Q45070 · XYNC_BACSU
- ProteinGlucuronoxylanase XynC
- GenexynC
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids422 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the depolymerization of methylglucuronoxylan (MeGAXn) from different sources. It cleaves the beta-1,4-xylosidic bond penultimate to that linking carbon one of the xylose residue substituted with alpha-1,2-linked 4-O-methyl-D-glucuronate (MeGA).
Catalytic activity
Kinetics
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
59.5 μmol/min/mg | 6 | 37 | with methylglucuronoxylan from sweetgum as substrate |
The Km value for methylglucuronoxylan from sweetgum is 1.63 mg/ml. The activity is directly correlated to the degree of substitution of the glucuronosyl moiety on the xylan chain.
pH Dependence
Optimum pH is 6.
Temperature Dependence
Optimum temperature is 65 degrees Celsius. Half the activity is retained for 25 hours at 40 degrees Celsius and for 5 hours at 50 degrees Celsius.
Pathway
Glycan degradation; xylan degradation.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 171 | Proton donor | ||||
Sequence: E | ||||||
Active site | 260 | Nucleophile | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Molecular Function | glucosylceramidase activity | |
Molecular Function | glucuronoarabinoxylan endo-1,4-beta-xylanase activity | |
Biological Process | sphingolipid metabolic process | |
Biological Process | xylan catabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameGlucuronoxylanase XynC
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Bacillota > Bacilli > Bacillales > Bacillaceae > Bacillus
Accessions
- Primary accessionQ45070
- Secondary accessions
Proteomes
Subcellular Location
PTM/Processing
Features
Showing features for signal, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-32 | |||||
Sequence: MIPRIKKTICVLLVCFTMLSVMLGPGATEVLA | ||||||
Chain | PRO_0000278646 | 33-422 | Glucuronoxylanase XynC | |||
Sequence: ASDVTVNVSAEKQVIRGFGGMNHPAWAGDLTAAQRETAFGNGQNQLGFSILRIHVDENRNNWYKEVETAKSAVKHGAIVFASPWNPPSDMVETFNRNGDTSAKRLKYNKYAAYAQHLNDFVTFMKNNGVNLYAISVQNEPDYAHEWTWWTPQEILRFMRENAGSINARVIAPESFQYLKNLSDPILNDPQALANMDILGTHLYGTQVSQFPYPLFKQKGAGKDLWMTEVYYPNSDTNSADRWPEALDVSQHIHNAMVEGDFQAYVWWYIRRSYGPMKEDGTISKRGYNMAHFSKFVRPGYVRIDATKNPNANVYVSAYKGDNKVVIVAINKSNTGVNQNFVLQNGSASNVSRWITSSSSNLQPGTNLTVSGNHFWAHLPAQSVTTFVVNR |
Proteomic databases
Expression
Induction
Constitutively expressed.
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Sequence similarities
Belongs to the glycosyl hydrolase 30 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length422
- Mass (Da)47,337
- Last updated1996-11-01 v1
- ChecksumFEEB750E3B9DAAB8
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
Z73234 EMBL· GenBank· DDBJ | CAA97612.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL009126 EMBL· GenBank· DDBJ | CAB13698.1 EMBL· GenBank· DDBJ | Genomic DNA |