Q39099 · XTH4_ARATH
- ProteinXyloglucan endotransglucosylase/hydrolase protein 4
- GeneXTH4
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids296 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Catalyzes xyloglucan endohydrolysis (XEH) and/or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues.
Catalytic activity
Features
Showing features for active site, site, binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 109 | Nucleophile | ||||
Sequence: E | ||||||
Site | 111 | Important for catalytic activity | ||||
Sequence: D | ||||||
Active site | 113 | Proton donor | ||||
Sequence: E | ||||||
Binding site | 113 | xyloglucan (UniProtKB | ChEBI) | ||||
Sequence: E | ||||||
Binding site | 126-128 | xyloglucan (UniProtKB | ChEBI) | ||||
Sequence: QTN | ||||||
Binding site | 136-138 | xyloglucan (UniProtKB | ChEBI) | ||||
Sequence: NRE | ||||||
Binding site | 202-203 | xyloglucan (UniProtKB | ChEBI) | ||||
Sequence: DW | ||||||
Binding site | 207 | xyloglucan (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 282 | xyloglucan (UniProtKB | ChEBI) | ||||
Sequence: R |
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Cellular Component | apoplast | |
Cellular Component | cytoplasm | |
Cellular Component | extracellular region | |
Cellular Component | mitochondrion | |
Cellular Component | plant-type cell wall | |
Cellular Component | plasmodesma | |
Molecular Function | hydrolase activity, hydrolyzing O-glycosyl compounds | |
Molecular Function | polysaccharide binding | |
Molecular Function | xyloglucan:xyloglucosyl transferase activity | |
Biological Process | cell wall organization | |
Biological Process | plant-type secondary cell wall biogenesis | |
Biological Process | response to auxin | |
Biological Process | response to low light intensity stimulus | |
Biological Process | response to mechanical stimulus | |
Biological Process | unidimensional cell growth | |
Biological Process | xyloglucan metabolic process |
Keywords
- Molecular function
- Biological process
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameXyloglucan endotransglucosylase/hydrolase protein 4
- EC number
- Short namesAt-XTH4; XTH-4
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ39099
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Keywords
- Cellular component
PTM/Processing
Features
Showing features for signal, chain, glycosylation, disulfide bond.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-24 | |||||
Sequence: MTVSSSPWALMALFLMVSSTMVMA | ||||||
Chain | PRO_0000011804 | 25-296 | Xyloglucan endotransglucosylase/hydrolase protein 4 | |||
Sequence: IPPRKAIDVPFGRNYVPTWAFDHQKQFNGGSELQLILDKYTGTGFQSKGSYLFGHFSMHIKLPAGDTAGVVTAFYLSSTNNEHDEIDFEFLGNRTGQPAILQTNVFTGGKGNREQRIYLWFDPSKAYHTYSILWNMYQIVFFVDNIPIRTFKNAKDLGVRFPFNQPMKLYSSLWNADDWATRGGLEKTNWANAPFVASYKGFHIDGCQASVEAKYCATQGRMWWDQKEFRDLDAEQWRRLKWVRMKWTIYNYCTDRTRFPVMPAECKRDRDA | ||||||
Glycosylation | 117 | N-linked (GlcNAc...) asparagine | ||||
Sequence: N | ||||||
Disulfide bond | 231↔240 | |||||
Sequence: CQASVEAKYC | ||||||
Disulfide bond | 277↔290 | |||||
Sequence: CTDRTRFPVMPAEC |
Post-translational modification
Contains at least one intrachain disulfide bond essential for its enzymatic activity.
N-glycosylated; not essential for its enzymatic activity.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Predominantly expressed in young developing tissues. Expressed in 7 day old seedlings, roots, nodes bearing flowers, flower buds and siliques.
Induction
By auxin and brassinolide.
Gene expression databases
Structure
Family & Domains
Features
Showing features for domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 26-223 | GH16 | ||||
Sequence: PPRKAIDVPFGRNYVPTWAFDHQKQFNGGSELQLILDKYTGTGFQSKGSYLFGHFSMHIKLPAGDTAGVVTAFYLSSTNNEHDEIDFEFLGNRTGQPAILQTNVFTGGKGNREQRIYLWFDPSKAYHTYSILWNMYQIVFFVDNIPIRTFKNAKDLGVRFPFNQPMKLYSSLWNADDWATRGGLEKTNWANAPFVASY |
Sequence similarities
Belongs to the glycosyl hydrolase 16 family. XTH group 1 subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
- Length296
- Mass (Da)34,291
- Last updated1996-11-01 v1
- Checksum455F3574BE7751CC
Computationally mapped potential isoform sequences
There is 1 potential isoform mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A1P8B288 | A0A1P8B288_ARATH | XTH4 | 236 |
Sequence caution
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 205-206 | in Ref. 5; AAK62373 | ||||
Sequence: TR → PK |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
D16454 EMBL· GenBank· DDBJ | BAA03921.1 EMBL· GenBank· DDBJ | mRNA | ||
AF163819 EMBL· GenBank· DDBJ | AAD45123.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AC005561 EMBL· GenBank· DDBJ | AAC98464.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002685 EMBL· GenBank· DDBJ | AEC06021.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AF386928 EMBL· GenBank· DDBJ | AAK62373.1 EMBL· GenBank· DDBJ | mRNA | Frameshift | |
AY054547 EMBL· GenBank· DDBJ | AAK96738.1 EMBL· GenBank· DDBJ | mRNA | ||
AY056201 EMBL· GenBank· DDBJ | AAL07050.1 EMBL· GenBank· DDBJ | mRNA | ||
AY059873 EMBL· GenBank· DDBJ | AAL24355.1 EMBL· GenBank· DDBJ | mRNA | ||
AY064672 EMBL· GenBank· DDBJ | AAL47378.1 EMBL· GenBank· DDBJ | mRNA | ||
AY114644 EMBL· GenBank· DDBJ | AAM47963.1 EMBL· GenBank· DDBJ | mRNA | ||
AY085465 EMBL· GenBank· DDBJ | AAM62691.1 EMBL· GenBank· DDBJ | mRNA |