Q32NN2 · QKIA_XENLA

Function

function

RNA reader protein, which recognizes and binds specific RNAs, thereby regulating RNA metabolic processes, such as pre-mRNA splicing, circular RNA (circRNA) formation, mRNA export, mRNA stability and/or translation (By similarity).
Involved in various cellular processes, such as mRNA storage into stress granules, apoptosis, interferon response, glial cell fate and development. Binds to the 5'-NACUAAY-N(1,20)-UAAY-3' RNA core sequence (By similarity).
Acts as a mRNA modification reader that specifically recognizes and binds mRNA transcripts modified by internal N7-methylguanine (m7G) (By similarity).
Promotes the formation of circular RNAs (circRNAs): acts by binding to sites flanking circRNA-forming exons. CircRNAs are produced by back-splicing circularization of pre-mRNAs. Required to protect and promote stability of mRNAs which promotes oligodendrocyte differentiation (By similarity).
Acts as an important regulator of muscle development (By similarity).
Essential for notochord development (PubMed:9303534).

GO annotations

all annotationsall molecular functionvirus receptor activitydna bindingrna bindingcytoskeletal motor activitycatalytic activitygtpase activitystructural molecule activitytransporter activitycytoskeletal protein bindinglipid bindingcyclase activityantioxidant activityoxidoreductase activitytransferase activityhydrolase activitylyase activityisomerase activityligase activityprotein tag activitycargo receptor activityhistone bindingprotein folding chaperonetranslation regulator activitynutrient reservoir activityreceptor ligand activitymolecular transducer activitymolecular adaptor activitytoxin activitycell adhesion mediator activitymolecular function regulator activityvirus coreceptor activitycatalytic activity, acting on a proteincatalytic activity, acting on dnacatalytic activity, acting on rnamolecular carrier activitytranscription regulator activitygeneral transcription initiation factor activitymolecular sensor activitymolecular sequestering activityatp-dependent activityother molecular functionall biological processmitotic cell cyclecytokinesiscytoplasmic translationimmune system processmuscle system processcirculatory system processrenal system processrespiratory system processcarbohydrate metabolic processgeneration of precursor metabolites and energydna replicationdna repairdna recombinationchromatin organizationdna-templated transcriptionregulation of dna-templated transcriptiontrna metabolic processprotein foldingprotein glycosylationamino acid metabolic processmodified amino acid metabolic processlipid metabolic processvitamin metabolic processsulfur compound metabolic processintracellular protein transportnucleocytoplasmic transportautophagyinflammatory responsemitochondrion organizationcytoskeleton organizationmicrotubule-based movementperoxisome organizationlysosome organizationchromosome segregationcell adhesionestablishment or maintenance of cell polarityprogrammed cell deathphotosynthesismrna metabolic processsnrna metabolic processvesicle-mediated transportreproductive processdigestive system processsignalingcell differentiationprotein catabolic processextracellular matrix organizationregulatory ncrna-mediated gene silencingtelomere organizationcell junction organizationwound healingribosome biogenesiscilium organizationanatomical structure developmentcell motilitynervous system processendocrine processprotein maturationtransmembrane transportnucleobase-containing small molecule metabolic processhepaticobiliary system processmembrane organizationprotein-containing complex assemblycell wall organization or biogenesisnitrogen cycle metabolic processprotein localization to plasma membranedefense response to other organismdetoxificationmeiotic nuclear divisionmitotic nuclear divisionmitochondrial gene expressioncarbohydrate derivative metabolic processother biological processall cellular componentnuclear chromosomeextracellular regionextracellular spacecell wallnucleusnuclear envelopenucleoplasmchromosomenucleolusmitochondrionlysosomeendosomevacuoleperoxisomeendoplasmic reticulumgolgi apparatuslipid dropletmicrotubule organizing centercytosolribosomecytoskeletonplasma membraneciliumplastidthylakoidexternal encapsulating structureextracellular matrixcytoplasmic vesicleorganelleother cellular component
Cell color indicative of number of GO terms
AspectTerm
Cellular Componentcytosol
Cellular Componentnucleus
Molecular Functionidentical protein binding
Molecular Functionpoly(U) RNA binding
Molecular Functionsingle-stranded RNA binding
Biological ProcessmRNA transport
Biological Processnegative regulation of macrophage differentiation
Biological Processnotochord cell differentiation
Biological Processnotochord development
Biological Processnotochord formation
Biological Processregulation of astrocyte differentiation
Biological Processregulation of translation
Biological Processspliceosome-depend formation of circular RNA

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    KH domain-containing RNA-binding protein qki.S
  • Alternative names
    • Protein quaking-A
      (Xqua)

Gene names

    • Name
      qki.S

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Amphibia > Batrachia > Anura > Pipoidea > Pipidae > Xenopodinae > Xenopus > Xenopus

Accessions

  • Primary accession
    Q32NN2

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00002393801-341KH domain-containing RNA-binding protein qki.S

Expression

Developmental stage

First detected in the chordamesoderm of the dorsal blastopore lip of the mid-gastrula embryo (stage 11). This tissue, which differentiates into the notochord. In late gastrula embryos (stage 13), it is highly expressed in the notochord, and the original expression domain expands to include the tissue surrounding the blastopore. In neurula embryos, it is maintained in the notochord and the circumblastoporal region and now also extends to the paraxial mesoderm and the neuroectoderm. By the tailbud stage, it is expressed in various mesodermal and neural tissues. Highly expressed in the brain and the neural tube. Maintained throughout development to the tadpole stage (stage 35).

Gene expression databases

    • 734923Expressed in blastula and 19 other cell types or tissues

Interaction

Subunit

Homodimer; does not require RNA to homodimerize.

Family & Domains

Features

Showing features for domain, motif.

TypeIDPosition(s)Description
Domain88-154KH
Motif324-330Nuclear localization signal

Sequence similarities

Belongs to the quaking family.

Phylogenomic databases

Family and domain databases

Sequence & Isoforms

Align isoforms (4)
  • Sequence status
    Complete

This entry describes 4 isoforms produced by Alternative splicing.

Q32NN2-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    341
  • Mass (Da)
    37,879
  • Last updated
    2006-06-13 v2
  • Checksum
    F39F36CB80DE77A1
MVGEMETKEKPKPTPDYLMQLMNDKKLMSSLPNFCGIFTHLERLLDEEISRVRKDMYNDTMNSSSNEKRTSELPDGIGPIVQLQEKLYVPVKEYPDFNFVGRILGPRGLTAKQLEAETGCKIMVRGKGSMRDKKKEEQNRGKPNWEHLNEDLHVLITVEDAQNRAELKLKRAVEEVKKLLVPAAEGEDSLKKMQLMELAILNGTYRDANLKSPALAFSLAATGQAPRIITGPAPVLSPAALRTPTPAGHTLMPLIRQIQTAVMPNGTPHPTATLMQQAPEGGLIYTPYEYPYTLAPATSILEYPIEASGVLGAVATKVRRHDMRVHPYQRIVTADRAATGN

Q32NN2-2

  • Name
    2
  • Synonyms
    Xqua365
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 1-1: M → MFFGHAGCDIRNSAAPAFGLFDWNM

Q32NN2-3

  • Name
    3
  • Synonyms
    Xqua357
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 1-1: M → MFFGHAGCDIRNSAAPAFGLFDWNM
    • 214-221: Missing

Q32NN2-4

  • Name
    4
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical

Computationally mapped potential isoform sequences

There are 7 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A8J1KRR9A0A8J1KRR9_XENLAqki.S379
A0A8J1KRN5A0A8J1KRN5_XENLAqki.S342
A0A1L8G2F2A0A1L8G2F2_XENLAqki.S365
A0A8J0VEW5A0A8J0VEW5_XENLAqki.S380
A0A8J0VEX0A0A8J0VEX0_XENLAqki.S358
A0A8J0VE05A0A8J0VE05_XENLAqki.S372
A0A8J0VJC2A0A8J0VJC2_XENLAqki.S343

Features

Showing features for alternative sequence, sequence conflict.

TypeIDPosition(s)Description
Alternative sequenceVSP_0192061in isoform 2 and isoform 3
Sequence conflict194in Ref. 1 and 4
Alternative sequenceVSP_019207214-221in isoform 3 and isoform 4

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BC108554
EMBL· GenBank· DDBJ
AAI08555.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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