Q42438 · CDPK8_ARATH
- ProteinCalcium-dependent protein kinase 8
- GeneCPK8
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids533 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
May play a role in signal transduction pathways that involve calcium as a second messenger.
Catalytic activity
- ATP + L-seryl-[protein] = ADP + H+ + O-phospho-L-seryl-[protein]
Activity regulation
Activated by calcium. Autophosphorylation may play an important role in the regulation of the kinase activity (By similarity).
Features
Showing features for binding site, active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 63-71 | ATP (UniProtKB | ChEBI) | ||||
Sequence: VGRGEFGIT | ||||||
Binding site | 86 | ATP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Active site | 181 | Proton acceptor | ||||
Sequence: D | ||||||
Binding site | 371 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 375 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 377 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 382 | Ca2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 408 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 410 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 412 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 414 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 419 | Ca2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 444 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 446 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: N | ||||||
Binding site | 448 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 450 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: Y | ||||||
Binding site | 455 | Ca2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 480 | Ca2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 482 | Ca2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 484 | Ca2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 486 | Ca2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: R | ||||||
Binding site | 491 | Ca2+ 4 (UniProtKB | ChEBI) | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | plasma membrane | |
Molecular Function | ATP binding | |
Molecular Function | calcium ion binding | |
Molecular Function | protein serine kinase activity | |
Molecular Function | protein serine/threonine kinase activity |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCalcium-dependent protein kinase 8
- EC number
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ42438
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
PTM/Processing
Features
Showing features for initiator methionine, lipidation, chain, modified residue.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Lipidation | 2 | N-myristoyl glycine | ||||
Sequence: G | ||||||
Chain | PRO_0000363333 | 2-533 | Calcium-dependent protein kinase 8 | |||
Sequence: GNCCASPGSETGSKKGKPKIKSNPFYSEAYTTNGSGTGFKLSVLKDPTGHDISLMYDLGREVGRGEFGITYLCTDIKTGEKYACKSISKKKLRTAVDIEDVRREVEIMKHMPRHPNIVSLKDAFEDDDAVHIVMELCEGGELFDRIVARGHYTERAAAAVMKTILEVVQICHKHGVMHRDLKPENFLFANKKETSALKAIDFGLSVFFKPGEGFNEIVGSPYYMAPEVLRRNYGPEVDIWSAGVILYILLCGVPPFWAETEQGVAQAIIRSVIDFKRDPWPRVSETAKDLVRKMLEPDPKKRLSAAQVLEHSWIQNAKKAPNVSLGETVKARLKQFSVMNKLKKRALRVIAEHLSVEEVAGIKEAFEMMDSKKTGKINLEELKFGLHKLGQQQIPDTDLQILMEAADVDGDGTLNYGEFVAVSVHLKKMANDEHLHKAFSFFDQNQSDYIEIEELREALNDEVDTNSEEVVAAIMQDVDTDKDGRISYEEFAAMMKAGTDWRKASRQYSRERFNSLSLKLMREGSLQLEGEN | ||||||
Modified residue | 221 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 488 | Phosphoserine | ||||
Sequence: S | ||||||
Modified residue | 526 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-21 | Disordered | ||||
Sequence: MGNCCASPGSETGSKKGKPKI | ||||||
Domain | 57-315 | Protein kinase | ||||
Sequence: YDLGREVGRGEFGITYLCTDIKTGEKYACKSISKKKLRTAVDIEDVRREVEIMKHMPRHPNIVSLKDAFEDDDAVHIVMELCEGGELFDRIVARGHYTERAAAAVMKTILEVVQICHKHGVMHRDLKPENFLFANKKETSALKAIDFGLSVFFKPGEGFNEIVGSPYYMAPEVLRRNYGPEVDIWSAGVILYILLCGVPPFWAETEQGVAQAIIRSVIDFKRDPWPRVSETAKDLVRKMLEPDPKKRLSAAQVLEHSWI | ||||||
Region | 321-351 | Autoinhibitory domain | ||||
Sequence: APNVSLGETVKARLKQFSVMNKLKKRALRVI | ||||||
Domain | 358-394 | EF-hand 1 | ||||
Sequence: EEVAGIKEAFEMMDSKKTGKINLEELKFGLHKLGQQQ | ||||||
Domain | 395-430 | EF-hand 2 | ||||
Sequence: IPDTDLQILMEAADVDGDGTLNYGEFVAVSVHLKKM | ||||||
Domain | 431-466 | EF-hand 3 | ||||
Sequence: ANDEHLHKAFSFFDQNQSDYIEIEELREALNDEVDT | ||||||
Domain | 467-502 | EF-hand 4 | ||||
Sequence: NSEEVVAAIMQDVDTDKDGRISYEEFAAMMKAGTDW |
Domain
There are 3 contiguous domains conserved in the CDPK subfamily: a kinase domain, an autoinhibitory (junction) domain and a calmodulin-like domain. The autoinhibitory domain (321-351) inactivates kinase activity under calcium-free conditions (By similarity).
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q42438-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length533
- Mass (Da)59,941
- Last updated1996-11-01 v1
- Checksum8E9D9DD090C9FD1A
Q42438-2
- Name2
- Differences from canonical
- 1-55: Missing
Features
Showing features for alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_036290 | 1-55 | in isoform 2 | |||
Sequence: Missing |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U20624 EMBL· GenBank· DDBJ | AAA67655.1 EMBL· GenBank· DDBJ | mRNA | ||
U20627 EMBL· GenBank· DDBJ | AAA67658.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF296837 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
CP002688 EMBL· GenBank· DDBJ | AED92708.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002688 EMBL· GenBank· DDBJ | AED92709.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK227066 EMBL· GenBank· DDBJ | BAE99123.1 EMBL· GenBank· DDBJ | mRNA |