Q38906 · TOC34_ARATH

Function

function

GTPase involved in protein precursor import into chloroplasts. Seems to recognize chloroplast-destined precursor proteins and regulate their presentation to the translocation channel through GTP hydrolysis. Probably specialized in the import of nuclear encoded non-photosynthetic preproteins from the cytoplasm to the chloroplast.

Cofactor

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Binds 1 Mg2+ ion by subunit.

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
130 nMGDP7.4
6.3 μMGTP7.625
Vmax pH TEMPERATURE[C] NOTES EVIDENCE
940 nmol/min/ug7.4with GTP as substrate

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site48-53GTP (UniProtKB | ChEBI)
Binding site52Mg2+ (UniProtKB | ChEBI)
Binding site67-72GTP (UniProtKB | ChEBI)
Binding site70Mg2+ (UniProtKB | ChEBI)
Binding site162GTP (UniProtKB | ChEBI)
Binding site210-211GTP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentchloroplast
Cellular Componentchloroplast envelope
Cellular Componentchloroplast outer membrane
Cellular Componentplasma membrane
Cellular Componentplastid
Molecular FunctionGTP binding
Molecular FunctionGTPase activity
Molecular Functionidentical protein binding
Molecular Functionmetal ion binding
Molecular Functionprotein-transporting ATPase activity
Biological Processchloroplast localization
Biological Processintracellular protein transport
Biological Processprotein targeting to chloroplast

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Translocase of chloroplast 34, chloroplastic
  • EC number
  • Short names
    AtToc34
  • Alternative names
    • 34 kDa chloroplast outer envelope protein
    • GTP-binding protein OEP34
    • Plastid protein import 3

Gene names

    • Name
      TOC34
    • Synonyms
      OEP34, PPI3
    • ORF names
      MUG13.14
    • Ordered locus names
      At5g05000

Organism names

  • Taxonomic identifier
  • Strain
    • cv. Columbia
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q38906
  • Secondary accessions
    • Q0WNL4
    • Q8L9G5
    • Q9FF74
    • Q9GDD2

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Plastid, chloroplast outer membrane
; Single-pass membrane protein
Note: May contain beta barrel transmembrane regions.

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane269-286Helical

Keywords

PTM/Processing

Features

Showing features for initiator methionine, modified residue, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
Modified residue2N-acetylalanine
ChainPRO_00001447922-313Translocase of chloroplast 34, chloroplastic

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Mostly expressed in roots and flowers, and, to a lower extent, in seedlings, stems, and leaves.

Induction

Induced in light but repressed in darkness.

Developmental stage

Expressed at a relatively uniform, low level at most stages of development. Observed in cotyledons and hypocotyls of young seedling. Expressed in apical meristems. Found in the whole roots. Expressed in middle-aged leaves. In flowers, mostly localized in meristems, but is also present in all organs.

Gene expression databases

Interaction

Subunit

Homodimer, heterodimer with TOC33, and monomer. Part of the TOC core complex that includes 1 protein for the specific recognition of transit peptides surrounded by a ring composed of four proteins forming translocation channels, and four to five GTP-binding proteins providing energy. This core complex can interact with components of the TIC complex to form a larger import complex. Chloroplastic protein precursor such as prSS (precursor of the RuBisCO small subunit) interacts with these complexes. The TOC complex contains a specific subset of polar lipids such as digalactosyldiacylglyceride (DGDG), phosphatidylcholine (PC) and phosphatidylglycerol (PG). Interacts at least with TOC75. Interacts with AKR2A and AKR2B (PubMed:20215589).

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
XENO Q38906RBCS P692496EBI-1766808, EBI-1766821
BINARY Q38906TOC159 O812835EBI-1766808, EBI-639063
BINARY Q38906TOC34 Q389062EBI-1766808, EBI-1766808

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain, region, motif.

TypeIDPosition(s)Description
Domain36-260AIG1-type G
Region45-52G1
Region67-70Homodimerization
Region71-75G2
Region92-95G3
Region127-132Homodimerization
Region161-164G4
Region210-212G5
Motif287-295AKR2A-binding sequence (ABS) required for chloroplast outer envelope membrane targeting

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    313
  • Mass (Da)
    34,707
  • Last updated
    2008-02-05 v2
  • Checksum
    1C90E435B2EE0441
MAALQTLREWIGIQQFPPATQSKLLEILGKYKEEDVSSLTVLVMGKGGVGKSSTVNSVIGEKAAAVSTFQSEGLRPTLVSRTRSGFTLNIIDTPGLIEGGYVNDQAINIIKRFLLNMTIDVLLYVDRLDVYRVDDLDRQVVGAITDAFGKEIWKKSALVLTHAQFSPPDGLNYNHFVSKRSNALLKVIQTGAQLKKQDLQGFSIPVILVENSGRCHKNESDEKILPCGTSWIPNLFNKITEISFNGNKAIHVDKKLVEGPNPNERGKKLIPLMFAFQYLLVMKPLVRAIKSDVSRESKPAWELRDSGLASRRS

Features

Showing features for sequence conflict.

TypeIDPosition(s)Description
Sequence conflict44in Ref. 6; AAM65983
Sequence conflict47in Ref. 6; AAM65983
Sequence conflict299in Ref. 1; AAD09203 and 2; CAC17699

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
U43377
EMBL· GenBank· DDBJ
AAD09203.1
EMBL· GenBank· DDBJ
mRNA
AJ132696
EMBL· GenBank· DDBJ
CAC17699.1
EMBL· GenBank· DDBJ
mRNA
AB005245
EMBL· GenBank· DDBJ
BAB11522.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002688
EMBL· GenBank· DDBJ
AED90814.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002688
EMBL· GenBank· DDBJ
AED90815.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002688
EMBL· GenBank· DDBJ
AED90816.1
EMBL· GenBank· DDBJ
Genomic DNA
AK229425
EMBL· GenBank· DDBJ
BAF01285.1
EMBL· GenBank· DDBJ
mRNA
AY088447
EMBL· GenBank· DDBJ
AAM65983.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

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