | |||
Sequence: GQEKLLFDQKYKIIKGEKKEKKKNQRANRREHQQKREIMRFKKSFTCIDMHTEGEAARIVTSGLPHIPGSNMAEKKAYLQENMDYLRRGIMLEPRGHDDMFGAFLFDPIEEGADLGMVFMDTGGYLNMCGHNSIAAVTAAVETGIVSVPAKATNVPVVLDTPAGLVRGTAHLQSGTESEVSNASIINVPSFLYQQDVVVVLPKPYGEVRVDIAFGGNFFAIVPAEQLGIDISVQNLSRLQEAGELLRTEINRSVKVQHPQLPHINTVDCVEIYGPPTNPEANYKNVVIFGNRQADRSPCGTGTSAKMATLYAKGQLRIGETFVYESILGSLFQGRVLGEERIPGVKVPVTKDAEEGMLVVTAEITGKAFIMGFNTMLFDPTDPFKNGFTLKQ | ||||||
Glycosylation | 213 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 266 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Glycosylation | 282 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Keywords
- PTM
Proteomic databases
PTM databases
Structure
Family & Domains
Sequence similarities
Belongs to the proline racemase family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
This entry describes 2 isoforms produced by Alternative splicing.
Q4DA80-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length423
- Mass (Da)46,950
- Last updated2007-05-29 v2
- Checksum2BF6DFF8F5AB662E
Q4DA80-2
- Name2
- Differences from canonical
- 1-69: Missing
Sequence caution
Features
Showing features for alternative sequence, sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_025849 | 1-69 | in isoform 2 | |||
Sequence: Missing | ||||||
Sequence conflict | 79 | in Ref. 1; AAF97423 | ||||
Sequence: I → M |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF195522 EMBL· GenBank· DDBJ | AAF97423.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AAHK01000747 EMBL· GenBank· DDBJ | EAN89436.1 EMBL· GenBank· DDBJ | Genomic DNA | Sequence problems. |