Q47642 · Q47642_ECOLX

  • Protein
    rRNA N-glycosylase
  • Gene
    subunit A
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Inferred from homology
  • Annotation score
    2/5

Function

function

The A subunit is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits. After endocytosis, the A subunit is cleaved by furin in two fragments, A1 and A2: A1 is the catalytically active fragment, and A2 is essential for holotoxin assembly with the B subunits.

Catalytic activity

  • Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
    EC:3.2.2.22 (UniProtKB | ENZYME | Rhea)

Features

Showing features for active site.

131950100150200250300
TypeIDPosition(s)Description
Active site189

GO annotations

AspectTerm
Molecular FunctionrRNA N-glycosylase activity
Molecular Functiontoxin activity
Biological Processnegative regulation of translation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    rRNA N-glycosylase
  • EC number

Gene names

    • Name
      subunit A

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • 7279
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia

Accessions

  • Primary accession
    Q47642

PTM/Processing

Features

Showing features for signal, chain, disulfide bond.

TypeIDPosition(s)Description
Signal1-22
ChainPRO_500423313723-319rRNA N-glycosylase
Disulfide bond263↔282

Keywords

Family & Domains

Sequence similarities

Belongs to the ribosome-inactivating protein family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    319
  • Mass (Da)
    35,641
  • Last updated
    1996-11-01 v1
  • Checksum
    90D2E7F23CA6C999
MKCILFKWVLCLLLGFSSVSYSREFTIDFSTQQSYVSSLNSIRTEVSTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAGFVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSSYLALMEFSGNTMTRDASRAVLRFVTVTAEALRFRQIQREFRQALSETAPVYTMTPGDVDLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNEESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQSLYTTGE

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
X61283
EMBL· GenBank· DDBJ
CAA43585.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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