Q41364 · DIT1_SPIOL
- ProteinDicarboxylate transporter 1, chloroplastic
- GeneDIT1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids569 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
2-oxoglutarate/malate translocator that transports carbon skeletons into chloroplasts for net glutamate synthesis. This translocator exchanges malate for internal succinate, fumarate and 2-oxoglutarate but not for aspartate and glutamate. Involved with DIT2 in primary ammonia assimilation and in the re-assimilation of ammonia generated by the photorespiratory pathway. Imports 2-oxoglutarate into plastids as precursor for ammonia assimilation. 2-oxoglutarate is converted to glutamate, the end product of ammonia assimilation, which is exported to the cytosol by DIT2.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
0.35 mM | malate | |||||
0.17 mM | 2-oxoglutarate | |||||
0.04 mM | oxaloacetate |
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | chloroplast inner membrane | |
Molecular Function | alpha-ketoglutarate transmembrane transporter activity | |
Molecular Function | malate transmembrane transporter activity | |
Biological Process | alpha-ketoglutarate transport | |
Biological Process | malate transport |
Keywords
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameDicarboxylate transporter 1, chloroplastic
- Short namesSODIT1
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > Caryophyllales > Chenopodiaceae > Chenopodioideae > Anserineae > Spinacia
Accessions
- Primary accessionQ41364
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Plastid, chloroplast inner membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 106-126 | Helical | ||||
Sequence: PLLASILTGVIIWFIPTPEGV | ||||||
Transmembrane | 134-154 | Helical | ||||
Sequence: LAIFLSTIVGIITQPLPLGAV | ||||||
Transmembrane | 172-192 | Helical | ||||
Sequence: AAFSAFGDPIPWLIALAFFFA | ||||||
Transmembrane | 241-261 | Helical | ||||
Sequence: AGGIFLPLVKSLCIACGSNVG | ||||||
Transmembrane | 268-288 | Helical | ||||
Sequence: LGAWLMLTCFQTSVISSSMFL | ||||||
Transmembrane | 317-337 | Helical | ||||
Sequence: AAFVPGLVSLIVVPLLLYVVY | ||||||
Transmembrane | 367-387 | Helical | ||||
Sequence: IMAVTLLLTVGLWVFGGKLGV | ||||||
Transmembrane | 388-408 | Helical | ||||
Sequence: DAVTAAILGLSVLLITGVVTW | ||||||
Transmembrane | 423-443 | Helical | ||||
Sequence: WFAALIAMAGYLNKYGLITWF | ||||||
Transmembrane | 450-470 | Helical | ||||
Sequence: VVGGLGLSWQMSFGVLVLLYF | ||||||
Transmembrane | 490-510 | Helical | ||||
Sequence: FLSVASALGTPPFLAAIVLSF | ||||||
Transmembrane | 543-563 | Helical | ||||
Sequence: YGFLISIVNLIIWLGVGGLWW |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for transit peptide, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transit peptide | 1-93 | Chloroplast | ||||
Sequence: MASMALSLTSSPTYSLSFRSLPSLKPLSKSQPSISLPSLRSNASKSPSLSHKHFLSPPSLLLPHKLKPISASSPTNPPPPPAPVPSPAPVSAP | ||||||
Chain | PRO_0000032663 | 94-569 | Dicarboxylate transporter 1, chloroplastic | |||
Sequence: AQVQPWQGASIKPLLASILTGVIIWFIPTPEGVSRNAWQLLAIFLSTIVGIITQPLPLGAVALMGLGASVLTKTLTFSAAFSAFGDPIPWLIALAFFFARGFIKTGLGNRIAYQFVKLFGSSSLGLGYSLVFSEALLAPAIPSVSARAGGIFLPLVKSLCIACGSNVGDGTERKLGAWLMLTCFQTSVISSSMFLTAMAANPLSATLTFNTIGKAIGWMDWAKAAFVPGLVSLIVVPLLLYVVYPPEIKSSPDAPRLAKEKLDKMGPMTKNESIMAVTLLLTVGLWVFGGKLGVDAVTAAILGLSVLLITGVVTWKECLAESVAWDTLTWFAALIAMAGYLNKYGLITWFSENVVKVVGGLGLSWQMSFGVLVLLYFYSHYFFASGAAHIGAMFTAFLSVASALGTPPFLAAIVLSFLSNLMGGLTHYGIGSAPVFYGANYVPLPQWWGYGFLISIVNLIIWLGVGGLWWKAIGLW |
Post-translational modification
The N-terminus is blocked.
Expression
Tissue specificity
Expressed in leaves.
Induction
Circadian regulation with a peak in the middle of the light period.
Interaction
Subunit
Monomer.
Structure
Family & Domains
Features
Showing features for compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 23-53 | Polar residues | ||||
Sequence: SLKPLSKSQPSISLPSLRSNASKSPSLSHKH | ||||||
Region | 23-93 | Disordered | ||||
Sequence: SLKPLSKSQPSISLPSLRSNASKSPSLSHKHFLSPPSLLLPHKLKPISASSPTNPPPPPAPVPSPAPVSAP | ||||||
Compositional bias | 72-92 | Pro residues | ||||
Sequence: SSPTNPPPPPAPVPSPAPVSA |
Sequence similarities
Belongs to the SLC13A/DASS transporter (TC 2.A.47) family. DIT1 subfamily.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length569
- Mass (Da)60,288
- Last updated1996-11-01 v1
- Checksum5BF756490B1F7AA4
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 23-53 | Polar residues | ||||
Sequence: SLKPLSKSQPSISLPSLRSNASKSPSLSHKH | ||||||
Compositional bias | 72-92 | Pro residues | ||||
Sequence: SSPTNPPPPPAPVPSPAPVSA |
Keywords
- Technical term