Q47642 · Q47642_ECOLX
- ProteinrRNA N-glycosylase
- Genesubunit A
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids319 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
The A subunit is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits. After endocytosis, the A subunit is cleaved by furin in two fragments, A1 and A2: A1 is the catalytically active fragment, and A2 is essential for holotoxin assembly with the B subunits.
Catalytic activity
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 189 | |||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | rRNA N-glycosylase activity | |
Molecular Function | toxin activity | |
Biological Process | negative regulation of translation |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended namerRNA N-glycosylase
- EC number
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionQ47642
PTM/Processing
Features
Showing features for signal, chain, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-22 | |||||
Sequence: MKCILFKWVLCLLLGFSSVSYS | ||||||
Chain | PRO_5004233137 | 23-319 | rRNA N-glycosylase | |||
Sequence: REFTIDFSTQQSYVSSLNSIRTEVSTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAGFVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSSYLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDVDLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNEESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQSLYTTGE | ||||||
Disulfide bond | 263↔282 | |||||
Sequence: CHHQGARSVRAVNEESQPEC |
Keywords
- PTM
Structure
Sequence
- Sequence statusComplete
- Length319
- Mass (Da)35,641
- Last updated1996-11-01 v1
- Checksum90D2E7F23CA6C999