Q3IHD2 · Q3IHD2_PSET1

Function

function

Endoribonuclease that plays a central role in RNA processing and decay. Required for the maturation of 5S and 16S rRNAs and the majority of tRNAs. Also involved in the degradation of most mRNAs.

Catalytic activity

  • Endonucleolytic cleavage of single-stranded RNA in A- and U-rich regions.
    EC:3.1.26.12 (UniProtKB | ENZYME | Rhea)

Cofactor

Protein has several cofactor binding sites:
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion per subunit.
Zn2+ (UniProtKB | Rhea| CHEBI:29105 )

Note: Binds 2 Zn2+ ions per homotetramer.

Features

Showing features for binding site.

110711002003004005006007008009001,000
TypeIDPosition(s)Description
Binding site302Mg2+ (UniProtKB | ChEBI); catalytic
Binding site345Mg2+ (UniProtKB | ChEBI); catalytic
Binding site403Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site406Zn2+ (UniProtKB | ChEBI); ligand shared between dimeric partners

GO annotations

AspectTerm
Cellular Componentcytoplasm
Cellular Componentcytoplasmic side of plasma membrane
Molecular Functionmagnesium ion binding
Molecular Functionribonuclease E activity
Molecular FunctionRNA endonuclease activity
Molecular FunctionrRNA binding
Molecular FunctiontRNA binding
Molecular Functionzinc ion binding
Biological ProcessmRNA catabolic process
Biological ProcessrRNA processing
Biological ProcesstRNA processing

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease E
  • EC number
  • Short names
    RNase E

Gene names

    • Name
      rne
    • Ordered locus names
      PSHAa1816

Organism names

Accessions

  • Primary accession
    Q3IHD2

Proteomes

Subcellular Location

Cytoplasm
Cell inner membrane
; Peripheral membrane protein

Keywords

Interaction

Subunit

Component of the RNA degradosome, which is a multiprotein complex involved in RNA processing and mRNA degradation. Within the RNA degradosome, RNase E assembles into a homotetramer formed by a dimer of dimers.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY Q3IHD2pnp Q3IJ732EBI-6412370, EBI-6412396

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain, region, compositional bias.

TypeIDPosition(s)Description
Domain39-119S1 motif
Region403-406Required for zinc-mediated homotetramerization and catalytic activity
Compositional bias572-601Basic and acidic residues
Region572-822Disordered
Compositional bias624-641Polar residues
Compositional bias658-696Basic and acidic residues
Compositional bias714-750Polar residues
Compositional bias751-768Basic and acidic residues
Region862-928Disordered
Region957-989Disordered
Region1011-1071Disordered
Compositional bias1032-1046Basic and acidic residues

Sequence similarities

Belongs to the RNase E/G family. RNase E subfamily.
Belongs to the RNase E/G family. RNase G subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    1,071
  • Mass (Da)
    118,803
  • Last updated
    2005-11-08 v1
  • Checksum
    10BF6A650606BBDA
MKRMLINATQQEEMRVALVDGQRLYDLDIESPGHEQKKANIYKGKITRIEPSLEAAFVEYGADRHGFLPLKEIARTYFPAGYTFHGRPNIRDVIKEGQEVIVQVDKEERGQKGAALTTFISVAGSYLVLMPNNPRAGGISRRIEGDERTELKESLSRLELPKGMGLIVRTAGVGKSFEELNYDLKALLVHWEAIGVAADSAKAPFLIHQESNVIFRAIRDYLRRDIGEILIDKPRVFEEAKAHIERFRPDFMSRVKLYQGDTPLFTHYQIESQIESAFQREVRLPSGGSIVIDPTEALTSIDINSSKATKGGDIEETALNTNLEAADEIARQLRLRDLGGLIVIDFIDMTPPRHQREVENRLKDAVRPDRARVQIGKISRFGLLEMSRQRLRPSLGEASQGPCPRCSGQGTIRSNESIALSILRLIEEEAIKDNTAQVNAQVPVAVAAYLLNEQRRSVHRMEKQHKCDIVIIPNQHMETPHYEVMRLRKDETIETVSYGQIVAPEPEAFEMSKSPVAPVREEPMLKGVVMPAAPAPQAAPANVVAPAETKAQSGLLDAIGKWFKSLFASETVEVKKEETQKQQQETRDNTRNNDNRRRNNNNQRRRNNPRSKPRTERTADEEVKTNSVSTPEAATQTNENQERGENRNKRRRNPNSRKRPEPRTEDKDAVKVDAPAKAEAETKPVQAEEPKDQKPKVRRQRRNLRKKVRLQDENAEQVQTTDETPVQATEKETPVAQEQAPVVEKQTQTAAEKTAHIKEEKVEDVDSNDETPTTEDEQEQTRTRSRRSPRHLRASGQRRRRPEGEGNEAKSDEAPAFVPVADQAAAEYEAELKAKSAATPADAAQQVEQAVAVEDLAKVEAVEEPVQTEEPAKVETPVVTEEPAKVETPVAAEEPAKVETPVAAEEPAKVEAPVVTEEPAKVETPLVTEEPAKVEAPVVTEEPAKVETPVVTEEPAKVEAPVVTEEPTKVETPVVTEEPTKVEAPVVTEEPAKVETPVVTAAPHVKTAIAQGSASAPMAQPTPVADSEVKHTSVAMAHDKRELVPDSGLRAGSIKPAGRASSTMTKTMSVD

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias572-601Basic and acidic residues
Compositional bias624-641Polar residues
Compositional bias658-696Basic and acidic residues
Compositional bias714-750Polar residues
Compositional bias751-768Basic and acidic residues
Compositional bias1032-1046Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CR954246
EMBL· GenBank· DDBJ
CAI86888.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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