Q3ZAS0 · SL9A2_MOUSE
- ProteinSodium/hydrogen exchanger 2
- GeneSlc9a2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids814 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Plasma membrane Na+/H+ antiporter (PubMed:14962844, PubMed:16690903).
Mediates the electroneutral exchange of intracellular H+ ions for extracellular Na+ (PubMed:14962844).
Major apical Na+/H+ exchanger in the base of the colonic crypt (PubMed:14962844, PubMed:16690903).
Controls in the colonic crypt intracellular pH (pHi) to direct colonic epithelial cell differentiation into the absorptive enterocyte lineage at the expense of the secretory lineage (PubMed:14962844, PubMed:34985202).
Mediates the electroneutral exchange of intracellular H+ ions for extracellular Na+ (PubMed:14962844).
Major apical Na+/H+ exchanger in the base of the colonic crypt (PubMed:14962844, PubMed:16690903).
Controls in the colonic crypt intracellular pH (pHi) to direct colonic epithelial cell differentiation into the absorptive enterocyte lineage at the expense of the secretory lineage (PubMed:14962844, PubMed:34985202).
Catalytic activity
- H+(out) + Na+(in) = H+(in) + Na+(out)
Activity regulation
In colonic crypts activated by low pHi, hyperosmolarity, and an increase in intracellular cAMP levels.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | apical plasma membrane | |
Cellular Component | brush border | |
Cellular Component | plasma membrane | |
Molecular Function | potassium:proton antiporter activity | |
Molecular Function | sodium:proton antiporter activity | |
Biological Process | epithelial cell differentiation | |
Biological Process | potassium ion transmembrane transport | |
Biological Process | protein localization | |
Biological Process | regulation of intracellular pH | |
Biological Process | regulation of pH | |
Biological Process | sodium ion import across plasma membrane | |
Biological Process | sodium ion transmembrane transport | |
Biological Process | sodium ion transport |
Keywords
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameSodium/hydrogen exchanger 2
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ3ZAS0
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Apical cell membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 108-128 | Helical | ||||
Sequence: IVPESCLLIMVGLLLGGIIFG | ||||||
Transmembrane | 139-159 | Helical | ||||
Sequence: TDVFFLYLLPPIVLDAGYFMP | ||||||
Transmembrane | 170-190 | Helical | ||||
Sequence: IFWYAVVGTLWNSIGIGVSLF | ||||||
Transmembrane | 210-230 | Helical | ||||
Sequence: LFGSLISAVDPVAVLAVFENI | ||||||
Transmembrane | 238-258 | Helical | ||||
Sequence: ILVFGESLLNDAVTVVLYNLF | ||||||
Transmembrane | 279-299 | Helical | ||||
Sequence: FFVVGIGGVLIGILLGFIAAF | ||||||
Transmembrane | 309-329 | Helical | ||||
Sequence: VIEPLFVFLYSYLSYITAEMF | ||||||
Transmembrane | 362-382 | Helical | ||||
Sequence: YFMKMLSSVSETLIFIFMGVS | ||||||
Transmembrane | 393-413 | Helical | ||||
Sequence: AFVCFTLAFCLIWRALGVFVL | ||||||
Transmembrane | 431-451 | Helical | ||||
Sequence: FIIAYGGLRGAICFALVFLLP | ||||||
Transmembrane | 460-480 | Helical | ||||
Sequence: LFITAAIVVIFFTVFILGITI |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
SLC9A2 null mice do not develop diarrhea, have no changes in quantitated intestinal Na+ absorption, however mice develop a sharp reduction in the number of gastric parietal and zymogenic cells associated with a loss of net acid secretion in adult but not in juvenile mice (PubMed:11099134, PubMed:9502765).
SLC9A2 null mice also exhibited reduced recovery from laser-induced ulceration of the stomach (PubMed:21900251).
Double-knockout of SLC9A2 and SLC9A3 are not significantly different than the SLC9A3-deficient alone in diarrhea, intestinal Na+ absorption, or reduced life expectancy (PubMed:11705743).
Steady-state pH intracellular is significantly altered along the colonic crypt axis in deficicent mice (PubMed:34985202).
SLC9A2 null mice also exhibited reduced recovery from laser-induced ulceration of the stomach (PubMed:21900251).
Double-knockout of SLC9A2 and SLC9A3 are not significantly different than the SLC9A3-deficient alone in diarrhea, intestinal Na+ absorption, or reduced life expectancy (PubMed:11705743).
Steady-state pH intracellular is significantly altered along the colonic crypt axis in deficicent mice (PubMed:34985202).
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 20 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_5015097548 | 1-814 | Sodium/hydrogen exchanger 2 | |||
Sequence: MGPRGTAHSVRAPLSWLLLLLLSLQVAVPAGALAETLLDAPRARGASSNPPSPASVVAPGTTPFEESRLPVFTLDYPHVQIPFEITLWILLASLAKIGFHLYHKLPTIVPESCLLIMVGLLLGGIIFGVDEKSPPAMKTDVFFLYLLPPIVLDAGYFMPTRPFFENFGTIFWYAVVGTLWNSIGIGVSLFGICQIEAFGLSDITLLQNLLFGSLISAVDPVAVLAVFENIHVNEQLYILVFGESLLNDAVTVVLYNLFKSFCQMKTIQTVDVFAGIANFFVVGIGGVLIGILLGFIAAFTTRFTHNIRVIEPLFVFLYSYLSYITAEMFHLSGIMAITACAMTMNKYVEENVSQKSYTTIKYFMKMLSSVSETLIFIFMGVSTVGKNHEWNWAFVCFTLAFCLIWRALGVFVLTQVINWFRTIPLTFKDQFIIAYGGLRGAICFALVFLLPATVFPRKKLFITAAIVVIFFTVFILGITIRPLVEFLDVKRSNKKQQAVSEEIHCRFFDHVKTGIEDVCGHWGHNFWRDKFKKFDDKYLRKLLIRENQPKSSIVSLYKKLEIKHAIEMAETGMISTVPSFASLNDCREEKIRKLTPGEMDEIREILSRNLYQIRQRTLSYNRHNLTADTSERQAKEILIRRRHSLRESLRKDNSSNRERRASTSTSRYLSLPKNTKLPEKLQKKKKVSNADGNSSDSDPDVGTTVLNLQPRTRRFLPDQFSKKASQAYKMEWKNEVDVGSVRVPPSVSPAPRSKDGGTQTPGLLRQPLLSKDQRFGRGREDSLTEDVPPKPPPRLVRRASEPGNRKGRLGNEKP |
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in the colonic epithelium predominantly in the cryptal region. Maximally expressed in the luminal membrane near the crypt base and in the middle of the crypt (at protein level) (PubMed:14962844).
Expressed in gastric epithelium (at protein level) (PubMed:21900251).
Expressed in gastric epithelium (at protein level) (PubMed:21900251).
Gene expression databases
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 647-710 | Disordered | ||||
Sequence: ESLRKDNSSNRERRASTSTSRYLSLPKNTKLPEKLQKKKKVSNADGNSSDSDPDVGTTVLNLQP | ||||||
Compositional bias | 677-691 | Basic and acidic residues | ||||
Sequence: LPEKLQKKKKVSNAD | ||||||
Compositional bias | 692-710 | Polar residues | ||||
Sequence: GNSSDSDPDVGTTVLNLQP | ||||||
Region | 740-814 | Disordered | ||||
Sequence: SVRVPPSVSPAPRSKDGGTQTPGLLRQPLLSKDQRFGRGREDSLTEDVPPKPPPRLVRRASEPGNRKGRLGNEKP | ||||||
Compositional bias | 752-766 | Polar residues | ||||
Sequence: RSKDGGTQTPGLLRQ | ||||||
Compositional bias | 772-787 | Basic and acidic residues | ||||
Sequence: DQRFGRGREDSLTEDV | ||||||
Compositional bias | 799-814 | Basic and acidic residues | ||||
Sequence: ASEPGNRKGRLGNEKP |
Sequence similarities
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length814
- Mass (Da)91,646
- Last updated2005-09-27 v1
- Checksum8543A204ED2528C2
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
Q3TM63 | Q3TM63_MOUSE | Slc9a2 | 336 |
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 677-691 | Basic and acidic residues | ||||
Sequence: LPEKLQKKKKVSNAD | ||||||
Compositional bias | 692-710 | Polar residues | ||||
Sequence: GNSSDSDPDVGTTVLNLQP | ||||||
Compositional bias | 752-766 | Polar residues | ||||
Sequence: RSKDGGTQTPGLLRQ | ||||||
Compositional bias | 772-787 | Basic and acidic residues | ||||
Sequence: DQRFGRGREDSLTEDV | ||||||
Compositional bias | 799-814 | Basic and acidic residues | ||||
Sequence: ASEPGNRKGRLGNEKP |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AC154392 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC103685 EMBL· GenBank· DDBJ | AAI03686.1 EMBL· GenBank· DDBJ | mRNA | ||
BC104737 EMBL· GenBank· DDBJ | AAI04738.1 EMBL· GenBank· DDBJ | mRNA |