Q3S2U1 · MOKE_MONPI

Function

function

Dehydrogenase; part of the gene cluster that mediates the biosynthesis of monakolin K, also known as lovastatin, and which acts as a potent competitive inhibitor of HMG-CoA reductase (PubMed:18578535).
Monakolin K biosynthesis is performed in two stages (PubMed:19693441).
The first stage is catalyzed by the nonaketide synthase mokA, which belongs to type I polyketide synthases and catalyzes the iterative nine-step formation of the polyketide (PubMed:18578535, PubMed:19693441).
This PKS stage is completed by the action of dehydrogenase mokE, which catalyzes the NADPH-dependent reduction of the unsaturated tetra-, penta- and heptaketide intermediates that arise during the mokA-mediated biosynthesis of the nonaketide chain and leads to dihydromonacolin L (PubMed:19693441).
Covalently bound dihydromonacolin L is released from mokA by the mokD esterase (By similarity).
Conversion of dihydromonacolin L into monacolin L and then monacolin J is subsequently performed with the participation of molecular oxygen and P450 monoogygenase mokC (PubMed:19693441).
Finally, mokF performs the conversion of monacoline J to monacoline K through the addition of the side-chain diketide moiety (2R)-2-methylbutanoate produced by the diketide synthase mokB (PubMed:19693441).

Biotechnology

Monacoline K acts as an inhibitor of HMG-CoA reductase involved in cholesterogenesis (PubMed:21821946).
Its hypocholesterolemic activity might be useful for lowering cholesterol levels in the blood and reduce artherosclerosis and coronary heart disease (PubMed:21821946).

Pathway

Polyketide biosynthesis; lovastatin biosynthesis.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site50-53NADP+ (UniProtKB | ChEBI)
Binding site134-141substrate
Binding site173-176NADP+ (UniProtKB | ChEBI)
Binding site196-199NADP+ (UniProtKB | ChEBI)
Binding site214NADP+ (UniProtKB | ChEBI)
Binding site261-262NADP+ (UniProtKB | ChEBI)
Binding site279NADP+ (UniProtKB | ChEBI)
Binding site281-285substrate
Binding site350-351NADP+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionnucleotide binding
Molecular Functionoxidoreductase activity, acting on NAD(P)H

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Dehydrogenase mokE
  • EC number
  • Alternative names
    • Enoyl reductase
    • Monacolin K biosynthesis protein E

Gene names

    • Name
      mokE

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Fungi > Dikarya > Ascomycota > Pezizomycotina > Eurotiomycetes > Eurotiomycetidae > Eurotiales > Aspergillaceae > Monascus

Accessions

  • Primary accession
    Q3S2U1

Phenotypes & Variants

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004362851-360Dehydrogenase mokE

Expression

Induction

Expression is controlled by the monacolin K cluster transcription regulator mokH (PubMed:19968298).

Interaction

Subunit

Monomer.

Structure

Family & Domains

Sequence similarities

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    360
  • Mass (Da)
    38,845
  • Last updated
    2005-10-11 v1
  • Checksum
    9C63B9ADD38679C7
MTITFTLPPHQTALTVDEHDKVTIWDVAPCPSLPPDQVCVRVEAVALNPSDTKMRGQFATPYAFLGTDYAGTVVAVGSQVTHIQVGDRVYGAQNEMCPRTPDQGAFSQYTVTRGRIWATVPEGWSFEQAASLPAGISTAGLAMKLLGLPLPDPNATTAPALPKPVYVLVYGGSTATATIVMQMLRLSGYIPIATCSPHNFDLAKKHGAEDVFDYRDAGLAQTIRTYTKNNLRYALDCITNVESTTLCFAAIGRAGGRYVSLNPFPEHAATRKMVTADWTLGPTIFGEGSTWPAPYGRPGSEKDRAFGEELWRVAARLVEDGKIVHHPLRVIPGGFEAIKQGMELVRTGQLSGEKVVVKLG

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
DQ176595
EMBL· GenBank· DDBJ
ABA02243.1
EMBL· GenBank· DDBJ
Genomic DNA

Similar Proteins

Disclaimer

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