Q32RX0 · TILS_STAPU
- ProteintRNA(Ile)-lysidine synthase, chloroplastic
- GenetilS
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids493 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.
Catalytic activity
- cytidine34 in tRNA(Ile2) + L-lysine + ATP = lysidine34 in tRNA(Ile2) + AMP + diphosphate + H+
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | chloroplast | |
Molecular Function | ATP binding | |
Molecular Function | tRNA(Ile)-lysidine synthase activity | |
Biological Process | tRNA modification |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA(Ile)-lysidine synthase, chloroplastic
- EC number
- Alternative names
Gene names
Encoded on
- Chloroplast
Organism names
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Zygnemophyceae > Zygnematophycidae > Desmidiales > Desmidiaceae > Staurastrum
Accessions
- Primary accessionQ32RX0
Subcellular Location
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000277071 | 1-493 | tRNA(Ile)-lysidine synthase, chloroplastic | |||
Sequence: MQNNKDNYLKFTKMDKKSIRAPYDQNDLSKKIQRIKKREDIFLLYKINNIITEHTLLQPKQRILIAVSGGQDSICLLRILFDLQRKWSWKLGIIHCDHRWNSRSKIQAEHVACLAINLQINYHEGIAIESVQKESIARLWRYNVIQSVAISNNYTAIITGHNASDRIETLLYNLMRGSGLHGLQSIKWKRNLCFSHFTRSVLSKIKFSFLVKKLKFIKTFMDHEDFQGNHFFFLKKRKQLHLIRPFLETTRTEIRNILTIWNFPSWPDVSNKELRIRRNRIRHRVIPYIRIHYNPNIDQTLVRWAEIVQSETFYLEQLTNYILFKIEIKKKISSISLKIPLEQLRKQKEPSEEKLNFYQSAIPVDLLRSLPVAIQRRVLKQYIYINTNRILGFQYIEQIRLSCLFSLQDSLRKFLHRHRCRPIYLSEHISTHSMKSSTSDTCIDWKGPKEKKKLVTPWLIFPGGIKFLIRKNYLFIFSPKNSSLTIKRPRIEQ |
Structure
Sequence
- Sequence statusComplete
- Length493
- Mass (Da)58,834
- Last updated2005-12-06 v1
- Checksum541FCC83DC91FA7C
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AY958085 EMBL· GenBank· DDBJ | AAX45766.1 EMBL· GenBank· DDBJ | Genomic DNA |