Q3V5I7 · ATG5_CAEEL
- ProteinAutophagy-related protein 5
- Geneatg-5
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids275 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in autophagic vesicle formation (By similarity).
Conjugation with lgg-3/ATG12, through a ubiquitin-like conjugating system involving atg-7 as an E1-like activating enzyme and atg-10 as an E2-like conjugating enzyme, is essential for its function (By similarity).
Most likely a component of an atg-5-lgg-3-atg-16 complex that promotes autophagosome formation by associating with lgg-2, but not lgg-1, at the preautophagosomal membrane (PubMed:24185444).
Probably, as part of an atg-5-lgg-3-atg-16 complex, required for lgg-1 lipidation; the complex acts as an E3-like enzyme promoting atg-3-mediated lgg-1 lipidation (Probable). Furthermore, association with atg-16.2 is required for the nucleation of lgg-1 positive autophagic vesicles (PubMed:24185444).
Conjugation with lgg-3/ATG12, through a ubiquitin-like conjugating system involving atg-7 as an E1-like activating enzyme and atg-10 as an E2-like conjugating enzyme, is essential for its function (By similarity).
Most likely a component of an atg-5-lgg-3-atg-16 complex that promotes autophagosome formation by associating with lgg-2, but not lgg-1, at the preautophagosomal membrane (PubMed:24185444).
Probably, as part of an atg-5-lgg-3-atg-16 complex, required for lgg-1 lipidation; the complex acts as an E3-like enzyme promoting atg-3-mediated lgg-1 lipidation (Probable). Furthermore, association with atg-16.2 is required for the nucleation of lgg-1 positive autophagic vesicles (PubMed:24185444).
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | Atg12-Atg5-Atg16 complex | |
Cellular Component | autophagosome | |
Cellular Component | cytoplasm | |
Cellular Component | cytosol | |
Cellular Component | mitochondria-associated endoplasmic reticulum membrane contact site | |
Cellular Component | phagophore | |
Cellular Component | phagophore assembly site membrane | |
Cellular Component | transferase complex | |
Biological Process | autophagosome assembly | |
Biological Process | autophagy | |
Biological Process | autophagy of mitochondrion | |
Biological Process | cellular response to nitrogen starvation | |
Biological Process | macroautophagy | |
Biological Process | piecemeal microautophagy of the nucleus | |
Biological Process | regulation of autophagy |
Keywords
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameAutophagy-related protein 5
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Ecdysozoa > Nematoda > Chromadorea > Rhabditida > Rhabditina > Rhabditomorpha > Rhabditoidea > Rhabditidae > Peloderinae > Caenorhabditis
Accessions
- Primary accessionQ3V5I7
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Preautophagosomal structure membrane ; Peripheral membrane protein
Keywords
- Cellular component
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 130 | In bp546; results in defective degradation of protein aggregates by autophagy. | ||||
Sequence: E → K |
PTM/Processing
Features
Showing features for chain, cross-link.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000447607 | 1-275 | Autophagy-related protein 5 | |||
Sequence: MDYEVCRKVWESHVPCQFTLQSSGGTHGEPLPFYTMLPRFSYLALAIQKVLSSFNRRDDGEKVHSDKMWLEHNGIPLKMYIPIGVIYDQANLSENDSILEIIVRTSQPPPQFQMVDRDMMEAMFMQNIKEADYLKTKAEITKNMMKDESAQLWRSVCNIPDNFDEFWTIVQKLMETSEGNEFAHIPLRVYVKNQAFKQALITAKHPDGSLRTIGEAVSDVLSSSSSSSTDSQSEHPPRLISHGIDIPHHTPLIFAAKNLSYPDNFIHVVLLLVVP | ||||||
Cross-link | 129 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in lgg-3/ATG12) | ||||
Sequence: K |
Post-translational modification
Conjugated to lgg-3/ATG12; which is essential for autophagy.
Keywords
- PTM
Proteomic databases
Expression
Gene expression databases
Interaction
Subunit
Most likely a component of a complex at least containing atg-5, lgg-3/ATG12, atg-16.1 and/or atg-16.2 (Probable). Interacts with lgg-3/ATG12 (By similarity).
Interacts with atg-16.1 (via N-terminus) and atg-16.2 (via N-terminus) (PubMed:24185444).
Interacts with atg-16.1 (via N-terminus) and atg-16.2 (via N-terminus) (PubMed:24185444).
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 221-236 | Polar residues | ||||
Sequence: LSSSSSSSTDSQSEHP | ||||||
Region | 221-241 | Disordered | ||||
Sequence: LSSSSSSSTDSQSEHPPRLIS |
Sequence similarities
Belongs to the ATG5 family.
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 3 isoforms produced by Alternative splicing.
Q3V5I7-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Namea
- Length275
- Mass (Da)31,339
- Last updated2011-03-08 v2
- Checksum5D6691CB9FF94D42
Q3V5I7-2
- Nameb
Q3V5I7-3
- Namec
- Differences from canonical
- 159-161: IPD → N
Features
Showing features for alternative sequence, compositional bias.
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BX284601 EMBL· GenBank· DDBJ | CCD67969.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284601 EMBL· GenBank· DDBJ | CCD67987.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
BX284601 EMBL· GenBank· DDBJ | CDM63580.1 EMBL· GenBank· DDBJ | Genomic DNA |