Q38906 · TOC34_ARATH
- ProteinTranslocase of chloroplast 34, chloroplastic
- GeneTOC34
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids313 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
GTPase involved in protein precursor import into chloroplasts. Seems to recognize chloroplast-destined precursor proteins and regulate their presentation to the translocation channel through GTP hydrolysis. Probably specialized in the import of nuclear encoded non-photosynthetic preproteins from the cytoplasm to the chloroplast.
Cofactor
Note: Binds 1 Mg2+ ion by subunit.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
130 nM | GDP | 7.4 | ||||
6.3 μM | GTP | 7.6 | 25 |
Vmax | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|
940 nmol/min/ug | 7.4 | with GTP as substrate |
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 48-53 | GTP (UniProtKB | ChEBI) | ||||
Sequence: GVGKSS | ||||||
Binding site | 52 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: S | ||||||
Binding site | 67-72 | GTP (UniProtKB | ChEBI) | ||||
Sequence: STFQSE | ||||||
Binding site | 70 | Mg2+ (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 162 | GTP (UniProtKB | ChEBI) | ||||
Sequence: H | ||||||
Binding site | 210-211 | GTP (UniProtKB | ChEBI) | ||||
Sequence: EN |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Cellular Component | chloroplast envelope | |
Cellular Component | chloroplast outer membrane | |
Cellular Component | plasma membrane | |
Cellular Component | plastid | |
Molecular Function | GTP binding | |
Molecular Function | GTPase activity | |
Molecular Function | identical protein binding | |
Molecular Function | metal ion binding | |
Molecular Function | protein-transporting ATPase activity | |
Biological Process | chloroplast localization | |
Biological Process | intracellular protein transport | |
Biological Process | protein targeting to chloroplast |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameTranslocase of chloroplast 34, chloroplastic
- EC number
- Short namesAtToc34
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ38906
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Plastid, chloroplast outer membrane ; Single-pass membrane protein
Note: May contain beta barrel transmembrane regions.
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 269-286 | Helical | ||||
Sequence: LIPLMFAFQYLLVMKPLV |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for initiator methionine, modified residue, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Modified residue | 2 | N-acetylalanine | ||||
Sequence: A | ||||||
Chain | PRO_0000144792 | 2-313 | Translocase of chloroplast 34, chloroplastic | |||
Sequence: AALQTLREWIGIQQFPPATQSKLLEILGKYKEEDVSSLTVLVMGKGGVGKSSTVNSVIGEKAAAVSTFQSEGLRPTLVSRTRSGFTLNIIDTPGLIEGGYVNDQAINIIKRFLLNMTIDVLLYVDRLDVYRVDDLDRQVVGAITDAFGKEIWKKSALVLTHAQFSPPDGLNYNHFVSKRSNALLKVIQTGAQLKKQDLQGFSIPVILVENSGRCHKNESDEKILPCGTSWIPNLFNKITEISFNGNKAIHVDKKLVEGPNPNERGKKLIPLMFAFQYLLVMKPLVRAIKSDVSRESKPAWELRDSGLASRRS |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Mostly expressed in roots and flowers, and, to a lower extent, in seedlings, stems, and leaves.
Induction
Induced in light but repressed in darkness.
Developmental stage
Expressed at a relatively uniform, low level at most stages of development. Observed in cotyledons and hypocotyls of young seedling. Expressed in apical meristems. Found in the whole roots. Expressed in middle-aged leaves. In flowers, mostly localized in meristems, but is also present in all organs.
Gene expression databases
Interaction
Subunit
Homodimer, heterodimer with TOC33, and monomer. Part of the TOC core complex that includes 1 protein for the specific recognition of transit peptides surrounded by a ring composed of four proteins forming translocation channels, and four to five GTP-binding proteins providing energy. This core complex can interact with components of the TIC complex to form a larger import complex. Chloroplastic protein precursor such as prSS (precursor of the RuBisCO small subunit) interacts with these complexes. The TOC complex contains a specific subset of polar lipids such as digalactosyldiacylglyceride (DGDG), phosphatidylcholine (PC) and phosphatidylglycerol (PG). Interacts at least with TOC75. Interacts with AKR2A and AKR2B (PubMed:20215589).
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
XENO | Q38906 | RBCS P69249 | 6 | EBI-1766808, EBI-1766821 | |
BINARY | Q38906 | TOC159 O81283 | 5 | EBI-1766808, EBI-639063 | |
BINARY | Q38906 | TOC34 Q38906 | 2 | EBI-1766808, EBI-1766808 |
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, region, motif.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 36-260 | AIG1-type G | ||||
Sequence: VSSLTVLVMGKGGVGKSSTVNSVIGEKAAAVSTFQSEGLRPTLVSRTRSGFTLNIIDTPGLIEGGYVNDQAINIIKRFLLNMTIDVLLYVDRLDVYRVDDLDRQVVGAITDAFGKEIWKKSALVLTHAQFSPPDGLNYNHFVSKRSNALLKVIQTGAQLKKQDLQGFSIPVILVENSGRCHKNESDEKILPCGTSWIPNLFNKITEISFNGNKAIHVDKKLVEGP | ||||||
Region | 45-52 | G1 | ||||
Sequence: GKGGVGKS | ||||||
Region | 67-70 | Homodimerization | ||||
Sequence: STFQ | ||||||
Region | 71-75 | G2 | ||||
Sequence: SEGLR | ||||||
Region | 92-95 | G3 | ||||
Sequence: DTPG | ||||||
Region | 127-132 | Homodimerization | ||||
Sequence: RLDVYR | ||||||
Region | 161-164 | G4 | ||||
Sequence: THAQ | ||||||
Region | 210-212 | G5 | ||||
Sequence: ENS | ||||||
Motif | 287-295 | AKR2A-binding sequence (ABS) required for chloroplast outer envelope membrane targeting | ||||
Sequence: RAIKSDVSR |
Sequence similarities
Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. AIG1/Toc34/Toc159-like paraseptin GTPase family. TOC34 subfamily.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length313
- Mass (Da)34,707
- Last updated2008-02-05 v2
- Checksum1C90E435B2EE0441
Features
Showing features for sequence conflict.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Sequence conflict | 44 | in Ref. 6; AAM65983 | ||||
Sequence: M → I | ||||||
Sequence conflict | 47 | in Ref. 6; AAM65983 | ||||
Sequence: G → S | ||||||
Sequence conflict | 299 | in Ref. 1; AAD09203 and 2; CAC17699 | ||||
Sequence: P → L |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U43377 EMBL· GenBank· DDBJ | AAD09203.1 EMBL· GenBank· DDBJ | mRNA | ||
AJ132696 EMBL· GenBank· DDBJ | CAC17699.1 EMBL· GenBank· DDBJ | mRNA | ||
AB005245 EMBL· GenBank· DDBJ | BAB11522.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002688 EMBL· GenBank· DDBJ | AED90814.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002688 EMBL· GenBank· DDBJ | AED90815.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002688 EMBL· GenBank· DDBJ | AED90816.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AK229425 EMBL· GenBank· DDBJ | BAF01285.1 EMBL· GenBank· DDBJ | mRNA | ||
AY088447 EMBL· GenBank· DDBJ | AAM65983.1 EMBL· GenBank· DDBJ | mRNA |