Q32RX0 · TILS_STAPU

Function

function

Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine.

Catalytic activity

Features

Showing features for binding site.

149350100150200250300350400450
TypeIDPosition(s)Description
Binding site68-73ATP (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentchloroplast
Molecular FunctionATP binding
Molecular FunctiontRNA(Ile)-lysidine synthase activity
Biological ProcesstRNA modification

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    tRNA(Ile)-lysidine synthase, chloroplastic
  • EC number
  • Alternative names
    • tRNA(Ile)-2-lysyl-cytidine synthase
    • tRNA(Ile)-lysidine synthetase

Gene names

    • Name
      tilS

Encoded on

  • Chloroplast

Organism names

Accessions

  • Primary accession
    Q32RX0

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002770711-493tRNA(Ile)-lysidine synthase, chloroplastic

Structure

Family & Domains

Domain

The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.

Sequence similarities

Belongs to the tRNA(Ile)-lysidine synthase family.

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    493
  • Mass (Da)
    58,834
  • Last updated
    2005-12-06 v1
  • Checksum
    541FCC83DC91FA7C
MQNNKDNYLKFTKMDKKSIRAPYDQNDLSKKIQRIKKREDIFLLYKINNIITEHTLLQPKQRILIAVSGGQDSICLLRILFDLQRKWSWKLGIIHCDHRWNSRSKIQAEHVACLAINLQINYHEGIAIESVQKESIARLWRYNVIQSVAISNNYTAIITGHNASDRIETLLYNLMRGSGLHGLQSIKWKRNLCFSHFTRSVLSKIKFSFLVKKLKFIKTFMDHEDFQGNHFFFLKKRKQLHLIRPFLETTRTEIRNILTIWNFPSWPDVSNKELRIRRNRIRHRVIPYIRIHYNPNIDQTLVRWAEIVQSETFYLEQLTNYILFKIEIKKKISSISLKIPLEQLRKQKEPSEEKLNFYQSAIPVDLLRSLPVAIQRRVLKQYIYINTNRILGFQYIEQIRLSCLFSLQDSLRKFLHRHRCRPIYLSEHISTHSMKSSTSDTCIDWKGPKEKKKLVTPWLIFPGGIKFLIRKNYLFIFSPKNSSLTIKRPRIEQ

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AY958085
EMBL· GenBank· DDBJ
AAX45766.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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