Q31JE7 · MNMC_HYDCU

Function

function

Catalyzes the last two steps in the biosynthesis of 5-methylaminomethyl-2-thiouridine (mnm5s2U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm5s2U34 to nm5s2U34, followed by the transfer of a methyl group from S-adenosyl-L-methionine to nm5s2U34, to form mnm5s2U34.

Catalytic activity

Cofactor

FAD (UniProtKB | Rhea| CHEBI:57692 )

GO annotations

AspectTerm
Cellular Componentcytoplasm
Molecular Functionflavin adenine dinucleotide binding
Molecular Functionoxidoreductase activity, acting on the CH-NH group of donors
Molecular FunctiontRNA (5-methylaminomethyl-2-thiouridylate)(34)-methyltransferase activity
Biological Processmethylation
Biological ProcesstRNA wobble base modification

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC
  • Short names
    tRNA mnm(5)s(2)U biosynthesis bifunctional protein

Including 2 domains:

  • Recommended name
    tRNA (mnm(5)s(2)U34)-methyltransferase
  • EC number
  • Recommended name
    FAD-dependent cmnm(5)s(2)U34 oxidoreductase
  • EC number

Gene names

    • Name
      mnmC
    • Ordered locus names
      Tcr_0130

Organism names

Accessions

  • Primary accession
    Q31JE7

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00003480441-674tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region1-248tRNA (mnm5s2U34)-methyltransferase
Region276-674FAD-dependent cmnm5s2U34 oxidoreductase

Sequence similarities

In the N-terminal section; belongs to the methyltransferase superfamily. tRNA (mnm(5)s(2)U34)-methyltransferase family.
In the C-terminal section; belongs to the DAO family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    674
  • Mass (Da)
    74,898
  • Last updated
    2005-12-06 v1
  • Checksum
    6E1F495A757B7FC1
MAASSLPSHNSKFEPKLERAQIAWSEEGVPTSTMFDDVYYSKLDGLAEVNYIFLKHNQLPERFETLLPNSRFCILETGFGAGLNFLTTCLLWLEKSPADAQLHFISLEKFPLTRSELAKAHHAFEEVSEPSNALLDVYPLHLPGWHDVFLYDQRIRLTLWFGHVMKGLPEFDVQVDAWFLDGFTPRKNPDMWQSELYLQMARLSNEATTFATFTAAGDVRRGLAQYGFEVEKAPGYAQKREMCFGRYAHSRPFSSKAPWFERAKGGHPSPKKAIVIGAGLAGATVAYQLASQGLSVTVLEQEKAVAQQASGNLAGAIHPLVTADWNVRSQFYLKGFECSLRWLRPWFDSHSIVGQQNGLMQLAMTETMLQRLQEALTRVGLPKSFAYWCDADQASDLIGQKTDHEGLFFPEAGWVQPTSVVTQCLAHEAIALKQEEAVVSIQPVPDQNSQDWQIMTPNSTYQADVVVVATGALNGDLNEQLNLPIRPVKGQVSHFKAADLKGALKTTVTHRGYSVCGDFGAQSPYAAISGATFEAPDLSPILSDASHIENQRLATEALPNWLADSTDMAGKVGFRPTTPDHLPLIGAVPDFEWMKTAYFEQSHTHAVYRYAEQRYQPGLYVSNGHGARGLMSVFLAAEMIGAMVTGDAQVMPKSLYHAVHPARFAIRHWRSGKR

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000109
EMBL· GenBank· DDBJ
ABB40726.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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