Q31HD6 · CSOCA_HYDCU
- ProteinCarboxysome shell carbonic anhydrase
- GenecsoS3
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids528 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Reversible hydration of carbon dioxide. This bacteria encodes at least 3 CA enzymes (PubMed:20400567).
Essential for chemolithotrophic carbon dioxide fixation, supplies CO2 to RuBisCO (ribulose bisphosphate carboxylase, cbbL-cbbS) in the carboxysome (Probable).
Essential for chemolithotrophic carbon dioxide fixation, supplies CO2 to RuBisCO (ribulose bisphosphate carboxylase, cbbL-cbbS) in the carboxysome (Probable).
Catalytic activity
- H+ + hydrogencarbonate = CO2 + H2O
Cofactor
Note: Binds 1 Zn2+ per monomer.
Activity regulation
Inhibited by ethoxyzolamide and dithiothreitol (in crude extracts upon expression in E.coli).
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | carboxysome | |
Molecular Function | carbonate dehydratase activity | |
Molecular Function | metal ion binding | |
Biological Process | carbon fixation |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameCarboxysome shell carbonic anhydrase
- EC number
- Short namesCsoSCA
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Gammaproteobacteria > Thiotrichales > Piscirickettsiaceae > Hydrogenovibrio
Accessions
- Primary accessionQ31HD6
Subcellular Location
UniProt Annotation
GO Annotation
Note: This bacterium makes alpha-type carboxysomes.
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Cells do not grow in low DIC levels. Cells lose carboxysomes when grown under low DIC conditions. Accumulates intracellular DIC normally, poor CO2 fixation.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000452063 | 1-528 | Carboxysome shell carbonic anhydrase | |||
Sequence: MNRLKKSHRQKSLFWRPIAPNPRWQKENPTAHGSTDTGGFGYNGGNEEVKTSSTMMNGIHALVNERQNEWLRGYEVDIKSRFDNIESVLKDILAQQSQLNFVSWANQQLFAKLGVSLTEQDWQSGVQLQSQKGFQFLYGKTLFAQFMRMSEDFFVNDPLSGQRKQEAERMFKEAGFHAVGIAPCADGRLAHILSYVLRLPYALARRKAHAGVMFDVSESVRNWVFIEHTRFRDGQPNLADEPTRYLKIAVYHFSKADPTHQGCAAHGSDDHKAAQAALQKLKDFKQAIENRFGCGSTVQTLLLGLNTDDDSMKVHIPNASGEVCLDRYVETEQLYQATMNLPDSEAKQALENAIVTCNQALGSTAPQPELVKLLSWLIGNNFSQIAYVNQYENGCYSDIGHAERFIGIGNGFEEVQLRNLSYYSFLDTVEEGVNDVDVGIKIFKGLNVKKGLPIPIIIRCDYDGRVPGSKDRAEAKALRIEKALHNRYQELSAPGLLQTLPTLRDFTSCKPAERLPGLADLSAKQRTA |
Expression
Induction
Induced by growth in low levels of dissolved inorganic carbon (DIC) (at protein level).
Interaction
Structure
Family & Domains
Features
Showing features for region, compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 17-47 | Disordered | ||||
Sequence: PIAPNPRWQKENPTAHGSTDTGGFGYNGGNE | ||||||
Compositional bias | 27-47 | Polar residues | ||||
Sequence: ENPTAHGSTDTGGFGYNGGNE |
Sequence similarities
Belongs to the beta-class carbonic anhydrase family. CsoSCA subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length528
- Mass (Da)59,254
- Last updated2005-12-06 v1
- ChecksumBFB6FFBD712B8484
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 27-47 | Polar residues | ||||
Sequence: ENPTAHGSTDTGGFGYNGGNE |
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000109 EMBL· GenBank· DDBJ | ABB41437.1 EMBL· GenBank· DDBJ | Genomic DNA |