Q39085 · DIM_ARATH
- ProteinDelta(24)-sterol reductase
- GeneDIM
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids561 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
Plays a critical role in the general process of plant cell elongation. Involved in the synthesis of campesterol, an early precursor of brassinolide. Required for the conversion of 24-methylenecholesterol to campesterol and for the conversion of isofucosterol to sitosterol. Necessary for both the isomerization and reduction of 24-methylenecholesterol. Regulates indirectly phytochrome-mediated light responses through the modulation of brassinosteroid biosynthesis.
Catalytic activity
- lathosterol + NADP+ = 5alpha-cholesta-7,24-dien-3beta-ol + NADPH + H+
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | endoplasmic reticulum | |
Cellular Component | membrane | |
Cellular Component | plant-type vacuole | |
Cellular Component | vacuole | |
Molecular Function | calmodulin binding | |
Molecular Function | delta24-sterol reductase activity | |
Molecular Function | FAD binding | |
Biological Process | brassinosteroid biosynthetic process | |
Biological Process | lignin metabolic process | |
Biological Process | plant-type secondary cell wall biogenesis | |
Biological Process | unidimensional cell growth |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameDelta(24)-sterol reductase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ39085
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Microsome membrane ; Single-pass type II membrane protein
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Topological domain | 1-25 | Lumenal | |||
Transmembrane | 26-46 | Helical; Signal-anchor | |||
Topological domain | 47-561 | Cytoplasmic | |||
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Dwarf and reduced fertility.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Mutagenesis | 131 | In dwf1-9; dwarf phenotype. | |||
Mutagenesis | 163 | In dwf1-3; dwarf phenotype. | |||
Mutagenesis | 172 | In dwf1-10; dwarf phenotype. | |||
Mutagenesis | 220 | In dwf1-11; dwarf phenotype. | |||
Mutagenesis | 282 | In dwf1-7; dwarf phenotype. | |||
Mutagenesis | 521-523 | Loss of calmodulin binding and loss of function. | |||
Mutagenesis | 531 | Decreased calmodulin binding and partial loss of function. | |||
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 5 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for chain, modified residue.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Chain | PRO_0000219495 | 1-561 | Delta24-sterol reductase | ||
Modified residue | 2 | Phosphoserine | |||
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Subunit
Interacts with calmodulin.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | IntAct | |
---|---|---|---|---|---|
BINARY | Q39085 | At3g54130 Q9M391 | 3 | EBI-16906963, EBI-25520805 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Domain | 49-232 | FAD-binding PCMH-type | |||
Region | 518-539 | Interaction with calmodulin | |||
Sequence similarities
Belongs to the DIMINUTO family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length561
- Mass (Da)65,394
- Last updated2001-11-02 v2
- ChecksumCD25EF655AD44200
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Sequence conflict | 399 | in Ref. 5; AAL91175/AAN15686 | |||
Sequence conflict | 450 | in Ref. 1; AAA67055 | |||
Sequence conflict | 476 | in Ref. 1; AAA67055 | |||
Sequence conflict | 506 | in Ref. 1; AAA67055 | |||
Sequence conflict | 527 | in Ref. 5; AAL91175/AAN15686 | |||
Sequence conflict | 556-558 | in Ref. 1; AAA67055 | |||
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
L38520 EMBL· GenBank· DDBJ | AAA67055.1 EMBL· GenBank· DDBJ | mRNA | ||
U12400 EMBL· GenBank· DDBJ | AAA20244.1 EMBL· GenBank· DDBJ | mRNA | ||
AB025631 EMBL· GenBank· DDBJ | BAB01296.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | AEE76293.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | AEE76294.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002686 EMBL· GenBank· DDBJ | AEE76295.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY072216 EMBL· GenBank· DDBJ | AAL60037.1 EMBL· GenBank· DDBJ | mRNA | ||
AY081286 EMBL· GenBank· DDBJ | AAL91175.1 EMBL· GenBank· DDBJ | mRNA | ||
AY096472 EMBL· GenBank· DDBJ | AAM20112.1 EMBL· GenBank· DDBJ | mRNA | ||
BT000367 EMBL· GenBank· DDBJ | AAN15686.1 EMBL· GenBank· DDBJ | mRNA | ||
AK226335 EMBL· GenBank· DDBJ | BAE98484.1 EMBL· GenBank· DDBJ | mRNA | ||
AB493623 EMBL· GenBank· DDBJ | BAH30461.1 EMBL· GenBank· DDBJ | mRNA |