Q45070 · XYNC_BACSU

Function

function

Catalyzes the depolymerization of methylglucuronoxylan (MeGAXn) from different sources. It cleaves the beta-1,4-xylosidic bond penultimate to that linking carbon one of the xylose residue substituted with alpha-1,2-linked 4-O-methyl-D-glucuronate (MeGA).

Catalytic activity

  • Endohydrolysis of (1->4)-beta-D-xylosyl links in some glucuronoarabinoxylans.
    EC:3.2.1.136 (UniProtKB | ENZYME | Rhea)

Kinetics

Vmax pH TEMPERATURE[C] NOTES EVIDENCE
59.5 μmol/min/mg637with methylglucuronoxylan from sweetgum as substrate
The Km value for methylglucuronoxylan from sweetgum is 1.63 mg/ml. The activity is directly correlated to the degree of substitution of the glucuronosyl moiety on the xylan chain.

pH Dependence

Optimum pH is 6.

Temperature Dependence

Optimum temperature is 65 degrees Celsius. Half the activity is retained for 25 hours at 40 degrees Celsius and for 5 hours at 50 degrees Celsius.

Pathway

Glycan degradation; xylan degradation.

Features

Showing features for active site.

TypeIDPosition(s)Description
Active site171Proton donor
Active site260Nucleophile

GO annotations

AspectTerm
Cellular Componentextracellular region
Molecular Functionglucosylceramidase activity
Molecular Functionglucuronoarabinoxylan endo-1,4-beta-xylanase activity
Biological Processsphingolipid metabolic process
Biological Processxylan catabolic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Glucuronoxylanase XynC
  • EC number
  • Alternative names
    • Endoxylanase XynC
    • Glucuronoxylan xylanohydrolase

Gene names

    • Name
      xynC
    • Synonyms
      ynfF
    • Ordered locus names
      BSU18150

Organism names

Accessions

  • Primary accession
    Q45070
  • Secondary accessions
    • Q796G4

Proteomes

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for signal, chain.

TypeIDPosition(s)Description
Signal1-32
ChainPRO_000027864633-422Glucuronoxylanase XynC

Proteomic databases

Expression

Induction

Constitutively expressed.

Interaction

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the glycosyl hydrolase 30 family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    422
  • Mass (Da)
    47,337
  • Last updated
    1996-11-01 v1
  • Checksum
    FEEB750E3B9DAAB8
MIPRIKKTICVLLVCFTMLSVMLGPGATEVLAASDVTVNVSAEKQVIRGFGGMNHPAWAGDLTAAQRETAFGNGQNQLGFSILRIHVDENRNNWYKEVETAKSAVKHGAIVFASPWNPPSDMVETFNRNGDTSAKRLKYNKYAAYAQHLNDFVTFMKNNGVNLYAISVQNEPDYAHEWTWWTPQEILRFMRENAGSINARVIAPESFQYLKNLSDPILNDPQALANMDILGTHLYGTQVSQFPYPLFKQKGAGKDLWMTEVYYPNSDTNSADRWPEALDVSQHIHNAMVEGDFQAYVWWYIRRSYGPMKEDGTISKRGYNMAHFSKFVRPGYVRIDATKNPNANVYVSAYKGDNKVVIVAINKSNTGVNQNFVLQNGSASNVSRWITSSSSNLQPGTNLTVSGNHFWAHLPAQSVTTFVVNR

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
Z73234
EMBL· GenBank· DDBJ
CAA97612.1
EMBL· GenBank· DDBJ
Genomic DNA
AL009126
EMBL· GenBank· DDBJ
CAB13698.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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