Q4A7U1 · MNMA_MESH7

Function

function

Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.

Features

Showing features for binding site, active site, site.

137250100150200250300350
Type
IDPosition(s)Description
Binding site7-14ATP (UniProtKB | ChEBI)
Binding site33ATP (UniProtKB | ChEBI)
Active site109Nucleophile
Binding site134ATP (UniProtKB | ChEBI)
Site135Interaction with tRNA
Active site202Cysteine persulfide intermediate
Site342Interaction with tRNA

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular FunctiontRNA binding
Molecular FunctiontRNA-uridine 2-sulfurtransferase activity
Biological ProcesstRNA wobble position uridine thiolation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA-specific 2-thiouridylase MnmA
  • EC number

Gene names

    • Name
      mnmA
    • Synonyms
      trmU
    • Ordered locus names
      MHP7448_0430

Organism names

Accessions

  • Primary accession
    Q4A7U1

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain, disulfide bond.

Type
IDPosition(s)Description
ChainPRO_10000095361-372tRNA-specific 2-thiouridylase MnmA
Disulfide bond109↔202Alternate

Keywords

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region104-106Interaction with target base in tRNA
Region152-154Interaction with tRNA
Region310-311Interaction with tRNA

Sequence similarities

Belongs to the MnmA/TRMU family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    372
  • Mass (Da)
    42,403
  • Last updated
    2005-09-13 v1
  • Checksum
    88F6998A1797A7FA
MAKIVVGLSGGVDSAVSAYLLKKAGHNVIAVFMRNWDSSLNNDFLGKKNEKNFTICPQEQDWLDAKVVAKQLNIPIFRIDFIKEYWDEVFSDLILKYQSGLTPNPDILCNKNIKFKHFLDYAQKVHNADFIAMGHYAKTDNGNLYAGADSLKDQSYFLGQLSKSQLQKTIFPLGNLLKSEVRKIANELGLINAKKKDSTGICFIGERKFTDFLQNYIPAQPGNIIDISTKKVLGKHIGIMYFTIGQRKGFGLSGMKEPYFVVGHNLKEKILYVSPQSEKKWLESDSLMAKNANFLSENFRNLDNLSAKFRYRQEAIPIRIEKIQDNSFWISYQKYQAITPGQQVVIYHQNQVILAGEIALLFRNGKKLDYLD

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AE017244
EMBL· GenBank· DDBJ
AAZ53798.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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