Q3T9Z9 · ZUP1_MOUSE
- ProteinZinc finger-containing ubiquitin peptidase 1
- GeneZup1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids577 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Deubiquitinase with endodeubiquitinase activity that specifically interacts with and cleaves 'Lys-63'-linked long polyubiquitin chains. Shows only weak activity against 'Lys-11' and 'Lys-48'-linked chains. Plays an important role in genome stability pathways, functioning to prevent spontaneous DNA damage and also promote cellular survival in response to exogenous DNA damage. Modulates the ubiquitination status of replication protein A (RPA) complex proteins in response to replication stress.
Catalytic activity
Features
Showing features for active site, site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Active site | 359 | Nucleophile | |||
Site | 486 | Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole | |||
Active site | 490 | Proton acceptor | |||
Active site | 511 | ||||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | nucleoplasm | |
Molecular Function | cysteine-type deubiquitinase activity | |
Molecular Function | metal ion binding |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameZinc finger-containing ubiquitin peptidase 1
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ3T9Z9
- Secondary accessions
Proteomes
Organism-specific databases
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000244338 | 1-577 | Zinc finger-containing ubiquitin peptidase 1 | ||
Modified residue | 261 | N6-acetyllysine | |||
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Gene expression databases
Structure
Family & Domains
Features
Showing features for zinc finger, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Zinc finger | 2-24 | C2H2-type 1 | |||
Zinc finger | 29-52 | C2H2-type 2; atypical | |||
Region | 124-145 | Disordered | |||
Zinc finger | 153-176 | C2H2-type 3; atypical | |||
Zinc finger | 192-214 | C2H2-type 4 | |||
Region | 225-247 | MIU | |||
Region | 238-260 | Disordered | |||
Region | 248-273 | zUBD/ZHA | |||
Domain
The motif interacting with ubiquitin (MIU) and ZUFSP ubiquitin-binding domain (zUBD, also called ZUFSP helical arm ZHA) are responsible for binding the distal (outgoing) ubiquitin units S1 and S2 respectively.
C2H2-type zinc finger 4 is a ubiquitin-binding zinc finger (UBZ) and required for polyubiquitin binding, possibly binding the proximal ubiqutin, and for catalytic activity. C2H2-type zinc fingers 1-3 are required for localization to sites of DNA damage.
Sequence similarities
Belongs to the peptidase C78 family. ZUFSP subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoforms
- Sequence statusComplete
This entry describes 4 isoforms produced by Alternative splicing.
Q3T9Z9-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length577
- Mass (Da)65,593
- Last updated2006-06-27 v2
- Checksum711B6DE1629DFD01
Q3T9Z9-2
- Name2
- Differences from canonical
- 563-577: ARKQASQVFTAEKIP → VSIG
Q3T9Z9-3
- Name3
- Differences from canonical
- 529-562: Missing
Q3T9Z9-4
- Name4
Computationally mapped potential isoform sequences
There are 4 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1W2P6E9 | A0A1W2P6E9_MOUSE | Zup1 | 136 | ||
A0A1W2P7K8 | A0A1W2P7K8_MOUSE | Zup1 | 551 | ||
A0A1W2P761 | A0A1W2P761_MOUSE | Zup1 | 94 | ||
A0A1W2P714 | A0A1W2P714_MOUSE | Zup1 | 226 |
Sequence caution
Features
Showing features for sequence conflict, alternative sequence.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 5 | in Ref. 1; BAE42871 | |||
Sequence conflict | 50 | in Ref. 2; AAH89514 | |||
Sequence conflict | 182 | in Ref. 1; BAC36739 | |||
Alternative sequence | VSP_019534 | 264-279 | in isoform 4 | ||
Alternative sequence | VSP_019535 | 280-577 | in isoform 4 | ||
Alternative sequence | VSP_019536 | 529-562 | in isoform 3 | ||
Alternative sequence | VSP_019537 | 563-577 | in isoform 2 | ||
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AK012263 EMBL· GenBank· DDBJ | BAB28126.1 EMBL· GenBank· DDBJ | mRNA | ||
AK046191 EMBL· GenBank· DDBJ | BAC32628.1 EMBL· GenBank· DDBJ | mRNA | ||
AK052071 EMBL· GenBank· DDBJ | BAC34846.1 EMBL· GenBank· DDBJ | mRNA | ||
AK077304 EMBL· GenBank· DDBJ | BAC36739.1 EMBL· GenBank· DDBJ | mRNA | ||
AK077730 EMBL· GenBank· DDBJ | BAC36984.1 EMBL· GenBank· DDBJ | mRNA | Frameshift | |
AK172184 EMBL· GenBank· DDBJ | BAE42871.1 EMBL· GenBank· DDBJ | mRNA | ||
BC067065 EMBL· GenBank· DDBJ | AAH67065.1 EMBL· GenBank· DDBJ | mRNA | ||
BC089514 EMBL· GenBank· DDBJ | AAH89514.1 EMBL· GenBank· DDBJ | mRNA |