Q42384 · PRL1_ARATH
- ProteinProtein pleiotropic regulatory locus 1
- GenePRL1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids486 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Pleiotropic regulator of glucose, stress and hormone responses. Also regulates cytochrome P450 CYP90A1/CPD. Coordinates the expression of hormone- and stress-related genes and genes related to cell wall modification and growth, leading to altered sugar-dependent growth and developmental responses. Component of the MAC complex that probably regulates defense responses through transcriptional control and thereby is essential for plant innate immunity. By suppressing the expression of several 1O2-responsive genes, PRL1 seems to play a major role in modulating responses of plants to environmental changes by interconnecting 1O2-mediated retrograde signaling with other signaling pathways. Acts as a negative regulator of SNF1-related protein kinases AKIN10 and AKIN11 via the inhibition of their interaction with SKP1/ASK1. Component of the CUL4-RBX1-DDB1-PRL1 E3 ubiquitin-protein ligase complex, PRL1 may function as the substrate recognition module within this complex, leading to the AKIN10 degradation.
Pathway
Protein modification; protein ubiquitination.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | Cul4-RING E3 ubiquitin ligase complex | |
Cellular Component | cytosol | |
Cellular Component | nucleus | |
Biological Process | cotyledon development | |
Biological Process | defense response to bacterium | |
Biological Process | defense response to fungus | |
Biological Process | fruit development | |
Biological Process | hormone-mediated signaling pathway | |
Biological Process | innate immune response | |
Biological Process | leaf development | |
Biological Process | mRNA splicing, via spliceosome | |
Biological Process | negative regulation of DNA-templated transcription | |
Biological Process | protein ubiquitination | |
Biological Process | proteolysis | |
Biological Process | response to glucose | |
Biological Process | root development | |
Biological Process | sugar mediated signaling pathway |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProtein pleiotropic regulatory locus 1
- Short namesProtein PRL1
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ42384
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
Phenotypes & Variants
Disruption phenotype
Hypersensitivity to glucose and sucrose. Enhanced sensitivity of plants to stress and to growth hormones including cytokinin, ethylene, abscisic acid, and auxin. Accumulation of sugars and starch in leaves, and root elongation. Cell elongation defects. Enhanced susceptibility to virulent and avirulent pathogens.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 31 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000051157 | 1-486 | Protein pleiotropic regulatory locus 1 | |||
Sequence: MPAPTTEIEPIEAQSLKKLSLKSLKRSLELFSPVHGQFPPPDPEAKQIRLSHKMKVAFGGVEPVVSQPPRQPDRINEQPGPSNALSLAAPEGSKSTQKGATESAIVVGPTLLRPILPKGLNYTGSSGKSTTIIPANVSSYQRNLSTAALMERIPSRWPRPEWHAPWKNYRVIQGHLGWVRSVAFDPSNEWFCTGSADRTIKIWDVATGVLKLTLTGHIEQVRGLAVSNRHTYMFSAGDDKQVKCWDLEQNKVIRSYHGHLSGVYCLALHPTLDVLLTGGRDSVCRVWDIRTKMQIFALSGHDNTVCSVFTRPTDPQVVTGSHDTTIKFWDLRYGKTMSTLTHHKKSVRAMTLHPKENAFASASADNTKKFSLPKGEFCHNMLSQQKTIINAMAVNEDGVMVTGGDNGSIWFWDWKSGHSFQQSETIVQPGSLESEAGIYAACYDNTGSRLVTCEADKTIKMWKEDENATPETHPINFKPPKEIRRF |
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Subunit
Component of the multiprotein assembly MOS4-associated complex (MAC) at least composed of MOS4, CDC5, PRL1 and PRP19 (PubMed:17575050, PubMed:19629177).
Interacts with CDC5 (PubMed:17575050).
Component of the CUL4-RBX1-DDB1-PRL1 E3 ubiquitin-protein ligase complex. Interacts with DDB1A through its DWD motif (PubMed:18223036).
Interacts with AKIN10, AKIN11 and PIPC (PubMed:10220464).
Interacts with KAP2 (PubMed:9765207).
Interacts with CDC5 (PubMed:17575050).
Component of the CUL4-RBX1-DDB1-PRL1 E3 ubiquitin-protein ligase complex. Interacts with DDB1A through its DWD motif (PubMed:18223036).
Interacts with AKIN10, AKIN11 and PIPC (PubMed:10220464).
Interacts with KAP2 (PubMed:9765207).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q42384 | CDC5 P92948 | 2 | EBI-1382964, EBI-1382948 | |
BINARY | Q42384 | IMPA3 O04294 | 3 | EBI-1382964, EBI-1644689 | |
BINARY | Q42384 | KIN10 Q38997-2 | 3 | EBI-1382964, EBI-20798606 | |
BINARY | Q42384 | KIN11 P92958 | 2 | EBI-1382964, EBI-307202 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, compositional bias, repeat, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 62-101 | Disordered | ||||
Sequence: EPVVSQPPRQPDRINEQPGPSNALSLAAPEGSKSTQKGAT | ||||||
Compositional bias | 71-101 | Polar residues | ||||
Sequence: QPDRINEQPGPSNALSLAAPEGSKSTQKGAT | ||||||
Repeat | 174-204 | WD 1 | ||||
Sequence: GHLGWVRSVAFDPSNEWFCTGSADRTIKIWD | ||||||
Repeat | 216-246 | WD 2 | ||||
Sequence: GHIEQVRGLAVSNRHTYMFSAGDDKQVKCWD | ||||||
Repeat | 258-288 | WD 3 | ||||
Sequence: GHLSGVYCLALHPTLDVLLTGGRDSVCRVWD | ||||||
Motif | 275-290 | DWD box 1 | ||||
Sequence: LLTGGRDSVCRVWDIR | ||||||
Repeat | 300-330 | WD 4 | ||||
Sequence: GHDNTVCSVFTRPTDPQVVTGSHDTTIKFWD | ||||||
Motif | 317-332 | DWD box 2 | ||||
Sequence: VVTGSHDTTIKFWDLR | ||||||
Repeat | 342-371 | WD 5 | ||||
Sequence: HHKKSVRAMTLHPKENAFASASADNTKKFS | ||||||
Repeat | 384-413 | WD 6 | ||||
Sequence: QQKTIINAMAVNEDGVMVTGGDNGSIWFWD | ||||||
Repeat | 433-463 | WD 7 | ||||
Sequence: ESEAGIYAACYDNTGSRLVTCEADKTIKMWK | ||||||
Region | 465-486 | Disordered | ||||
Sequence: DENATPETHPINFKPPKEIRRF |
Domain
The DWD box is required for interaction with DDB1A.
Sequence similarities
Belongs to the WD repeat PRL1/PRL2 family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length486
- Mass (Da)54,009
- Last updated1996-11-01 v1
- Checksum604D3E6FCDA8A998
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 71-101 | Polar residues | ||||
Sequence: QPDRINEQPGPSNALSLAAPEGSKSTQKGAT |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
X82824 EMBL· GenBank· DDBJ | CAA58031.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
X82825 EMBL· GenBank· DDBJ | CAA58032.1 EMBL· GenBank· DDBJ | mRNA | ||
Z97339 EMBL· GenBank· DDBJ | CAB10369.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL161542 EMBL· GenBank· DDBJ | CAB78632.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002687 EMBL· GenBank· DDBJ | AEE83664.1 EMBL· GenBank· DDBJ | Genomic DNA |