Q3BCQ4 · RHCG_CANLF
- ProteinAmmonium transporter Rh type C
- GeneRHCG
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids460 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score4/5
Function
function
Ammonium transporter involved in the maintenance of acid-base homeostasis. Transports ammonium and its related derivative methylammonium across the plasma membrane of epithelial cells likely contributing to renal transepithelial ammonia transport and ammonia metabolism. Postulated to primarily mediate an electroneutral bidirectional transport of NH3 ammonia species according to a mechanism that implies interaction of an NH4+ ion with acidic residues of the pore entry followed by dissociation of NH4+ into NH3 and H+. As a result NH3 transits through the central pore and is protonated on the extracellular side reforming NH4+ (By similarity).
May act as a CO2 channel providing for renal acid secretion (By similarity).
May act as a CO2 channel providing for renal acid secretion (By similarity).
Catalytic activity
- NH4+(in) = NH4+(out)This reaction proceeds in the forward and the backward directions.
- methylamine(out) = methylamine(in)This reaction proceeds in the forward direction.
- CO2(out) = CO2(in)This reaction proceeds in the forward direction.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | apical plasma membrane | |
Cellular Component | basolateral plasma membrane | |
Cellular Component | cytoplasmic vesicle | |
Cellular Component | plasma membrane | |
Molecular Function | ammonium channel activity | |
Molecular Function | carbon dioxide transmembrane transporter activity | |
Biological Process | ammonium homeostasis | |
Biological Process | ammonium transmembrane transport |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameAmmonium transporter Rh type C
- Alternative names
Gene names
Organism names
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Carnivora > Caniformia > Canidae > Canis
Accessions
- Primary accessionQ3BCQ4
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Apical cell membrane ; Multi-pass membrane protein
Note: Also detected at the basolateral membrane and in subapical vesicles.
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 1-9 | Cytoplasmic | ||||
Sequence: MVWNTNLRW | ||||||
Transmembrane | 10-30 | Helical | ||||
Sequence: RLPVTCLLLQVALVVLFGVFV | ||||||
Topological domain | 31-61 | Extracellular | ||||
Sequence: RYDMDADPHWIDKKEAENSTSDMENEFYYRY | ||||||
Transmembrane | 62-82 | Helical | ||||
Sequence: PSFQDVHVMIFVGFGFLMTFL | ||||||
Topological domain | 83-90 | Cytoplasmic | ||||
Sequence: QRYGYSSV | ||||||
Transmembrane | 91-111 | Helical | ||||
Sequence: GFNFLLAAFGIQWALLLQGWF | ||||||
Topological domain | 112-125 | Extracellular | ||||
Sequence: HSYYRGYIRVGVEN | ||||||
Transmembrane | 126-145 | Helical | ||||
Sequence: LINADFCVGSVCVAFGAVLG | ||||||
Topological domain | 146-151 | Cytoplasmic | ||||
Sequence: KVSPVQ | ||||||
Transmembrane | 152-174 | Helical | ||||
Sequence: LLIMTLFQVTLFSVNEFILLNLL | ||||||
Topological domain | 175-179 | Extracellular | ||||
Sequence: EVKDA | ||||||
Transmembrane | 180-200 | Helical | ||||
Sequence: GGSMTIHTFGAYFGLTVTWIL | ||||||
Topological domain | 201-219 | Cytoplasmic | ||||
Sequence: YRPGLHQSKERQSSVYHSD | ||||||
Transmembrane | 220-240 | Helical | ||||
Sequence: LFAMIGTLFLWMYWPSFNSAV | ||||||
Topological domain | 241-251 | Extracellular | ||||
Sequence: SNHGDAQHRAA | ||||||
Transmembrane | 252-272 | Helical | ||||
Sequence: INTYCSLAACVLTSVALSSAL | ||||||
Topological domain | 273-285 | Cytoplasmic | ||||
Sequence: HKKGKLDMVHIQN | ||||||
Transmembrane | 286-306 | Helical | ||||
Sequence: ATLAGGVAVGTAAEMMLMPYG | ||||||
Topological domain | 307 | Extracellular | ||||
Sequence: S | ||||||
Transmembrane | 308-328 | Helical | ||||
Sequence: LIVGFICGIVSTLGFVYLTPF | ||||||
Topological domain | 329-339 | Cytoplasmic | ||||
Sequence: LESRLRVQDTC | ||||||
Transmembrane | 340-360 | Helical | ||||
Sequence: GIHNLHGIPGIIGAIVGAVTA | ||||||
Topological domain | 361-396 | Extracellular | ||||
Sequence: SCANTDVYGVNGLTQAFGFDGFKTNRTPSMQGKFQA | ||||||
Transmembrane | 397-417 | Helical | ||||
Sequence: AGLFVSLAMALVGGIIVGIIL | ||||||
Topological domain | 418-460 | Cytoplasmic | ||||
Sequence: KLPFWGQPADENCFEDAIYWEMPEEPKSTVLHPEDSTLKPSEP |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for chain, glycosylation.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000283576 | 1-460 | Ammonium transporter Rh type C | |||
Sequence: MVWNTNLRWRLPVTCLLLQVALVVLFGVFVRYDMDADPHWIDKKEAENSTSDMENEFYYRYPSFQDVHVMIFVGFGFLMTFLQRYGYSSVGFNFLLAAFGIQWALLLQGWFHSYYRGYIRVGVENLINADFCVGSVCVAFGAVLGKVSPVQLLIMTLFQVTLFSVNEFILLNLLEVKDAGGSMTIHTFGAYFGLTVTWILYRPGLHQSKERQSSVYHSDLFAMIGTLFLWMYWPSFNSAVSNHGDAQHRAAINTYCSLAACVLTSVALSSALHKKGKLDMVHIQNATLAGGVAVGTAAEMMLMPYGSLIVGFICGIVSTLGFVYLTPFLESRLRVQDTCGIHNLHGIPGIIGAIVGAVTASCANTDVYGVNGLTQAFGFDGFKTNRTPSMQGKFQAAGLFVSLAMALVGGIIVGIILKLPFWGQPADENCFEDAIYWEMPEEPKSTVLHPEDSTLKPSEP | ||||||
Glycosylation | 48 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N |
Post-translational modification
N-glycosylated.
Keywords
- PTM
Proteomic databases
PTM databases
Interaction
Structure
Family & Domains
Sequence similarities
Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length460
- Mass (Da)50,799
- Last updated2005-11-22 v1
- Checksum11D3BB0D9BE68841
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY831678 EMBL· GenBank· DDBJ | AAX39720.1 EMBL· GenBank· DDBJ | mRNA |