Q3TIK0 · Q3TIK0_MOUSE
- ProteinPlatelet-activating factor acetylhydrolase IB subunit alpha
- GenePafah1b1
- StatusUniProtKB unreviewed (TrEMBL)
- Organism
- Amino acids410 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. Also required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet-activating factor (PAF) by removing the acetyl group at the SN-2 position.
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | axon cytoplasm | |
Cellular Component | centrosome | |
Cellular Component | cytoplasmic microtubule | |
Cellular Component | kinetochore | |
Cellular Component | microtubule associated complex | |
Cellular Component | neuronal cell body | |
Cellular Component | nuclear membrane | |
Cellular Component | spindle | |
Molecular Function | dynein complex binding | |
Molecular Function | microtubule plus-end binding | |
Biological Process | brain morphogenesis | |
Biological Process | cell division | |
Biological Process | establishment of mitotic spindle orientation | |
Biological Process | germ cell development | |
Biological Process | lipid catabolic process | |
Biological Process | microtubule organizing center organization | |
Biological Process | microtubule sliding | |
Biological Process | nuclear migration | |
Biological Process | retrograde axonal transport | |
Biological Process | vesicle transport along microtubule |
Keywords
- Molecular function
- Biological process
Names & Taxonomy
Protein names
- Recommended namePlatelet-activating factor acetylhydrolase IB subunit alpha
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ3TIK0
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: Localizes to the plus end of microtubules and to the centrosome. May localize to the nuclear membrane. Redistributes to axons during neuronal development. Also localizes to the microtubules of the manchette in elongating spermatids and to the meiotic spindle in spermatocytes.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for initiator methionine.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M |
Proteomic databases
PTM databases
Interaction
Subunit
Can self-associate. Interacts with DCX, dynein, dynactin, IQGAP1, KATNB1, NDE1, NDEL1, NUDC and RSN. Interacts with DISC1, and this interaction is enhanced by NDEL1. Interacts with DAB1 when DAB1 is phosphorylated in response to RELN/reelin signaling. Component of cytosolic PAF-AH IB, which is composed of PAFAH1B1 (alpha), PAFAH1B2 (beta) and PAFAH1B3 (gamma) subunits. Trimer formation is not essential for the catalytic activity of the enzyme which is contributed solely by the PAFAH1B2 (beta) and PAFAH1B3 (gamma) subunits.
Structure
Family & Domains
Features
Showing features for region, repeat.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-38 | Required for self-association and interaction with PAFAH1B2 and PAFAH1B3 | ||||
Sequence: MVLSQRQRDELNRAIADYLRSNGYEEAYSVFKKEAELD | ||||||
Region | 1-66 | Interaction with NDE1 | ||||
Sequence: MVLSQRQRDELNRAIADYLRSNGYEEAYSVFKKEAELDMNEELDKKYAGLLEKKWTSVIRLQKKVM | ||||||
Region | 1-102 | Interaction with NDEL1 | ||||
Sequence: MVLSQRQRDELNRAIADYLRSNGYEEAYSVFKKEAELDMNEELDKKYAGLLEKKWTSVIRLQKKVMELESKLNEAKEEFTSGGPLGQKRDPKEWIPRPPEKY | ||||||
Region | 83-410 | Interaction with dynein and dynactin | ||||
Sequence: GPLGQKRDPKEWIPRPPEKYALSGHRSPVTRVIFHPVFSVMVSASEDATIKVWDYETGDFERTLKGHTDSVQDISFDHSGKLLASCSADMTIKLWDFQGFECIRTMHGHDHNVSSVAIMPNGDHIVSASRDKTIKMWEVQTGYCVKTFTGHREWVRMVRPNQDGTLIASCSNDQTVRVWVVATKECKAELREHEHVVECISWAPESSYSSIYEATGSETKKSGKPGPFLLSGSRDKTIKMWDVSTGMCLMTLVGHDNWVRGVLFHSGGKFILSYADDKTLRVWDYKNKRCMKTLNAHEHFVTSLDFHKTAPYVVTGSVDQTVKVWECR | ||||||
Repeat | 104-145 | WD | ||||
Sequence: LSGHRSPVTRVIFHPVFSVMVSASEDATIKVWDYETGDFERT | ||||||
Repeat | 146-187 | WD | ||||
Sequence: LKGHTDSVQDISFDHSGKLLASCSADMTIKLWDFQGFECIRT | ||||||
Repeat | 188-229 | WD | ||||
Sequence: MHGHDHNVSSVAIMPNGDHIVSASRDKTIKMWEVQTGYCVKT | ||||||
Repeat | 230-271 | WD | ||||
Sequence: FTGHREWVRMVRPNQDGTLIASCSNDQTVRVWVVATKECKAE | ||||||
Repeat | 308-333 | WD | ||||
Sequence: GPFLLSGSRDKTIKMWDVSTGMCLMT | ||||||
Repeat | 334-375 | WD | ||||
Sequence: LVGHDNWVRGVLFHSGGKFILSYADDKTLRVWDYKNKRCMKT | ||||||
Repeat | 376-410 | WD | ||||
Sequence: LNAHEHFVTSLDFHKTAPYVVTGSVDQTVKVWECR | ||||||
Region | 388-410 | Interaction with NDEL1 | ||||
Sequence: FHKTAPYVVTGSVDQTVKVWECR |
Domain
Dimerization mediated by the LisH domain may be required to activate dynein.
Sequence similarities
Belongs to the WD repeat LIS1/nudF family.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length410
- Mass (Da)46,806
- Last updated2005-10-11 v1
- ChecksumBDA57A24A107F6C0