Q3T9E4 · TGTP2_MOUSE
- ProteinT-cell-specific guanine nucleotide triphosphate-binding protein 2
- GeneTgtp2
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids415 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Involved in innate cell-autonomous resistance to intracellular pathogens, such as Toxoplasma gondii (PubMed:19265156, PubMed:20109161, PubMed:24563254).
During avirulent type II T. gondii infection, recruited to the parasitophorous vacuole (PV) membrane, leading to PV vesiculation and rupture, and subsequent digestion of the parasite within the cytosol (PubMed:19265156, PubMed:24563254).
Not recruited to virulent type I T. gondii PV membrane (PubMed:19265156).
May confer an antiviral state for vesicular stomatitis virus (PubMed:9725230).
During avirulent type II T. gondii infection, recruited to the parasitophorous vacuole (PV) membrane, leading to PV vesiculation and rupture, and subsequent digestion of the parasite within the cytosol (PubMed:19265156, PubMed:24563254).
Not recruited to virulent type I T. gondii PV membrane (PubMed:19265156).
May confer an antiviral state for vesicular stomatitis virus (PubMed:9725230).
Catalytic activity
- GTP + H2O = GDP + H+ + phosphate
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 66 | GDP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 68 | GDP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 69 | GDP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 70 | GDP (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 90 | GDP (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 171 | GDP (UniProtKB | ChEBI) | ||||
Sequence: K | ||||||
Binding site | 173 | GDP (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 219 | GDP (UniProtKB | ChEBI) | ||||
Sequence: N |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | endoplasmic reticulum membrane | |
Cellular Component | Golgi apparatus | |
Molecular Function | GTP binding | |
Molecular Function | GTPase activity | |
Biological Process | autophagosome assembly | |
Biological Process | cellular response to interferon-beta | |
Biological Process | defense response to protozoan | |
Biological Process | innate immune response |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameT-cell-specific guanine nucleotide triphosphate-binding protein 2
- EC number
- Alternative names
Gene names
Organism names
- Organism
- Strains
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Mus > Mus
Accessions
- Primary accessionQ3T9E4
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Note: In astrocytes stimulated with IFNG or TNF, diffuse cytoplasmic localization decreases and the protein partially relocalizes to the endoplasmic reticulum and Golgi apparatus (PubMed:19285957).
Due to sequence similarity with Tgtp1, it is impossible to assign unambiguously experimental data published in the literature to Tgtp1 or Tgtp2 gene (Probable)
Due to sequence similarity with Tgtp1, it is impossible to assign unambiguously experimental data published in the literature to Tgtp1 or Tgtp2 gene (Probable)
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000437943 | 1-415 | T-cell-specific guanine nucleotide triphosphate-binding protein 2 | |||
Sequence: MAWASSFDAFFKNFKRESKIISEYDITLIMTYIEENKLQKAVSVIEKVLRDIESAPLHIAVTGETGAGKSTFINTLRGVGHEEKGAAPTGAIETTMKRTPYPHPKLPNVTIWDLPGIGTTNFTPQNYLTEMKFGEYDFFIIISATRFKENDAQLAKAIAQMGMNFYFVRTKIDSDLDNEQKFKPKSFNKEEVLKNIKDYCSNHLQESLDSEPPVFLVSNVDISKYDFPKLETKLLQDLPAHKRHVFSLSLQSLTEATINYKRDSLKQKVFLEAMKAGALATIPLGGMISDILENLDETFNLYRSYFGLDDASLENIAQDLNMSVDDFKVHLRFPHLFAEHNDESLEDKLFKYIKHISSVTGGPVAAVTYYRMAYYLQNLFLDTAANDAIALLNSKALFEKKVGPYISEPPEYWEA |
Proteomic databases
Expression
Tissue specificity
Expressed in thymus and lymph nodes, predominantly T-cells. Not expressed by immature CD4+ CD8+ thymocytes (at protein level) (PubMed:7836757).
Expressed in IFNG-stimulated macrophages (PubMed:7884320).
Expressed at low levels in unstimulated astrocytes (PubMed:19285957).
Due to sequence similarity with Tgtp1, it is impossible to assign unambiguously experimental data published in the literature to Tgtp1 or Tgtp2 gene
Expressed in IFNG-stimulated macrophages (PubMed:7884320).
Expressed at low levels in unstimulated astrocytes (PubMed:19285957).
Due to sequence similarity with Tgtp1, it is impossible to assign unambiguously experimental data published in the literature to Tgtp1 or Tgtp2 gene
Induction
In macrophages, up-regulated by IFNG, but not by IL2, IL4, IL10, nor TNF (PubMed:7884320).
Up-regulated by IFNG in lymph node cells and thymocytes and other cell types (PubMed:24563254, PubMed:7836757, PubMed:9725230).
In astrocytes, up-regulated by TNF and IFNG; when both cytokines are combined, the effect is synergistic (PubMed:19285957).
Due to sequence similarity with Tgtp1, it is impossible to assign unambiguously experimental data published in the literature to Tgtp1 or Tgtp2 gene (Probable)
Up-regulated by IFNG in lymph node cells and thymocytes and other cell types (PubMed:24563254, PubMed:7836757, PubMed:9725230).
In astrocytes, up-regulated by TNF and IFNG; when both cytokines are combined, the effect is synergistic (PubMed:19285957).
Due to sequence similarity with Tgtp1, it is impossible to assign unambiguously experimental data published in the literature to Tgtp1 or Tgtp2 gene (Probable)
Gene expression databases
Interaction
Miscellaneous
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 55-237 | IRG-type G | ||||
Sequence: APLHIAVTGETGAGKSTFINTLRGVGHEEKGAAPTGAIETTMKRTPYPHPKLPNVTIWDLPGIGTTNFTPQNYLTEMKFGEYDFFIIISATRFKENDAQLAKAIAQMGMNFYFVRTKIDSDLDNEQKFKPKSFNKEEVLKNIKDYCSNHLQESLDSEPPVFLVSNVDISKYDFPKLETKLLQD |
Sequence similarities
Belongs to the TRAFAC class dynamin-like GTPase superfamily. IRG family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length415
- Mass (Da)47,121
- Last updated2016-11-02 v2
- Checksum3AFB5F940242952A
Computationally mapped potential isoform sequences
There are 2 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1L1STI2 | A0A1L1STI2_MOUSE | Tgtp1 | 8 | ||
Q5SVP4 | Q5SVP4_MOUSE | Tgtp1 | 132 |
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 399 | in Ref. 2; BAE43078 | ||||
Sequence: E → G |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
L38444 EMBL· GenBank· DDBJ | AAA64914.1 EMBL· GenBank· DDBJ | mRNA | ||
AK088858 EMBL· GenBank· DDBJ | BAC40617.1 EMBL· GenBank· DDBJ | mRNA | ||
AK172580 EMBL· GenBank· DDBJ | BAE43078.1 EMBL· GenBank· DDBJ | mRNA | ||
FR734026 EMBL· GenBank· DDBJ | CBY65989.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AL645688 EMBL· GenBank· DDBJ | - | Genomic DNA | No translation available. |