Q41131 · FAH12_RICCO

Function

function

Oleate hydroxylase involved in the biosynthesis of ricinoleate (PubMed:27056057, PubMed:7624314).
It uses cytochrome b5 as an electron donor (PubMed:1417766).
May act on both oleic acid (18:1(9cis)) and eicosenoic acid (20:1(11cis)) (PubMed:8784737).

Catalytic activity

Activity regulation

Inhibited by oleoyloxyethyl phosphocholine.

Biotechnology

Promotes the accumulation of hydroxy fatty acids when expressed in A.thaliana.

pH Dependence

Optimum pH is 6.3.

Temperature Dependence

Optimum temperature is 22.5 degrees Celsius.

Pathway

Lipid metabolism; monounsaturated fatty acid biosynthesis.

GO annotations

AspectTerm
Cellular Componentendoplasmic reticulum membrane
Molecular Functionmonooxygenase activity
Molecular Functionoxidoreductase activity
Molecular Functionoxidoreductase activity, acting on paired donors, with oxidation of a pair of donors resulting in the reduction of molecular oxygen to two molecules of water
Molecular Functionphosphatidylcholine 12-monooxygenase activity
Biological Processfatty acid biosynthetic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Oleate hydroxylase FAH12
  • EC number
  • Short names
    RcFAH12
  • Alternative names
    • Oleate Delta(12)-hydroxylase
    • Phosphatidylcholine 12-monooxygenase
      (EC:1.14.18.4
      ) . EC:1.14.18.4 (UniProtKB | ENZYME | Rhea)

Gene names

    • Name
      FAH12
    • ORF names
      RCOM_0146820

Organism names

  • Taxonomic identifier
  • Strains
    • cv. Baker 296
    • cv. Hale
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > fabids > Malpighiales > Euphorbiaceae > Acalyphoideae > Acalypheae > Ricinus

Accessions

  • Primary accession
    Q41131
  • Secondary accessions
    • B9SQ58
    • D2CPE1

Proteomes

Subcellular Location

Microsome membrane
; Multi-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane61-81Helical
Transmembrane88-108Helical
Transmembrane121-141Helical
Transmembrane183-203Helical
Transmembrane229-249Helical
Transmembrane253-273Helical

Keywords

Phenotypes & Variants

Features

Showing features for natural variant, mutagenesis.

TypeIDPosition(s)Description
Natural variant49in OLE-1; enhanced accumulation of oleic acid but low ricinoleic acid levels in seeds, high-oleic castor mutant 1; when associated with A-242 and Q-319
Mutagenesis49Strongly reduced ricinoleic acid accumulation; when associated with A-242.
Natural variant242in OLE-1; enhanced accumulation of oleic acid but low ricinoleic acid levels in seeds, high-oleic castor mutant 1; when associated with S-49 and Q-319
Mutagenesis242Lower ricinoleic acid accumulation. Strongly reduced ricinoleic acid accumulation; when associated with S-49 or Q-319.
Natural variant319in OLE-1; enhanced accumulation of oleic acid but low ricinoleic acid levels in seeds, high-oleic castor mutant 1; when associated with S-49 and A-242
Mutagenesis319Strongly reduced ricinoleic acid accumulation; when associated with A-242.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 3 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004346331-387Oleate hydroxylase FAH12

Expression

Tissue specificity

Expressed in seeds. Barely detected in leaves.

Developmental stage

Accumulates progressively during seeds development.

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for region, motif.

TypeIDPosition(s)Description
Region1-34Disordered
Motif109-113Histidine box-1
Motif145-149Histidine box-2
Motif319-323Histidine box-3

Domain

The histidine box domains may contain the active site and/or be involved in metal ion binding.

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    387
  • Mass (Da)
    44,435
  • Last updated
    2018-02-28 v2
  • Checksum
    85C582CC65FE37D9
MGGGGRMSTVITSNNSEKKGGSSHLKRAPHTKPPFTLGDLKRAIPPHCFERSFVRSFSYVAYDVCLSFLFYSIATNFFPYISSPLSYVAWLVYWLFQGCILTGLWVIGHECGHHAFSEYQLADDIVGLIVHSALLVPYFSWKYSHRRHHSNIGSLERDEVFVPKSKSKISWYSKYLNNPPGRVLTLAATLLLGWPLYLAFNVSGRPYDRFACHYDPYGPIFSERERLQIYIADLGIFATTFVLYQATMAKGLAWVMRIYGVPLLIVNCFLVMITYLQHTHPAIPRYGSSEWDWLRGAMVTVDRDYGVLNKVFHNIADTHVAHHLFATVPHYHAMEATKAIKPIMGEYYRYDGTPFYKALWREAKECLFVEPDEGAPTQGVFWYRNKY

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict176in Ref. 1; AAC49010

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U22378
EMBL· GenBank· DDBJ
AAC49010.1
EMBL· GenBank· DDBJ
mRNA
EU523112
EMBL· GenBank· DDBJ
ACD39348.1
EMBL· GenBank· DDBJ
mRNA
EQ974077
EMBL· GenBank· DDBJ
EEF34257.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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