Q3S2E4 · Q3S2E4_SARS

Function

function

Forms a primer, NSP9-pU, which is utilized by the polymerase for the initiation of RNA chains. Interacts with ribosome signal recognition particle RNA (SRP). Together with NSP8, suppress protein integration into the cell membrane, thereby disrupting host immune defenses.
Plays a role in viral RNA synthesis through two distinct activities. The N7-guanine methyltransferase activity plays a role in the formation of the cap structure GpppA-RNA. The proofreading exoribonuclease reduces the sensitivity of the virus to RNA mutagens during replication. This activity acts on both ssRNA and dsRNA in a 3'-5' direction.
RNA-directed RNA polymerase that catalyzes the transcription of viral genomic and subgenomic RNAs. Acts in complex with nsp7 and nsp8 to transcribe both the minus and positive strands of genomic RNA. The kinase-like NiRAN domain of NSP12 attaches one or more nucleotides to the amino terminus of NSP9, forming a covalent RNA-protein intermediate that serves as transcription/replication primer. Subgenomic RNAs (sgRNAs) are formed by discontinuous transcription: The polymerase has the ability to pause at transcription-regulating sequences (TRS) and jump to the leader TRS, resulting in a major deletion. This creates a series of subgenomic RNAs that are replicated, transcribed and translated. In addition, Nsp12 is a subunit of the viral RNA capping enzyme that catalyzes the RNA guanylyltransferase reaction for genomic and sub-genomic RNAs. Subsequently, the NiRAN domain transfers RNA to GDP, and forms the core cap structure GpppA-RNA.

Caution

Lacks conserved residue(s) required for the propagation of feature annotation.

Catalytic activity

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Features

Showing features for active site, binding site.

Type
IDPosition(s)Description
Active site5992
Active site5994
Active site6093
Active site6170
Active site6175
Binding site6233-6239S-adenosyl-L-methionine (UniProtKB | ChEBI)
Active site6663
Active site6678
Active site6718

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componenthost cell endoplasmic reticulum-Golgi intermediate compartment
Cellular Componenthost cell membrane
Cellular Componenthost cell perinuclear region of cytoplasm
Cellular Componentmembrane
Molecular Function3'-5'-RNA exonuclease activity
Molecular Function5'-3' DNA helicase activity
Molecular FunctionATP binding
Molecular Functioncysteine-type deubiquitinase activity
Molecular Functioncysteine-type endopeptidase activity
Molecular Functionendonuclease activity
Molecular FunctionG-quadruplex RNA binding
Molecular Functionlyase activity
Molecular FunctionmRNA (nucleoside-2'-O-)-methyltransferase activity
Molecular FunctionmRNA 5'-cap (guanine-N7-)-methyltransferase activity
Molecular Functionomega peptidase activity
Molecular FunctionRNA helicase activity
Molecular FunctionRNA-dependent RNA polymerase activity
Molecular Functionsingle-stranded RNA binding
Molecular Functionzinc ion binding
Biological ProcessDNA-templated transcription
Biological Processinduction by virus of host autophagy
Biological Processproteolysis
Biological Processsymbiont-mediated degradation of host mRNA
Biological Processsymbiont-mediated perturbation of host ubiquitin-like protein modification
Biological Processsymbiont-mediated suppression of host ISG15-protein conjugation
Biological Processsymbiont-mediated suppression of host NF-kappaB cascade
Biological Processsymbiont-mediated suppression of host type I interferon-mediated signaling pathway
Biological Processviral protein processing
Biological Processviral RNA genome replication
Biological Processviral translational frameshifting
Biological Processvirus-mediated perturbation of host defense response

Keywords

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    ORF1ab polyprotein

Organism names

  • Taxonomic identifier
  • Organism
  • Strain
    • ZJ0301
  • Taxonomic lineage
    Viruses > Riboviria > Orthornavirae > Pisuviricota > Pisoniviricetes > Nidovirales > Cornidovirineae > Coronaviridae > Orthocoronavirinae > Betacoronavirus > Sarbecovirus > Severe acute respiratory syndrome coronavirus

Accessions

  • Primary accession
    Q3S2E4

Proteomes

Subcellular Location

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane2206-2231Helical
Transmembrane2314-2333Helical
Transmembrane2340-2371Helical
Transmembrane2749-2768Helical
Transmembrane3020-3042Helical
Transmembrane3054-3078Helical
Transmembrane3115-3134Helical
Transmembrane3559-3582Helical
Transmembrane3588-3606Helical
Transmembrane3613-3634Helical
Transmembrane3660-3678Helical
Transmembrane3685-3701Helical
Transmembrane3721-3743Helical
Transmembrane3755-3774Helical

Keywords

PTM/Processing

Features

Showing features for disulfide bond.

TypeIDPosition(s)Description
Disulfide bond2259↔2265

Keywords

Interaction

Subunit

Interacts with nsp7 and nsp8 to form the replication-transcription complex (RTC): nsp12, nsp7, two subunits of nsp8, and up to two subunits of nsp13. Interacts with nsp9.

Family & Domains

Features

Showing features for domain, compositional bias, region.

Type
IDPosition(s)Description
Domain12-127CoV Nsp1 globular
Domain148-179BetaCoV Nsp1 C-terminal
Domain183-456CoV Nsp2 N-terminal
Domain458-688CoV Nsp2 middle
Domain690-818CoV Nsp2 C-terminal
Domain822-930Ubiquitin-like
Compositional bias972-997Acidic residues
Region972-1003Disordered
Domain1003-1169Macro
Region1175-1198Disordered
Domain1207-1335Macro
Domain1343-1470Macro
Domain1472-1538DPUP
Domain1542-1597Ubiquitin-like
Domain1611-1875Peptidase C16
Domain1888-1998Nucleic acid-binding
Domain2023-2132G2M
Domain2224-22943Ecto
Region2372-2462Y1
Domain2372-2740CoV Nsp3 Y
Region2376-2389ZF1
Region2422-2432ZF2
Region2463-2557Y2
Region2463-2740CoV-Y
Region2558-2639Y3
Region2640-2740Y4
Domain3142-3240Nsp4C
Domain3241-3546Peptidase C30
Domain3837-3919RdRp Nsp7 cofactor
Domain3920-4117RdRp Nsp8 cofactor
Domain4118-4230Nsp9 ssRNA-binding
Domain4231-4369ExoN/MTase coactivator
Domain4376-4630NiRAN
Domain4635-4733Nsp12 Interface
Domain4734-5301Nsp12 RNA-dependent RNA polymerase
Domain4981-5143RdRp catalytic
Domain5302-5385CV ZBD
Domain5558-5909+RNA virus helicase C-terminal
Domain5974-6189ExoN
Domain6198-6429N7-MTase
Region6316-6330GpppA-binding
Domain6430-6490Nsp15 N-terminal oligomerization
Domain6491-6616AV-Nsp11N/CoV-Nsp15M
Domain6633-6772NendoU
Domain6777-7071Nidovirus-type SAM-dependent 2'-O-MTase

Sequence similarities

Belongs to the coronaviruses polyprotein 1ab family.

Keywords

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    7,073
  • Mass (Da)
    790,257
  • Last updated
    2005-10-11 v1
  • Checksum
    96E95991DACA6EA9

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias972-997Acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
DQ182595
EMBL· GenBank· DDBJ
ABA02248.1
EMBL· GenBank· DDBJ
Genomic RNA

Similar Proteins

Disclaimer

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