Q3MSQ8 · DDX4_PELLE
- ProteinProbable ATP-dependent RNA helicase DDX4
- Geneddx4
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids724 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Probable ATP-dependent RNA helicase required during spermatogenesis to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle.
Catalytic activity
- ATP + H2O = ADP + H+ + phosphate
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | nucleus | |
Cellular Component | pi-body | |
Cellular Component | piP-body | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | RNA binding | |
Molecular Function | RNA helicase activity | |
Biological Process | male meiosis I | |
Biological Process | male meiotic nuclear division | |
Biological Process | oogenesis | |
Biological Process | piRNA processing | |
Biological Process | piRNA-mediated retrotransposon silencing by heterochromatin formation | |
Biological Process | retrotransposon silencing by piRNA-directed DNA methylation | |
Biological Process | spermatogenesis |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProbable ATP-dependent RNA helicase DDX4
- EC number
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Amphibia > Batrachia > Anura > Neobatrachia > Ranoidea > Ranidae > Pelophylax
Accessions
- Primary accessionQ3MSQ8
Subcellular Location
UniProt Annotation
GO Annotation
Note: Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis.
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000248852 | 1-724 | Probable ATP-dependent RNA helicase DDX4 | |||
Sequence: MSGQEDWESEIDNPPACVPNLSNSEPAFKASNQNYFSSNNAFNRTTERGFGNRKASDDCNQNFEFSERGFGKQRAGSDANQNFESSERGFGNRRGKGRGGFGTFGKDSNGKQESGDFTNDDNRTIDDNRRRGGFQRRGGFNDETSGRGRRGVRGGTSFSGFGREDGNEQSGFTSNDGFNNETSGFGSGRRGSRGDSSFSGDRESDRGRGFGRGGFRGRNEDIGVESGKGQEGFERSEQGPRVTYIPPPPPAEESDIFKHYQTGINFDKYDDIVVEVSGSDVPPAILTFEEANLCDSLAKNVCKSGYVKLTPIQKHSIPIIVAGRDLMACAQTGSGKTAAFLLPILAHLMVKGVESSAFQTLKEPEAIIVAPTRELINQIYLDARKFSYGTCVRPVVIYGGTQMFHSLKQISEGCNILCATPGRLLDVIRKEKIGLTKLRYLVLDEADRMLDMGFREDIENLLKSSGMPSKEERQTLMFSATFPSSIQSLAREILKPDYLFVVVGQVGGACSDVEQMVIEVDEFGKKDKLMEILQEIGSERTMVFVKTKKKADFIATFLCQEKVPSTSIHGDREQKERETALRDFRTGQCPVIVATSVAARGLDIENVSYVINFDIPDDIDEYVHRIGRTGRCGNTGRAISFFDKRGDDEQRIARSLVKVLSDAHQEVPAWLEEVAFSAHGSSAYNPRSNKFASTDDRKRGDSRGDYSTSGFSPSAAQAEEEDWG |
Structure
Family & Domains
Features
Showing features for region, compositional bias, motif, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-25 | Disordered | ||||
Sequence: MSGQEDWESEIDNPPACVPNLSNSE | ||||||
Compositional bias | 37-67 | Polar residues | ||||
Sequence: SSNNAFNRTTERGFGNRKASDDCNQNFEFSE | ||||||
Region | 37-241 | Disordered | ||||
Sequence: SSNNAFNRTTERGFGNRKASDDCNQNFEFSERGFGKQRAGSDANQNFESSERGFGNRRGKGRGGFGTFGKDSNGKQESGDFTNDDNRTIDDNRRRGGFQRRGGFNDETSGRGRRGVRGGTSFSGFGREDGNEQSGFTSNDGFNNETSGFGSGRRGSRGDSSFSGDRESDRGRGFGRGGFRGRNEDIGVESGKGQEGFERSEQGPR | ||||||
Compositional bias | 113-149 | Basic and acidic residues | ||||
Sequence: ESGDFTNDDNRTIDDNRRRGGFQRRGGFNDETSGRGR | ||||||
Compositional bias | 162-194 | Polar residues | ||||
Sequence: GREDGNEQSGFTSNDGFNNETSGFGSGRRGSRG | ||||||
Compositional bias | 195-235 | Basic and acidic residues | ||||
Sequence: DSSFSGDRESDRGRGFGRGGFRGRNEDIGVESGKGQEGFER | ||||||
Motif | 286-314 | Q motif | ||||
Sequence: LTFEEANLCDSLAKNVCKSGYVKLTPIQK | ||||||
Domain | 317-500 | Helicase ATP-binding | ||||
Sequence: IPIIVAGRDLMACAQTGSGKTAAFLLPILAHLMVKGVESSAFQTLKEPEAIIVAPTRELINQIYLDARKFSYGTCVRPVVIYGGTQMFHSLKQISEGCNILCATPGRLLDVIRKEKIGLTKLRYLVLDEADRMLDMGFREDIENLLKSSGMPSKEERQTLMFSATFPSSIQSLAREILKPDYLF | ||||||
Motif | 444-447 | DEAD box | ||||
Sequence: DEAD | ||||||
Domain | 512-675 | Helicase C-terminal | ||||
Sequence: DVEQMVIEVDEFGKKDKLMEILQEIGSERTMVFVKTKKKADFIATFLCQEKVPSTSIHGDREQKERETALRDFRTGQCPVIVATSVAARGLDIENVSYVINFDIPDDIDEYVHRIGRTGRCGNTGRAISFFDKRGDDEQRIARSLVKVLSDAHQEVPAWLEEVA | ||||||
Region | 683-724 | Disordered | ||||
Sequence: AYNPRSNKFASTDDRKRGDSRGDYSTSGFSPSAAQAEEEDWG |
Sequence similarities
Belongs to the DEAD box helicase family. DDX4/VASA subfamily.
Keywords
- Domain
Family and domain databases
Sequence
- Sequence statusComplete
- Length724
- Mass (Da)79,780
- Last updated2005-10-25 v1
- Checksum99DF0C59155F512C
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 37-67 | Polar residues | ||||
Sequence: SSNNAFNRTTERGFGNRKASDDCNQNFEFSE | ||||||
Compositional bias | 113-149 | Basic and acidic residues | ||||
Sequence: ESGDFTNDDNRTIDDNRRRGGFQRRGGFNDETSGRGR | ||||||
Compositional bias | 162-194 | Polar residues | ||||
Sequence: GREDGNEQSGFTSNDGFNNETSGFGSGRRGSRG | ||||||
Compositional bias | 195-235 | Basic and acidic residues | ||||
Sequence: DSSFSGDRESDRGRGFGRGGFRGRNEDIGVESGKGQEGFER |