Q3U0D9 · HACE1_MOUSE
- ProteinE3 ubiquitin-protein ligase HACE1
- GeneHace1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids909 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens (By similarity).
May also act as a transcription regulator via its interaction with RARB
May also act as a transcription regulator via its interaction with RARB
Catalytic activity
Pathway
Protein modification; protein ubiquitination.
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 876 | Glycyl thioester intermediate | ||||
Sequence: C |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | endoplasmic reticulum | |
Cellular Component | Golgi cisterna membrane | |
Cellular Component | Golgi membrane | |
Cellular Component | nuclear body | |
Cellular Component | nucleus | |
Molecular Function | small GTPase binding | |
Molecular Function | ubiquitin protein ligase activity | |
Molecular Function | ubiquitin-protein transferase activity | |
Biological Process | Golgi organization | |
Biological Process | membrane fusion | |
Biological Process | protein K48-linked ubiquitination | |
Biological Process | protein polyubiquitination | |
Biological Process | protein ubiquitination | |
Biological Process | regulation of cell migration | |
Biological Process | ubiquitin-dependent protein catabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameE3 ubiquitin-protein ligase HACE1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ3U0D9
- Secondary accessions
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: A significant portion localizes to the endoplasmic reticulum. Targeted to Golgi membrane via its interaction with Rab proteins (By similarity).
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Mice develop spontaneous, late-onset cancer. Moreover, tumor incidence in mice heterozygous for a p53/Tp53 mutation in higher in a Hace1-deficient background.
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 19 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000280623 | 1-909 | E3 ubiquitin-protein ligase HACE1 | |||
Sequence: MERAMEQLNRLTRSLRRARTVELPEDNETAVYTLMPMVMADQHRSVSELLSNSKFDVNYAFGRVKRSLLHIAANCGSVECLVLLLKKGANPNYQDISGCTPLHLAARNGQKKCMSKLLEYSADVNICNNEGLTAIHWLAVNGRTELLHDLVQHVTDVDVEDAMGQTALHVACQNGHKTTVQCLLDSGADINRPNVSGATPLYFACSHGQRDTAQILLLRGAKYLPDKNGVTPLDLCVQGGYGQTCEVLIQYHPRLFQTIVQMTQNEDLRENMLRQVLQHLSQQSESQYLKILTGLAEVATTNGHKLLSLSSNYDAQMKSLLRIVRIFCHVFRIGPSSPSNGIDMGYNGNKTPRSQVFKPLELLWHSLDEWLVLIATELMKNKEDSTDITSILLKQKGQDQEAPSLSAFEPPGPGSYESLPPGPGDSKPEVLAGEQEASADCQDVISVTANRLSAVIQAFYMCCSCQMPPGMTSPRFIEFVCKHDEVLKCFVNRNPKIIFDHFHFLLECPELMSRFMHIIKAQPFKDRCEWFYEHLHSGQPDSDMVHRPVSENDILLVHRDSIFRSSCEIVSKANCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVNDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA |
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Subunit
Interacts with RAB1 (RAB1A, RAB1B or RAB1C), RAB4 (RAB4A or RAB4B) and RAB11 (RAB11A or RAB11B); in a GTP-dependent manner. Interacts with RAC1; in a GTP-dependent manner. Interacts with the 26S proteasomal complex through the 20S core proteasomal subunit (By similarity).
Interacts with RARB
Interacts with RARB
Protein-protein interaction databases
Miscellaneous
Structure
Family & Domains
Features
Showing features for repeat, region, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Repeat | 64-93 | ANK 1 | ||||
Sequence: VKRSLLHIAANCGSVECLVLLLKKGANPNY | ||||||
Repeat | 97-126 | ANK 2 | ||||
Sequence: SGCTPLHLAARNGQKKCMSKLLEYSADVNI | ||||||
Repeat | 130-159 | ANK 3 | ||||
Sequence: EGLTAIHWLAVNGRTELLHDLVQHVTDVDV | ||||||
Repeat | 163-192 | ANK 4 | ||||
Sequence: MGQTALHVACQNGHKTTVQCLLDSGADINR | ||||||
Repeat | 196-226 | ANK 5 | ||||
Sequence: SGATPLYFACSHGQRDTAQILLLRGAKYLPD | ||||||
Repeat | 228-257 | ANK 6 | ||||
Sequence: NGVTPLDLCVQGGYGQTCEVLIQYHPRLFQ | ||||||
Region | 398-433 | Disordered | ||||
Sequence: QDQEAPSLSAFEPPGPGSYESLPPGPGDSKPEVLAG | ||||||
Domain | 574-909 | HECT | ||||
Sequence: NCAKLKQGIAVRFHGEEGMGQGVVREWFDILSNEIVNPDYALFTQSADGTTFQPNSNSYVNPDHLNYFRFAGQILGLALNHRQLVNIYFTRSFYKHILGIPVNYQDVASIDPEYAKNLQWILDNDISDLGLELTFSVETDVFGAMEEVPLKPGGGSILVTQNNKAEYVQLVTELRMTRAIQPQINAFLQGFHMFIPPSLIQLFDEYELELLLSGMPEIDVNDWIKNTEYTSGYEREDPVIQWFWEVVEDITQEERVLLLQFVTGSSRVPHGGFANIMGGSGLQNFTIAAVPYTPNLLPTSSTCINMLKLPEYPSKEILKDRLLVALHCGSYGYTMA |
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 4 isoforms produced by Alternative splicing.
Q3U0D9-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length909
- Mass (Da)102,114
- Last updated2005-10-11 v1
- Checksum804F62A06E9C78A0
Q3U0D9-2
- Name2
Q3U0D9-3
- Name3
- Differences from canonical
- 738-781: Missing
Q3U0D9-4
- Name4
Computationally mapped potential isoform sequences
There are 3 potential isoforms mapped to this entry
Sequence caution
Features
Showing features for sequence conflict, alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 92 | in Ref. 1; BAC31390 | ||||
Sequence: N → S | ||||||
Alternative sequence | VSP_023832 | 738-781 | in isoform 3 and isoform 4 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_042379 | 814-832 | in isoform 4 | |||
Sequence: Missing | ||||||
Alternative sequence | VSP_023833 | 838 | in isoform 2 | |||
Sequence: S → R | ||||||
Alternative sequence | VSP_023834 | 839-909 | in isoform 2 | |||
Sequence: Missing |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AK042879 EMBL· GenBank· DDBJ | BAC31390.1 EMBL· GenBank· DDBJ | mRNA | ||
AK156958 EMBL· GenBank· DDBJ | BAE33915.1 EMBL· GenBank· DDBJ | mRNA | ||
AC135669 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
AC153847 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. | |
BC025227 EMBL· GenBank· DDBJ | AAH25227.1 EMBL· GenBank· DDBJ | mRNA | ||
BC025474 EMBL· GenBank· DDBJ | AAH25474.1 EMBL· GenBank· DDBJ | mRNA | Different initiation | |
BC120695 EMBL· GenBank· DDBJ | AAI20696.1 EMBL· GenBank· DDBJ | mRNA | ||
BC120697 EMBL· GenBank· DDBJ | AAI20698.1 EMBL· GenBank· DDBJ | mRNA | ||
AK220383 EMBL· GenBank· DDBJ | BAD90440.1 EMBL· GenBank· DDBJ | mRNA |