Q4A7U1 · MNMA_MESH7
- ProteintRNA-specific 2-thiouridylase MnmA
- GenemnmA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids372 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the 2-thiolation of uridine at the wobble position (U34) of tRNA, leading to the formation of s2U34.
Catalytic activity
- uridine34 in tRNA + S-sulfanyl-L-cysteinyl-[protein] + AH2 + ATP = 2-thiouridine34 in tRNA + L-cysteinyl-[protein] + A + AMP + diphosphate + H+
RHEA-COMP:11727 CHEBI:65315 Position: 34+ RHEA-COMP:11726 + CHEBI:17499 + CHEBI:30616 = RHEA-COMP:11728 CHEBI:87170 Position: 34+ RHEA-COMP:10131 + CHEBI:13193 + CHEBI:456215 + CHEBI:33019 + CHEBI:15378
Features
Showing features for binding site, active site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 7-14 | ATP (UniProtKB | ChEBI) | |||
Binding site | 33 | ATP (UniProtKB | ChEBI) | |||
Active site | 109 | Nucleophile | |||
Binding site | 134 | ATP (UniProtKB | ChEBI) | |||
Site | 135 | Interaction with tRNA | |||
Active site | 202 | Cysteine persulfide intermediate | |||
Site | 342 | Interaction with tRNA | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Molecular Function | ATP binding | |
Molecular Function | tRNA binding | |
Molecular Function | tRNA-uridine 2-sulfurtransferase activity | |
Biological Process | tRNA wobble position uridine thiolation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-specific 2-thiouridylase MnmA
- EC number
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Mycoplasmatota > Mycoplasmoidales > Metamycoplasmataceae > Mesomycoplasma
Accessions
- Primary accessionQ4A7U1
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for chain, disulfide bond.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_1000009536 | 1-372 | tRNA-specific 2-thiouridylase MnmA | ||
Disulfide bond | 109↔202 | Alternate | |||
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 104-106 | Interaction with target base in tRNA | |||
Region | 152-154 | Interaction with tRNA | |||
Region | 310-311 | Interaction with tRNA | |||
Sequence similarities
Belongs to the MnmA/TRMU family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length372
- Mass (Da)42,403
- Last updated2005-09-13 v1
- Checksum88F6998A1797A7FA
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AE017244 EMBL· GenBank· DDBJ | AAZ53798.1 EMBL· GenBank· DDBJ | Genomic DNA |