Q38856 · IRT1_ARATH

Function

function

High-affinity iron transporter that plays a key role in the uptake of iron from the rhizosphere across the plasma membrane in the root epidermal layer. Acts as the principal regulator of iron homeostasis in planta. Also mediates the heavy metals uptake under iron-deficiency by its ability to transport cobalt, cadmium, manganese and/or zinc ions.

Miscellaneous

Inhibited by cadmium and Fe2+ ions and at 100-fold excess inhibited by cobalt, manganese and zinc ions.
The availability of secondary non-iron metal substrates (Zn, Mn, and Co) controls the localization of IRT1 between the outer polar domain of the plasma membrane and early endosome/trans-Golgi network in root epidermal cells.

GO annotations

all annotationsall molecular functionnucleotide bindingmolecular_functionnucleic acid bindingdna bindingchromatin bindingdna-binding transcription factor activityrna bindingcytoskeletal motor activitycatalytic activitynuclease activitysignaling receptor bindingstructural molecule activitytransporter activitybindingprotein bindingtranslation factor activity, rna bindinglipid bindingkinase activitytransferase activityhydrolase activityoxygen bindingenzyme regulator activitycarbohydrate bindingsignaling receptor activitytranslation regulator activitytranscription regulator activityother molecular functionall biological processcarbohydrate metabolic processgeneration of precursor metabolites and energynucleobase-containing compound metabolic processdna metabolic processtranslationlipid metabolic processtransportresponse to stresscell cyclecell communicationsignal transductioncell-cell signalingmulticellular organism developmentcircadian rhythmbiological_processmetabolic processcatabolic processbiosynthetic processresponse to light stimulusresponse to external stimulustropismresponse to biotic stimulusresponse to abiotic stimulusresponse to endogenous stimulusembryo developmentpost-embryonic developmentfruit ripeningabscissionpollinationflower developmentcellular processprogrammed cell deathphotosynthesiscellular component organizationcell growthprotein metabolic processcellular homeostasissecondary metabolic processreproductive processcell differentiationprotein modification processgrowthepigenetic regulation of gene expressionresponse to chemicalanatomical structure developmentregulation of molecular functionother biological processall cellular componentcellular_componentextracellular regioncell wallintracellular anatomical structurenucleusnuclear envelopenucleoplasmnucleoluscytoplasmmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuscytosolribosomecytoskeletonplasma membranechloroplastplastidthylakoidmembraneexternal encapsulating structureother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentearly endosome
Cellular Componentendosome
Cellular Componentplasma membrane
Cellular Componenttrans-Golgi network
Cellular Componentvacuole
Molecular Functioncopper ion transmembrane transporter activity
Molecular Functioniron ion transmembrane transporter activity
Molecular Functionzinc ion transmembrane transporter activity
Biological Processmetal ion transport
Biological Processnickel cation transport
Biological Processresponse to bacterium

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Fe(2+) transport protein 1
  • Alternative names
    • Fe(II) transport protein 1
    • Iron-regulated transporter 1

Gene names

    • Name
      IRT1
    • ORF names
      T16H5.50
    • Ordered locus names
      At4g19690

Organism names

  • Taxonomic identifier
  • Strains
    • cv. Columbia
    • cv. Landsberg erecta
  • Taxonomic lineage
    Eukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > rosids > malvids > Brassicales > Brassicaceae > Camelineae > Arabidopsis

Accessions

  • Primary accession
    Q38856
  • Secondary accessions
    • Q8LBP0

Proteomes

Organism-specific databases

Genome annotation databases

Subcellular Location

Cell membrane
; Multi-pass membrane protein
Early endosome
Vacuole
Note: Cycles constitutively between early endosomes and the plasma membrane and is targeted to the vacuole for degradation.

Features

Showing features for topological domain, transmembrane.

TypeIDPosition(s)Description
Topological domain23-52Extracellular
Transmembrane53-73Helical
Topological domain74-84Cytoplasmic
Transmembrane85-105Helical
Topological domain106-125Extracellular
Transmembrane126-146Helical
Topological domain147-192Cytoplasmic
Transmembrane193-213Helical
Topological domain214-224Extracellular
Transmembrane225-245Helical
Topological domain246-254Cytoplasmic
Transmembrane255-275Helical
Topological domain276-286Extracellular
Transmembrane287-307Helical
Topological domain308-326Cytoplasmic
Transmembrane327-347Helical

Keywords

Phenotypes & Variants

Disruption phenotype

Plants exhibit a lethal chlorotic phenotype.

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis104Suppresses transport.
Mutagenesis108Abolishes iron and manganese transport.
Mutagenesis111Abolishes zinc transport. Abolishes iron and manganese transport; when associated with A-108.
Mutagenesis144Abolishes iron and manganese transport. Reduces cadmium transport.
Mutagenesis154Suppresses ubiquitination and loss of activity; when associated with R-179.
Mutagenesis179Suppresses ubiquitination and loss of activity; when associated with R-154.
Mutagenesis205Suppresses transport.
Mutagenesis206Suppresses transport.
Mutagenesis232Suppresses transport.
Mutagenesis236Suppresses transport.

Variants

We now provide the "Disease & Variants" viewer in its own tab.

The viewer provides 12 variants from UniProt as well as other sources including ClinVar and dbSNP.

Go to variant viewer

PTM/Processing

Features

Showing features for signal, chain, cross-link.

TypeIDPosition(s)Description
Signal1-22
ChainPRO_000004163623-347Fe2+ transport protein 1
Cross-link154Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)
Cross-link179Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)

Post-translational modification

Monoubiquitinated on several Lys residues. Monoubiquitination controls trafficking from the plasma membrane and targeting to the vacuole.

Keywords

Proteomic databases

PTM databases

Expression

Tissue specificity

Expressed in the external cell layers of the root including the lateral branching zone. Also detected in flowers before pollination.

Induction

In roots by iron starvation.

Gene expression databases

Interaction

Subunit

Interacts with FREE1.

Binary interactions

TypeEntry 1Entry 2Number of experimentsIntact
BINARY Q38856UBQ16 P592633EBI-15928951, EBI-1541543

Protein-protein interaction databases

Family & Domains

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete
  • Sequence processing
    The displayed sequence is further processed into a mature form.

This entry describes 2 isoforms produced by Alternative splicing.

Q38856-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    347
  • Mass (Da)
    36,727
  • Last updated
    2011-05-31 v2
  • Checksum
    1F94091668994EA4
MASNSALLMKTIFLVLIFVSFAISPATSTAPEECGSESANPCVNKAKALPLKVIAIFVILIASMIGVGAPLFSRNVSFLQPDGNIFTIIKCFASGIILGTGFMHVLPDSFEMLSSICLEENPWHKFPFSGFLAMLSGLITLAIDSMATSLYTSKNAVGIMPHGHGHGHGPANDVTLPIKEDDSSNAQLLRYRVIAMVLELGIIVHSVVIGLSLGATSDTCTIKGLIAALCFHQMFEGMGLGGCILQAEYTNMKKFVMAFFFAVTTPFGIALGIALSTVYQDNSPKALITVGLLNACSAGLLIYMALVDLLAAEFMGPKLQGSIKMQFKCLIAALLGCGGMSIIAKWA

Q38856-2

  • Name
    2
  • Note
    May be due to an intron retention.
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Sequence caution

The sequence AAB01678.1 differs from that shown. Reason: Erroneous initiation Truncated N-terminus.
The sequence CAA19686.1 differs from that shown. Reason: Erroneous initiation Truncated N-terminus.
The sequence CAB78971.1 differs from that shown. Reason: Erroneous initiation Truncated N-terminus.

Features

Showing features for alternative sequence.

TypeIDPosition(s)Description
Alternative sequenceVSP_008361198-211in isoform 2
Alternative sequenceVSP_008362212-347in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AL024486
EMBL· GenBank· DDBJ
CAA19686.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation
AL161551
EMBL· GenBank· DDBJ
CAB78971.1
EMBL· GenBank· DDBJ
Genomic DNA Different initiation
CP002687
EMBL· GenBank· DDBJ
AEE84216.1
EMBL· GenBank· DDBJ
Genomic DNA
CP002687
EMBL· GenBank· DDBJ
AEE84217.1
EMBL· GenBank· DDBJ
Genomic DNA
AY087095
EMBL· GenBank· DDBJ
AAM64655.1
EMBL· GenBank· DDBJ
mRNA
U27590
EMBL· GenBank· DDBJ
AAB01678.1
EMBL· GenBank· DDBJ
mRNA Different initiation

Genome annotation databases

Similar Proteins

Disclaimer

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