Q46720 · Q46720_ECOLX
- ProteinGDP-L-fucose synthase
- GenewbdJ
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids307 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the two-step NADP-dependent conversion of GDP-4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction.
Catalytic activity
- GDP-beta-L-fucose + NADP+ = GDP-4-dehydro-alpha-D-rhamnose + NADPH + H+
Pathway
Nucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis via de novo pathway; GDP-L-fucose from GDP-alpha-D-mannose: step 2/2.
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 7-13 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: GSTGMVG | ||||||
Binding site | 101-104 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: LGSS | ||||||
Site | 103 | Important for catalytic activity | ||||
Sequence: S | ||||||
Site | 105 | Important for catalytic activity | ||||
Sequence: C | ||||||
Active site | 132 | Proton donor/acceptor | ||||
Sequence: Y | ||||||
Binding site | 136 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 160-163 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: PCNL | ||||||
Binding site | 176 | NADP+ (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 184 | substrate | ||||
Sequence: K | ||||||
Binding site | 199 | substrate | ||||
Sequence: W | ||||||
Binding site | 206 | substrate | ||||
Sequence: R |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | GDP-L-fucose synthase activity | |
Molecular Function | isomerase activity | |
Molecular Function | NADP+ binding | |
Biological Process | 'de novo' GDP-L-fucose biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGDP-L-fucose synthase
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Enterobacterales > Enterobacteriaceae > Escherichia
Accessions
- Primary accessionQ46720
Proteomes
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 3-235 | NAD-dependent epimerase/dehydratase | ||||
Sequence: VLLTGSTGMVGKNILEHDSASKYNILTPTSSDLNLLDKNEIEKFMLINMPDCIIHAAGLVGGIHANISRPFDFLEKNLQMGLNLVSVAKKLGIKKVLNLGSSCMYPKNFEEAIPEKALLTGELEETNEGYAIAKIAVAKACEYISRENSNYFYKTIIPCNLYGKYDKFDDNSSHMIPAVIKKIHHAKINNVPEIEIWGDGNSRREFMYAEDLADLIFYVIPKIEFMPNMVNAG |
Sequence similarities
Belongs to the NAD(P)-dependent epimerase/dehydratase family. Fucose synthase subfamily.
Family and domain databases
Sequence
- Sequence statusComplete
- Length307
- Mass (Da)34,751
- Last updated1996-11-01 v1
- Checksum16056E2D8348B289
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AF078736 EMBL· GenBank· DDBJ | AAC44884.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887644 EMBL· GenBank· DDBJ | AFE83059.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887645 EMBL· GenBank· DDBJ | AFE83070.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887646 EMBL· GenBank· DDBJ | AFE83081.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887647 EMBL· GenBank· DDBJ | AFE83092.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887648 EMBL· GenBank· DDBJ | AFE83103.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887649 EMBL· GenBank· DDBJ | AFE83114.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887650 EMBL· GenBank· DDBJ | AFE83125.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887651 EMBL· GenBank· DDBJ | AFE83136.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887652 EMBL· GenBank· DDBJ | AFE83147.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887653 EMBL· GenBank· DDBJ | AFE83158.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887654 EMBL· GenBank· DDBJ | AFE83169.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887655 EMBL· GenBank· DDBJ | AFE83180.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887656 EMBL· GenBank· DDBJ | AFE83191.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887657 EMBL· GenBank· DDBJ | AFE83201.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887658 EMBL· GenBank· DDBJ | AFE83212.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887659 EMBL· GenBank· DDBJ | AFE83223.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887660 EMBL· GenBank· DDBJ | AFE83234.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887661 EMBL· GenBank· DDBJ | AFE83245.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887662 EMBL· GenBank· DDBJ | AFE83256.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887663 EMBL· GenBank· DDBJ | AFE83267.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887664 EMBL· GenBank· DDBJ | AFE83278.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887665 EMBL· GenBank· DDBJ | AFE83289.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887666 EMBL· GenBank· DDBJ | AFE83300.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887667 EMBL· GenBank· DDBJ | AFE83311.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887668 EMBL· GenBank· DDBJ | AFE83322.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887669 EMBL· GenBank· DDBJ | AFE83333.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887670 EMBL· GenBank· DDBJ | AFE83344.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887671 EMBL· GenBank· DDBJ | AFE83355.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887672 EMBL· GenBank· DDBJ | AFE83366.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887673 EMBL· GenBank· DDBJ | AFE83377.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887674 EMBL· GenBank· DDBJ | AFE83388.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887675 EMBL· GenBank· DDBJ | AFE83399.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887676 EMBL· GenBank· DDBJ | AFE83410.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887677 EMBL· GenBank· DDBJ | AFE83421.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887678 EMBL· GenBank· DDBJ | AFE83432.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887679 EMBL· GenBank· DDBJ | AFE83443.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887680 EMBL· GenBank· DDBJ | AFE83454.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887681 EMBL· GenBank· DDBJ | AFE83465.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887682 EMBL· GenBank· DDBJ | AFE83476.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887683 EMBL· GenBank· DDBJ | AFE83487.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887684 EMBL· GenBank· DDBJ | AFE83498.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887685 EMBL· GenBank· DDBJ | AFE83509.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887686 EMBL· GenBank· DDBJ | AFE83520.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887687 EMBL· GenBank· DDBJ | AFE83531.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887688 EMBL· GenBank· DDBJ | AFE83542.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887689 EMBL· GenBank· DDBJ | AFE83553.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
JN887690 EMBL· GenBank· DDBJ | AFE83564.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
UARW01000010 EMBL· GenBank· DDBJ | SQD04757.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
UGFE01000002 EMBL· GenBank· DDBJ | STM23320.1 EMBL· GenBank· DDBJ | Genomic DNA |