Q3V7S1 · MOAA_RHOPA
- ProteinGTP 3',8-cyclase
- GenemoaA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids344 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate.
Catalytic activity
- GTP + AH2 + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + L-methionine + A + H+
Cofactor
Note: Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.
Pathway
Cofactor biosynthesis; molybdopterin biosynthesis.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 28 | GTP (UniProtKB | ChEBI) | |||
Binding site | 35 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 39 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 41 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 42 | [4Fe-4S] cluster 1 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet | |||
Binding site | 77 | GTP (UniProtKB | ChEBI) | |||
Binding site | 81 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 111 | GTP (UniProtKB | ChEBI) | |||
Binding site | 135 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 171 | GTP (UniProtKB | ChEBI) | |||
Binding site | 205 | S-adenosyl-L-methionine (UniProtKB | ChEBI) | |||
Binding site | 268 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-substrate | |||
Binding site | 271 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-substrate | |||
Binding site | 273-275 | GTP (UniProtKB | ChEBI) | |||
Binding site | 285 | [4Fe-4S] cluster 2 (UniProtKB | ChEBI); 4Fe-4S-substrate | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | 4 iron, 4 sulfur cluster binding | |
Molecular Function | cyclic pyranopterin monophosphate synthase activity | |
Molecular Function | GTP 3',8'-cyclase activity | |
Molecular Function | GTP binding | |
Molecular Function | metal ion binding | |
Molecular Function | S-adenosyl-L-methionine binding | |
Biological Process | Mo-molybdopterin cofactor biosynthetic process |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameGTP 3',8-cyclase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Nitrobacteraceae > Rhodopseudomonas
Accessions
- Primary accessionQ3V7S1
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_1000054218 | 1-344 | GTP 3',8-cyclase | ||
Interaction
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 19-239 | Radical SAM core | |||
Sequence similarities
Belongs to the radical SAM superfamily. MoaA family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length344
- Mass (Da)37,859
- Last updated2005-10-11 v1
- MD5 Checksum823A22748B82FA8258292F3F6620834A
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BX572599 EMBL· GenBank· DDBJ | CAE27419.1 EMBL· GenBank· DDBJ | Genomic DNA |