Q3K425 · DNAA_STRA1

Function

function

Plays an essential role in the initiation and regulation of chromosomal replication. ATP-DnaA binds to the origin of replication (oriC) to initiate formation of the DNA replication initiation complex once per cell cycle. Binds the DnaA box (a 9 base pair repeat at the origin) and separates the double-stranded (ds)DNA. Forms a right-handed helical filament on oriC DNA; dsDNA binds to the exterior of the filament while single-stranded (ss)DNA is stabiized in the filament's interior. The ATP-DnaA-oriC complex binds and stabilizes one strand of the AT-rich DNA unwinding element (DUE), permitting loading of DNA polymerase. After initiation quickly degrades to an ADP-DnaA complex that is not apt for DNA replication. Binds acidic phospholipids.

Features

Showing features for binding site.

145350100150200250300350400450
TypeIDPosition(s)Description
Binding site157ATP (UniProtKB | ChEBI)
Binding site159ATP (UniProtKB | ChEBI)
Binding site160ATP (UniProtKB | ChEBI)
Binding site161ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentplasma membrane
Molecular FunctionATP binding
Molecular FunctionATP hydrolysis activity
Molecular FunctionDNA replication origin binding
Biological ProcessDNA replication initiation
Biological Processregulation of DNA replication

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Chromosomal replication initiator protein DnaA

Gene names

    • Name
      dnaA
    • Ordered locus names
      SAK_0001

Organism names

Accessions

  • Primary accession
    Q3K425

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_10000487371-453Chromosomal replication initiator protein DnaA

Interaction

Subunit

Oligomerizes as a right-handed, spiral filament on DNA at oriC.

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region1-78Domain I, interacts with DnaA modulators
Region78-112Domain II
Region113-331Domain III, AAA+ region
Region332-453Domain IV, binds dsDNA

Domain

Domain I is involved in oligomerization and binding regulators, domain II is flexibile and of varying length in different bacteria, domain III forms the AAA+ region, while domain IV binds dsDNA.

Sequence similarities

Belongs to the DnaA family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    453
  • Mass (Da)
    51,597
  • Last updated
    2005-11-08 v1
  • MD5 Checksum
    93E3EA68DFE968A539AB76EB39F28A1A
MTENEQLFWNRVLELSRSQIAPAAYEFFVLEARLLKIEHQTAVITLDNIEMKKLFWEQNLGPVILTAGFEIFNAEITANYVSNDLHLQETSFSNYQQSSNEVNTLPIRKIDSNLKEKYTFANFVQGDENRWAVSASIAVADSPGTTYNPLFIWGGPGLGKTHLLNAIGNQVLRDNPNARVLYITAENFINEFVSHIRLDSMEELKEKFRNLDLLLIDDIQSLAKKTLGGTQEEFFNTFNALHTNDKQIVLTSDRNPNQLNDLEERLVTRFSWGLPVNITPPDFETRVAILTNKIQEYPYDFPQDTIEYLAGEFDSNVRELEGALKNISLVADFKHAKTITVDIAAEAIRARKNDGPIVTVIPIEEIQIQVGKFYGVTVKEIKATKRTQDIVLARQVAMYLAREMTDNSLPKIGKEFGGRDHSTVLHAYNKIKNMVAQDDNLRIEIETIKNKIR

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000114
EMBL· GenBank· DDBJ
ABA45205.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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