Q39099 · XTH4_ARATH

Function

function

Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.

Catalytic activity

  • breaks a beta-(1->4) bond in the backbone of a xyloglucan and transfers the xyloglucanyl segment on to O-4 of the non-reducing terminal glucose residue of an acceptor, which can be a xyloglucan or an oligosaccharide of xyloglucan.
    EC:2.4.1.207 (UniProtKB | ENZYME | Rhea)

Features

Showing features for active site, site, binding site.

129620406080100120140160180200220240260280
TypeIDPosition(s)Description
Active site109Nucleophile
Site111Important for catalytic activity
Active site113Proton donor
Binding site113xyloglucan (UniProtKB | ChEBI)
Binding site126-128xyloglucan (UniProtKB | ChEBI)
Binding site136-138xyloglucan (UniProtKB | ChEBI)
Binding site202-203xyloglucan (UniProtKB | ChEBI)
Binding site207xyloglucan (UniProtKB | ChEBI)
Binding site282xyloglucan (UniProtKB | ChEBI)

GO annotations

all annotationsall molecular functionnucleotide bindingmolecular_functionnucleic acid bindingdna bindingchromatin bindingdna-binding transcription factor activityrna bindingcytoskeletal motor activitycatalytic activitynuclease activitysignaling receptor bindingstructural molecule activitytransporter activitybindingprotein bindingtranslation factor activity, rna bindinglipid bindingkinase activitytransferase activityhydrolase activityoxygen bindingenzyme regulator activitycarbohydrate bindingsignaling receptor activitytranslation regulator activitytranscription regulator activityother molecular functionall biological processcarbohydrate metabolic processgeneration of precursor metabolites and energynucleobase-containing compound metabolic processdna metabolic processtranslationlipid metabolic processtransportresponse to stresscell cyclecell communicationsignal transductioncell-cell signalingmulticellular organism developmentcircadian rhythmbiological_processmetabolic processcatabolic processbiosynthetic processresponse to light stimulusresponse to external stimulustropismresponse to biotic stimulusresponse to abiotic stimulusresponse to endogenous stimulusembryo developmentpost-embryonic developmentfruit ripeningabscissionpollinationflower developmentcellular processprogrammed cell deathphotosynthesiscellular component organizationcell growthprotein metabolic processcellular homeostasissecondary metabolic processreproductive processcell differentiationprotein modification processgrowthepigenetic regulation of gene expressionresponse to chemicalanatomical structure developmentregulation of molecular functionother biological processall cellular componentcellular_componentextracellular regioncell wallintracellular anatomical structurenucleusnuclear envelopenucleoplasmnucleoluscytoplasmmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuscytosolribosomecytoskeletonplasma membranechloroplastplastidthylakoidmembraneexternal encapsulating structureother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentapoplast
Cellular Componentcytoplasm
Cellular Componentextracellular region
Cellular Componentmitochondrion
Cellular Componentplant-type cell wall
Cellular Componentplasmodesma
Molecular Functionhydrolase activity, hydrolyzing O-glycosyl compounds
Molecular Functionpolysaccharide binding
Molecular Functionxyloglucan:xyloglucosyl transferase activity
Biological Processcell wall organization
Biological Processplant-type secondary cell wall biogenesis
Biological Processresponse to auxin
Biological Processresponse to low light intensity stimulus
Biological Processresponse to mechanical stimulus
Biological Processunidimensional cell growth
Biological Processxyloglucan metabolic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Xyloglucan endotransglucosylase/hydrolase protein 4
  • EC number
  • Short names
    At-XTH4; XTH-4

Gene names

    • Name
      XTH4
    • Synonyms
      EXGT-A1, EXT
    • ORF names
      T9F8.4
    • Ordered locus names
      At2g06850

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q39099
  • Secondary accessions
    • Q94F33

Proteomes

Organism-specific databases

Genome annotation databases

PTM/Processing

Features

Showing features for signal, chain, glycosylation, disulfide bond.

TypeIDPosition(s)Description
Signal1-24
ChainPRO_000001180425-296Xyloglucan endotransglucosylase/hydrolase protein 4
Glycosylation117N-linked (GlcNAc...) asparagine
Disulfide bond231↔240
Disulfide bond277↔290

Post-translational modification

Contains at least one intrachain disulfide bond essential for its enzymatic activity.
N-glycosylated; not essential for its enzymatic activity.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Predominantly expressed in young developing tissues. Expressed in 7 day old seedlings, roots, nodes bearing flowers, flower buds and siliques.

Induction

By auxin and brassinolide.

Gene expression databases

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain26-223GH16

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.
  • Length
    296
  • Mass (Da)
    34,291
  • Last updated
    1996-11-01 v1
  • Checksum
    455F3574BE7751CC
MTVSSSPWALMALFLMVSSTMVMAIPPRKAIDVPFGRNYVPTWAFDHQKQFNGGSELQLILDKYTGTGFQSKGSYLFGHFSMHIKLPAGDTAGVVTAFYLSSTNNEHDEIDFEFLGNRTGQPAILQTNVFTGGKGNREQRIYLWFDPSKAYHTYSILWNMYQIVFFVDNIPIRTFKNAKDLGVRFPFNQPMKLYSSLWNADDWATRGGLEKTNWANAPFVASYKGFHIDGCQASVEAKYCATQGRMWWDQKEFRDLDAEQWRRLKWVRMKWTIYNYCTDRTRFPVMPAECKRDRDA

Computationally mapped potential isoform sequences

There is 1 potential isoform mapped to this entry

View all
EntryEntry nameGene nameLength
A0A1P8B288A0A1P8B288_ARATHXTH4236

Sequence caution

The sequence AAK62373.1 differs from that shown. Reason: Frameshift

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict205-206in Ref. 5; AAK62373

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
D16454
EMBL· GenBank· DDBJ
BAA03921.1
EMBL· GenBank· DDBJ
mRNA
AF163819
EMBL· GenBank· DDBJ
AAD45123.1
EMBL· GenBank· DDBJ
Genomic DNA
AC005561
EMBL· GenBank· DDBJ
AAC98464.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002685
EMBL· GenBank· DDBJ
AEC06021.1
EMBL· GenBank· DDBJ
Genomic DNA
AF386928
EMBL· GenBank· DDBJ
AAK62373.1
EMBL· GenBank· DDBJ
mRNA Frameshift
AY054547
EMBL· GenBank· DDBJ
AAK96738.1
EMBL· GenBank· DDBJ
mRNA
AY056201
EMBL· GenBank· DDBJ
AAL07050.1
EMBL· GenBank· DDBJ
mRNA
AY059873
EMBL· GenBank· DDBJ
AAL24355.1
EMBL· GenBank· DDBJ
mRNA
AY064672
EMBL· GenBank· DDBJ
AAL47378.1
EMBL· GenBank· DDBJ
mRNA
AY114644
EMBL· GenBank· DDBJ
AAM47963.1
EMBL· GenBank· DDBJ
mRNA
AY085465
EMBL· GenBank· DDBJ
AAM62691.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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