Q49575 · A85B_MYCIA
- ProteinDiacylglycerol acyltransferase/mycolyltransferase Ag85B
- GenefbpB
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids330 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
The antigen 85 proteins (FbpA, FbpB, FbpC) are responsible for the high affinity of mycobacteria for fibronectin, a large adhesive glycoprotein, which facilitates the attachment of M.tuberculosis to murine alveolar macrophages (AMs). They also help to maintain the integrity of the cell wall by catalyzing the transfer of mycolic acids to cell wall arabinogalactan and through the synthesis of alpha,alpha-trehalose dimycolate (TDM, cord factor). They catalyze the transfer of a mycoloyl residue from one molecule of alpha,alpha-trehalose monomycolate (TMM) to another TMM, leading to the formation of TDM (By similarity).
Catalytic activity
- 2 alpha,alpha'-trehalose 6-mycolate = alpha,alpha'-trehalose 6,6'-bismycolate + alpha,alpha-trehalose
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 82-83 | substrate | ||||
Sequence: LR | ||||||
Active site | 166 | Nucleophile | ||||
Sequence: S | ||||||
Binding site | 166 | substrate | ||||
Sequence: S | ||||||
Binding site | 194 | substrate | ||||
Sequence: D | ||||||
Active site | 270 | |||||
Sequence: E | ||||||
Binding site | 272-275 | substrate | ||||
Sequence: FVRS | ||||||
Binding site | 279 | substrate | ||||
Sequence: K | ||||||
Active site | 302 | |||||
Sequence: H | ||||||
Binding site | 302-304 | substrate | ||||
Sequence: HSW |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | diacylglycerol O-acyltransferase activity | |
Molecular Function | trehalose O-mycolyltransferase activity |
Keywords
- Molecular function
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameDiacylglycerol acyltransferase/mycolyltransferase Ag85B
- EC number
- Short namesDGAT
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Actinomycetota > Actinomycetes > Mycobacteriales > Mycobacteriaceae > Mycobacterium > Mycobacterium avium complex (MAC)
Accessions
- Primary accessionQ49575
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for signal, chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-40 | |||||
Sequence: MTDLSEKVRAWGRRLVVGAAAAATLPGLIGIAGGAATANA | ||||||
Chain | PRO_0000000218 | 41-330 | Diacylglycerol acyltransferase/mycolyltransferase Ag85B | |||
Sequence: FSRPGLPVEYLQVPSAGMGRDIKVQFQSGGNGSPAVYLLDGLRAQDDYNGWDINTPAFEWYYQSGLSVIMPVGGQSSFYADWYQPACGKAGCSTYKWETFLTSELPQYLASNKGVKSTGSAAVGISMSGSSAMILAVNHPNQFVYAGSLSALLDPSQGMGPSLIGLAMGDAGGYKADAMWGPSSDPAWQRNDPSLQIPALVGNNTRLWVYCGNGTPSELGGANMPAEFLENFVRSSNLKFQDAYNAAGGHNAVFNFNANGTHSWEYWGAQLNAMKPDLQSALGASSGGGG | ||||||
Disulfide bond | 127↔132 | |||||
Sequence: CGKAGC |
Keywords
- PTM
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 98-108 | Fibronectin-binding | ||||
Sequence: FEWYYQSGLSV |
Sequence similarities
Belongs to the mycobacterial A85 antigen family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length330
- Mass (Da)34,522
- Last updated2012-06-13 v2
- Checksum76FDB684BF4E50D4
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 47-48 | in Ref. 1; BAA03243 | ||||
Sequence: PV → EF | ||||||
Sequence conflict | 138 | in Ref. 1; BAA03243/BAA03981 | ||||
Sequence: E → D | ||||||
Sequence conflict | 310 | in Ref. 1; BAA03243 | ||||
Sequence: Q → S |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
D16546 EMBL· GenBank· DDBJ | BAA03981.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
D14253 EMBL· GenBank· DDBJ | BAA03243.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP003322 EMBL· GenBank· DDBJ | AFC43854.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation |