Q38856 · IRT1_ARATH
- ProteinFe(2+) transport protein 1
- GeneIRT1
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids347 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
High-affinity iron transporter that plays a key role in the uptake of iron from the rhizosphere across the plasma membrane in the root epidermal layer. Acts as the principal regulator of iron homeostasis in planta. Also mediates the heavy metals uptake under iron-deficiency by its ability to transport cobalt, cadmium, manganese and/or zinc ions.
Miscellaneous
Inhibited by cadmium and Fe2+ ions and at 100-fold excess inhibited by cobalt, manganese and zinc ions.
The availability of secondary non-iron metal substrates (Zn, Mn, and Co) controls the localization of IRT1 between the outer polar domain of the plasma membrane and early endosome/trans-Golgi network in root epidermal cells.
GO annotations
all annotations | all molecular function | nucleotide binding | molecular_function | nucleic acid binding | dna binding | chromatin binding | dna-binding transcription factor activity | rna binding | cytoskeletal motor activity | catalytic activity | nuclease activity | signaling receptor binding | structural molecule activity | transporter activity | binding | protein binding | translation factor activity, rna binding | lipid binding | kinase activity | transferase activity | hydrolase activity | oxygen binding | enzyme regulator activity | carbohydrate binding | signaling receptor activity | translation regulator activity | transcription regulator activity | other molecular function | all biological process | carbohydrate metabolic process | generation of precursor metabolites and energy | nucleobase-containing compound metabolic process | dna metabolic process | translation | lipid metabolic process | transport | response to stress | cell cycle | cell communication | signal transduction | cell-cell signaling | multicellular organism development | circadian rhythm | biological_process | metabolic process | catabolic process | biosynthetic process | response to light stimulus | response to external stimulus | tropism | response to biotic stimulus | response to abiotic stimulus | response to endogenous stimulus | embryo development | post-embryonic development | fruit ripening | abscission | pollination | flower development | cellular process | programmed cell death | photosynthesis | cellular component organization | cell growth | protein metabolic process | cellular homeostasis | secondary metabolic process | reproductive process | cell differentiation | protein modification process | growth | epigenetic regulation of gene expression | response to chemical | anatomical structure development | regulation of molecular function | other biological process | all cellular component | cellular_component | extracellular region | cell wall | intracellular anatomical structure | nucleus | nuclear envelope | nucleoplasm | nucleolus | cytoplasm | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | cytosol | ribosome | cytoskeleton | plasma membrane | chloroplast | plastid | thylakoid | membrane | external encapsulating structure | other cellular component | |||
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Aspect | Term | |
---|---|---|
Cellular Component | early endosome | |
Cellular Component | endosome | |
Cellular Component | plasma membrane | |
Cellular Component | trans-Golgi network | |
Cellular Component | vacuole | |
Molecular Function | copper ion transmembrane transporter activity | |
Molecular Function | iron ion transmembrane transporter activity | |
Molecular Function | zinc ion transmembrane transporter activity | |
Biological Process | metal ion transport | |
Biological Process | nickel cation transport | |
Biological Process | response to bacterium |
Keywords
- Biological process
- Ligand
Enzyme and pathway databases
Protein family/group databases
Names & Taxonomy
Protein names
- Recommended nameFe(2+) transport protein 1
- Alternative names
Gene names
Organism names
- Strains
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis
Accessions
- Primary accessionQ38856
- Secondary accessions
Proteomes
Organism-specific databases
Genome annotation databases
Subcellular Location
UniProt Annotation
GO Annotation
Cell membrane ; Multi-pass membrane protein
Note: Cycles constitutively between early endosomes and the plasma membrane and is targeted to the vacuole for degradation.
Features
Showing features for topological domain, transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Topological domain | 23-52 | Extracellular | ||||
Sequence: ISPATSTAPEECGSESANPCVNKAKALPLK | ||||||
Transmembrane | 53-73 | Helical | ||||
Sequence: VIAIFVILIASMIGVGAPLFS | ||||||
Topological domain | 74-84 | Cytoplasmic | ||||
Sequence: RNVSFLQPDGN | ||||||
Transmembrane | 85-105 | Helical | ||||
Sequence: IFTIIKCFASGIILGTGFMHV | ||||||
Topological domain | 106-125 | Extracellular | ||||
Sequence: LPDSFEMLSSICLEENPWHK | ||||||
Transmembrane | 126-146 | Helical | ||||
Sequence: FPFSGFLAMLSGLITLAIDSM | ||||||
Topological domain | 147-192 | Cytoplasmic | ||||
Sequence: ATSLYTSKNAVGIMPHGHGHGHGPANDVTLPIKEDDSSNAQLLRYR | ||||||
Transmembrane | 193-213 | Helical | ||||
Sequence: VIAMVLELGIIVHSVVIGLSL | ||||||
Topological domain | 214-224 | Extracellular | ||||
Sequence: GATSDTCTIKG | ||||||
Transmembrane | 225-245 | Helical | ||||
Sequence: LIAALCFHQMFEGMGLGGCIL | ||||||
Topological domain | 246-254 | Cytoplasmic | ||||
Sequence: QAEYTNMKK | ||||||
Transmembrane | 255-275 | Helical | ||||
Sequence: FVMAFFFAVTTPFGIALGIAL | ||||||
Topological domain | 276-286 | Extracellular | ||||
Sequence: STVYQDNSPKA | ||||||
Transmembrane | 287-307 | Helical | ||||
Sequence: LITVGLLNACSAGLLIYMALV | ||||||
Topological domain | 308-326 | Cytoplasmic | ||||
Sequence: DLLAAEFMGPKLQGSIKMQ | ||||||
Transmembrane | 327-347 | Helical | ||||
Sequence: FKCLIAALLGCGGMSIIAKWA |
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Plants exhibit a lethal chlorotic phenotype.
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 104 | Suppresses transport. | ||||
Sequence: H → A | ||||||
Mutagenesis | 108 | Abolishes iron and manganese transport. | ||||
Sequence: D → A | ||||||
Mutagenesis | 111 | Abolishes zinc transport. Abolishes iron and manganese transport; when associated with A-108. | ||||
Sequence: E → A | ||||||
Mutagenesis | 144 | Abolishes iron and manganese transport. Reduces cadmium transport. | ||||
Sequence: D → A | ||||||
Mutagenesis | 154 | Suppresses ubiquitination and loss of activity; when associated with R-179. | ||||
Sequence: K → R | ||||||
Mutagenesis | 179 | Suppresses ubiquitination and loss of activity; when associated with R-154. | ||||
Sequence: K → R | ||||||
Mutagenesis | 205 | Suppresses transport. | ||||
Sequence: H → A or E | ||||||
Mutagenesis | 206 | Suppresses transport. | ||||
Sequence: S → A | ||||||
Mutagenesis | 232 | Suppresses transport. | ||||
Sequence: H → A | ||||||
Mutagenesis | 236 | Suppresses transport. | ||||
Sequence: E → A |
Variants
We now provide the "Disease & Variants" viewer in its own tab.
The viewer provides 12 variants from UniProt as well as other sources including ClinVar and dbSNP.
PTM/Processing
Features
Showing features for signal, chain, cross-link.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Signal | 1-22 | |||||
Sequence: MASNSALLMKTIFLVLIFVSFA | ||||||
Chain | PRO_0000041636 | 23-347 | Fe2+ transport protein 1 | |||
Sequence: ISPATSTAPEECGSESANPCVNKAKALPLKVIAIFVILIASMIGVGAPLFSRNVSFLQPDGNIFTIIKCFASGIILGTGFMHVLPDSFEMLSSICLEENPWHKFPFSGFLAMLSGLITLAIDSMATSLYTSKNAVGIMPHGHGHGHGPANDVTLPIKEDDSSNAQLLRYRVIAMVLELGIIVHSVVIGLSLGATSDTCTIKGLIAALCFHQMFEGMGLGGCILQAEYTNMKKFVMAFFFAVTTPFGIALGIALSTVYQDNSPKALITVGLLNACSAGLLIYMALVDLLAAEFMGPKLQGSIKMQFKCLIAALLGCGGMSIIAKWA | ||||||
Cross-link | 154 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) | ||||
Sequence: K | ||||||
Cross-link | 179 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) | ||||
Sequence: K |
Post-translational modification
Monoubiquitinated on several Lys residues. Monoubiquitination controls trafficking from the plasma membrane and targeting to the vacuole.
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Tissue specificity
Expressed in the external cell layers of the root including the lateral branching zone. Also detected in flowers before pollination.
Induction
In roots by iron starvation.
Gene expression databases
Interaction
Subunit
Interacts with FREE1.
Binary interactions
Type | Entry 1 | Entry 2 | Number of experiments | Intact | |
---|---|---|---|---|---|
BINARY | Q38856 | UBQ16 P59263 | 3 | EBI-15928951, EBI-1541543 |
Protein-protein interaction databases
Structure
Sequence & Isoform
- Sequence statusComplete
- Sequence processingThe displayed sequence is further processed into a mature form.
This entry describes 2 isoforms produced by Alternative splicing.
Q38856-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length347
- Mass (Da)36,727
- Last updated2011-05-31 v2
- Checksum1F94091668994EA4
Q38856-2
- Name2
- NoteMay be due to an intron retention.
Sequence caution
Features
Showing features for alternative sequence.
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AL024486 EMBL· GenBank· DDBJ | CAA19686.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation | |
AL161551 EMBL· GenBank· DDBJ | CAB78971.1 EMBL· GenBank· DDBJ | Genomic DNA | Different initiation | |
CP002687 EMBL· GenBank· DDBJ | AEE84216.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP002687 EMBL· GenBank· DDBJ | AEE84217.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
AY087095 EMBL· GenBank· DDBJ | AAM64655.1 EMBL· GenBank· DDBJ | mRNA | ||
U27590 EMBL· GenBank· DDBJ | AAB01678.1 EMBL· GenBank· DDBJ | mRNA | Different initiation |