Q46821 · UACT_ECOLI
- ProteinUric acid transporter UacT
- GeneuacT
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids482 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Proton-dependent high-capacity transporter for uric acid. Shows also a low capacity for transport of xanthine at 37 degrees Celsius but not at 25 degrees Celsius.
Activity regulation
Inhibited in the presence of the protonophore carbonyl cyanide m-chlorophenyl hydrazone.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.5 mM | uric acid | |||||
0.3 mM | xanthine |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
715 nmol/min/mg | with acid uric as substrate | ||||
14 nmol/min/mg | 37 | with xanthine as substrate |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | symporter activity | |
Molecular Function | urate transmembrane transporter activity | |
Molecular Function | xanthine transmembrane transporter activity | |
Biological Process | proton transmembrane transport | |
Biological Process | urate transport | |
Biological Process | xanthine transport |
Keywords
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameUric acid transporter UacT
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionQ46821
- Secondary accessions
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Cell inner membrane ; Multi-pass membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-29 | Cytoplasmic | ||||
Sequence: MSAIDSQLPSSSGQDRPTDEVDRILSPGK | ||||||
Transmembrane | 30-50 | Helical | ||||
Sequence: LIILGLQHVLVMYAGAVAVPL | ||||||
Topological domain | 51-62 | Periplasmic | ||||
Sequence: MIGDRLGLSKEA | ||||||
Transmembrane | 63-83 | Helical | ||||
Sequence: IAMLISSDLFCCGIVTLLQCI | ||||||
Topological domain | 84-92 | Cytoplasmic | ||||
Sequence: GIGRFMGIR | ||||||
Transmembrane | 93-113 | Helical | ||||
Sequence: LPVIMSVTFAAVTPMIAIGMN | ||||||
Topological domain | 114-115 | Periplasmic | ||||
Sequence: PD | ||||||
Transmembrane | 116-136 | Helical | ||||
Sequence: IGLLGIFGATIAAGFITTLLA | ||||||
Topological domain | 137-142 | Cytoplasmic | ||||
Sequence: PLIGRL | ||||||
Transmembrane | 143-163 | Helical | ||||
Sequence: MPLFPPLVTGVVITSIGLSII | ||||||
Topological domain | 164-178 | Periplasmic | ||||
Sequence: QVGIDWAAGGKGNPQ | ||||||
Transmembrane | 179-199 | Helical | ||||
Sequence: YGNPVYLGISFAVLIFILLIT | ||||||
Topological domain | 200-204 | Cytoplasmic | ||||
Sequence: RYAKG | ||||||
Transmembrane | 205-225 | Helical | ||||
Sequence: FMSNVAVLLGIVFGFLLSWMM | ||||||
Topological domain | 226-261 | Periplasmic | ||||
Sequence: NEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMT | ||||||
Transmembrane | 262-282 | Helical | ||||
Sequence: AVLIIVFIESMGMFLALGEIV | ||||||
Topological domain | 283-337 | Cytoplasmic | ||||
Sequence: GRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVCISS | ||||||
Transmembrane | 338-358 | Helical | ||||
Sequence: GIILILFGMVPKMAVLVASIP | ||||||
Topological domain | 359 | Periplasmic | ||||
Sequence: Q | ||||||
Transmembrane | 360-380 | Helical | ||||
Sequence: FVLGGAGLVMFGMVLATGIRI | ||||||
Topological domain | 381-392 | Cytoplasmic | ||||
Sequence: LSRCNYTTNRYN | ||||||
Transmembrane | 393-413 | Helical | ||||
Sequence: LYIVAISLGVGMTPTLSHDFF | ||||||
Topological domain | 414-421 | Periplasmic | ||||
Sequence: SKLPAVLQ | ||||||
Transmembrane | 422-442 | Helical | ||||
Sequence: PLLHSGIMLATLSAVVLNVFF | ||||||
Topological domain | 443-482 | Cytoplasmic | ||||
Sequence: NGYQHHADLVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE |
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 37 | Lack of activity. | ||||
Sequence: H → K or L | ||||||
Mutagenesis | 37 | Strong decrease in activity. | ||||
Sequence: H → N | ||||||
Mutagenesis | 100 | Decrease in activity with uric acid. Highly active with xanthine. | ||||
Sequence: T → A | ||||||
Mutagenesis | 100 | Decrease in activity. | ||||
Sequence: T → S | ||||||
Mutagenesis | 256 | Almost no change in activity. | ||||
Sequence: S → Q | ||||||
Mutagenesis | 259 | Almost no change in activity. | ||||
Sequence: T → V | ||||||
Mutagenesis | 270 | Lack of activity. | ||||
Sequence: E → D, N, or Q | ||||||
Mutagenesis | 278 | Almost no change in activity. | ||||
Sequence: L → T | ||||||
Mutagenesis | 298 | Strong decrease in activity. | ||||
Sequence: D → E | ||||||
Mutagenesis | 298 | Lack of activity. | ||||
Sequence: D → N | ||||||
Mutagenesis | 317 | Decrease in activity. | ||||
Sequence: S → A | ||||||
Mutagenesis | 318 | Strong decrease in activity. | ||||
Sequence: Q → E or N | ||||||
Mutagenesis | 319 | Lack of activity. | ||||
Sequence: N → D or Q | ||||||
Mutagenesis | 320 | Lack of activity. | ||||
Sequence: V → N | ||||||
Mutagenesis | 327 | Almost no change in activity. | ||||
Sequence: R → G | ||||||
Mutagenesis | 426 | Almost no change in activity. | ||||
Sequence: S → N |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000165968 | 1-482 | Uric acid transporter UacT | |||
Sequence: MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAGAVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFAAVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGLSIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFGFLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFLALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVCISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYNLYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHADLVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE |
Proteomic databases
Structure
Sequence
- Sequence statusComplete
- Length482
- Mass (Da)51,758
- Last updated2000-05-30 v2
- ChecksumE08EF1D4CBD9D066
Sequence caution
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U28375 EMBL· GenBank· DDBJ | AAA83069.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation | |
U00096 EMBL· GenBank· DDBJ | AAC75926.2 EMBL· GenBank· DDBJ | Genomic DNA | ||
AP009048 EMBL· GenBank· DDBJ | BAE76953.1 EMBL· GenBank· DDBJ | Genomic DNA |