Q44278 · GYRB_ACIS7

Function

function

A type II topoisomerase that negatively supercoils closed circular double-stranded (ds) DNA in an ATP-dependent manner to modulate DNA topology and maintain chromosomes in an underwound state. Negative supercoiling favors strand separation, and DNA replication, transcription, recombination and repair, all of which involve strand separation. Also able to catalyze the interconversion of other topological isomers of dsDNA rings, including catenanes and knotted rings. Type II topoisomerases break and join 2 DNA strands simultaneously in an ATP-dependent manner.

Miscellaneous

Few gyrases are as efficient as E.coli at forming negative supercoils. Not all organisms have 2 type II topoisomerases; in organisms with a single type II topoisomerase this enzyme also has to decatenate newly replicated chromosomes.

Catalytic activity

  • ATP-dependent breakage, passage and rejoining of double-stranded DNA.
    EC:5.6.2.2 (UniProtKB | ENZYME | Rhea)

Features

Showing features for site.

121620406080100120140160180200
TypeIDPosition(s)Description
Site171Interaction with DNA
Site174Interaction with DNA

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular FunctionATP binding
Molecular FunctionDNA binding
Molecular FunctionDNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
Biological ProcessDNA topological change

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    DNA gyrase subunit B
  • EC number

Gene names

    • Name
      gyrB

Organism names

Accessions

  • Primary accession
    Q44278
  • Secondary accessions
    • Q44269

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00001452861-216DNA gyrase subunit B

Interaction

Subunit

Heterotetramer, composed of two GyrA and two GyrB chains. In the heterotetramer, GyrA contains the active site tyrosine that forms a transient covalent intermediate with DNA, while GyrB binds cofactors and catalyzes ATP hydrolysis.

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain140-216Toprim

Sequence similarities

Belongs to the type II topoisomerase GyrB family.

Family and domain databases

Sequence

  • Sequence status
    Fragments
  • Length
    216
  • Mass (Da)
    23,742
  • Last updated
    1997-11-01 v1
  • Checksum
    48186007AD1D301B
SHKVSGGLHGVGVSVVNALSSKLQLTIHRAGQIHEQEYQHGDPQYPLKVVGETSTTGTTVRFWPSGDTFSQTIFNVDILARRLRELSFLNAGVKIVLRDERVNFEHIYAYEGGLSEKSALDIAGLPGKLADCQEKDPALSELYLVEGDSPGGSAKQGRNRKMQAILPLKGKILNVERARFDKMISSQEVGTLITALGCGIGREEYNPDKLRYHKII

Features

Showing features for non-terminal residue, non-adjacent residues.

TypeIDPosition(s)Description
Non-terminal residue1
Non-adjacent residues116-117
Non-terminal residue216

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
D73441
EMBL· GenBank· DDBJ
BAA11166.1
EMBL· GenBank· DDBJ
Genomic DNA
D73426
EMBL· GenBank· DDBJ
BAA11151.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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