Q3K425 · DNAA_STRA1
- ProteinChromosomal replication initiator protein DnaA
- GenednaA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids453 (go to sequence)
- Protein existenceInferred from homology
- Annotation score2/5
Function
function
Plays an essential role in the initiation and regulation of chromosomal replication. ATP-DnaA binds to the origin of replication (oriC) to initiate formation of the DNA replication initiation complex once per cell cycle. Binds the DnaA box (a 9 base pair repeat at the origin) and separates the double-stranded (ds)DNA. Forms a right-handed helical filament on oriC DNA; dsDNA binds to the exterior of the filament while single-stranded (ss)DNA is stabiized in the filament's interior. The ATP-DnaA-oriC complex binds and stabilizes one strand of the AT-rich DNA unwinding element (DUE), permitting loading of DNA polymerase. After initiation quickly degrades to an ADP-DnaA complex that is not apt for DNA replication. Binds acidic phospholipids.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 157 | ATP (UniProtKB | ChEBI) | |||
Binding site | 159 | ATP (UniProtKB | ChEBI) | |||
Binding site | 160 | ATP (UniProtKB | ChEBI) | |||
Binding site | 161 | ATP (UniProtKB | ChEBI) | |||
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytoplasm | |
Cellular Component | plasma membrane | |
Molecular Function | ATP binding | |
Molecular Function | ATP hydrolysis activity | |
Molecular Function | DNA replication origin binding | |
Biological Process | DNA replication initiation | |
Biological Process | regulation of DNA replication |
Keywords
- Molecular function
- Biological process
- Ligand
Names & Taxonomy
Protein names
- Recommended nameChromosomal replication initiator protein DnaA
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Bacillati > Bacillota > Bacilli > Lactobacillales > Streptococcaceae > Streptococcus
Accessions
- Primary accessionQ3K425
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_1000048737 | 1-453 | Chromosomal replication initiator protein DnaA | ||
Interaction
Subunit
Oligomerizes as a right-handed, spiral filament on DNA at oriC.
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-78 | Domain I, interacts with DnaA modulators | |||
Region | 78-112 | Domain II | |||
Region | 113-331 | Domain III, AAA+ region | |||
Region | 332-453 | Domain IV, binds dsDNA | |||
Domain
Domain I is involved in oligomerization and binding regulators, domain II is flexibile and of varying length in different bacteria, domain III forms the AAA+ region, while domain IV binds dsDNA.
Sequence similarities
Belongs to the DnaA family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length453
- Mass (Da)51,597
- Last updated2005-11-08 v1
- MD5 Checksum93E3EA68DFE968A539AB76EB39F28A1A
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000114 EMBL· GenBank· DDBJ | ABA45205.1 EMBL· GenBank· DDBJ | Genomic DNA |