Q3TIK0 · Q3TIK0_MOUSE

  • Protein
    Platelet-activating factor acetylhydrolase IB subunit alpha
  • Gene
    Pafah1b1
  • Status
    UniProtKB unreviewed (TrEMBL)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    5/5

Function

function

Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. Also required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet-activating factor (PAF) by removing the acetyl group at the SN-2 position.

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentaxon cytoplasm
Cellular Componentcentrosome
Cellular Componentcytoplasmic microtubule
Cellular Componentkinetochore
Cellular Componentmicrotubule associated complex
Cellular Componentneuronal cell body
Cellular Componentnuclear membrane
Cellular Componentspindle
Molecular Functiondynein complex binding
Molecular Functionmicrotubule plus-end binding
Biological Processbrain morphogenesis
Biological Processcell division
Biological Processestablishment of mitotic spindle orientation
Biological Processgerm cell development
Biological Processlipid catabolic process
Biological Processmicrotubule organizing center organization
Biological Processmicrotubule sliding
Biological Processnuclear migration
Biological Processretrograde axonal transport
Biological Processvesicle transport along microtubule

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Platelet-activating factor acetylhydrolase IB subunit alpha
  • Alternative names
    • Lissencephaly-1 protein
      (LIS-1
      )
    • PAF acetylhydrolase 45 kDa subunit
      (PAF-AH 45 kDa subunit
      )
    • PAF-AH alpha
      (PAFAH alpha
      )

Gene names

    • Name
      Pafah1b1
    • Synonyms
      LIS1
      , PAFAH1B1

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • DBA/2
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus

Accessions

  • Primary accession
    Q3TIK0

Organism-specific databases

Subcellular Location

Cytoplasm, cytoskeleton
Nucleus membrane
Note: Localizes to the plus end of microtubules and to the centrosome. May localize to the nuclear membrane. Redistributes to axons during neuronal development. Also localizes to the microtubules of the manchette in elongating spermatids and to the meiotic spindle in spermatocytes.

Keywords

PTM/Processing

Features

Showing features for initiator methionine.

TypeIDPosition(s)Description
Initiator methionine1Removed

Proteomic databases

PTM databases

Interaction

Subunit

Can self-associate. Interacts with DCX, dynein, dynactin, IQGAP1, KATNB1, NDE1, NDEL1, NUDC and RSN. Interacts with DISC1, and this interaction is enhanced by NDEL1. Interacts with DAB1 when DAB1 is phosphorylated in response to RELN/reelin signaling. Component of cytosolic PAF-AH IB, which is composed of PAFAH1B1 (alpha), PAFAH1B2 (beta) and PAFAH1B3 (gamma) subunits. Trimer formation is not essential for the catalytic activity of the enzyme which is contributed solely by the PAFAH1B2 (beta) and PAFAH1B3 (gamma) subunits.

Family & Domains

Features

Showing features for region, repeat.

TypeIDPosition(s)Description
Region1-38Required for self-association and interaction with PAFAH1B2 and PAFAH1B3
Region1-66Interaction with NDE1
Region1-102Interaction with NDEL1
Region83-410Interaction with dynein and dynactin
Repeat104-145WD
Repeat146-187WD
Repeat188-229WD
Repeat230-271WD
Repeat308-333WD
Repeat334-375WD
Repeat376-410WD
Region388-410Interaction with NDEL1

Domain

Dimerization mediated by the LisH domain may be required to activate dynein.

Sequence similarities

Belongs to the WD repeat LIS1/nudF family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    410
  • Mass (Da)
    46,806
  • Last updated
    2005-10-11 v1
  • Checksum
    BDA57A24A107F6C0
MVLSQRQRDELNRAIADYLRSNGYEEAYSVFKKEAELDMNEELDKKYAGLLEKKWTSVIRLQKKVMELESKLNEAKEEFTSGGPLGQKRDPKEWIPRPPEKYALSGHRSPVTRVIFHPVFSVMVSASEDATIKVWDYETGDFERTLKGHTDSVQDISFDHSGKLLASCSADMTIKLWDFQGFECIRTMHGHDHNVSSVAIMPNGDHIVSASRDKTIKMWEVQTGYCVKTFTGHREWVRMVRPNQDGTLIASCSNDQTVRVWVVATKECKAELREHEHVVECISWAPESSYSSIYEATGSETKKSGKPGPFLLSGSRDKTIKMWDVSTGMCLMTLVGHDNWVRGVLFHSGGKFILSYADDKTLRVWDYKNKRCMKTLNAHEHFVTSLDFHKTAPYVVTGSVDQTVKVWECR

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AK167824
EMBL· GenBank· DDBJ
BAE39846.1
EMBL· GenBank· DDBJ
mRNA

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