Q48722 · LTRB_LACLC

Function

function

Mediates initition of conjugal transfer of plasmid pRS01 possibly by introducing a single-stranded nick at the potential origin of transfer, to initiate single strand transfer from donor to recipient.

Miscellaneous

The gene coding for this protein is interrupted by a group II intron, Ll.LtrB. Splicing of the intervening intron is necessary for proper protein activity and, therefore, conjugative transfer of pRS01, as the splicing event brings together the two conserved histidine residues proposed to function as ligands for the metal ion cofactor.

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for active site, binding site.

156350100150200250300350400450500550
Type
IDPosition(s)Description
Active site44
Binding site159Mg2+ (UniProtKB | ChEBI)
Binding site161Mg2+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionmetal ion binding

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Group II intron-interrupted relaxase LtrB
  • Alternative names
    • Conjugative nickase

Gene names

    • Name
      ltrBE1
    • Name
      ltrBE2

Encoded on

  • Plasmid pRS01

Organism names

Accessions

  • Primary accession
    Q48722

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00000845141-563Group II intron-interrupted relaxase LtrB

Structure

Family & Domains

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    563
  • Mass (Da)
    64,870
  • Last updated
    2007-12-04 v2
  • Checksum
    BB1A204177662CE7
MVYTKHIIVHKLKHLRQAKDYVENAEKTLVNESNEDHLTNLFPYISNPDKTMSKQLVSGHGITNVYDAANEFIATKKLKALSKGTDFNFDPQTGKVRFNVESLEKNNAVLGHHLIQSFSPDDNLTPEQIHEIGRQTILEFTGGEYEFVIATHVDREHIHNHIIFNSTNLYTGKQFDWKVIPKEKTKSGKAYDVTKNNFEKVSDKIASRYGAKIIEKSPGNSHLKYTKWQTQSIYKSQIKQRLDYLLEMSSDIEDFKRKATALNLSFDFSGKWTTYRLLDEPQMKNTRGRNLDKNRPEKYNLESIIERLETNELSLTVDEVVERYEEKVDVVKQDFDYQVTVEKGQIDHMTSKGFYLNVDFGIADRGQIFIGGYKVDQLENRDCVLYLKKNETFRLLSEKEASFTKYLTGHDLAKQLGLYNGTVPLKKEPVISTINQLVDAINFLAEHGVTEGTQFNNMESQLMSALGEAEEKLYVIDNKIMELTKIAKLLIEKESDHSQAVINELENLGVGPSIKYQDIHQELQSEKMSRKILKNKFEQTVDEINTFNEIRVTTLEENKGKIL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U50902
EMBL· GenBank· DDBJ
AAB06502.2
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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