Q312S3 · ISLB_OLEA2

Function

function

Involved in an anaerobic respiration pathway that converts the sulfonate isethionate (2-hydroxyethanesulfonate) to ammonia, acetate and sulfide. Catalyzes activation of the isethionate sulfite-lyase IslA under anaerobic conditions by generation of an organic free radical on a glycine residue, via a homolytic cleavage of S-adenosyl-L-methionine (SAM).

Catalytic activity

Cofactor

[4Fe-4S] cluster (UniProtKB | Rhea| CHEBI:49883 )

Note: Binds 3 [4Fe-4S] clusters. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.

Pathway

Organosulfur degradation; alkanesulfonate degradation.

Features

Showing features for binding site.

TypeIDPosition(s)Description
Binding site36[4Fe-4S] cluster 1 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site40[4Fe-4S] cluster 1 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site42-44S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site43[4Fe-4S] cluster 1 (UniProtKB | ChEBI); 4Fe-4S-S-AdoMet
Binding site62[4Fe-4S] cluster 2 (UniProtKB | ChEBI)
Binding site68[4Fe-4S] cluster 2 (UniProtKB | ChEBI)
Binding site71[4Fe-4S] cluster 2 (UniProtKB | ChEBI)
Binding site75[4Fe-4S] cluster 3 (UniProtKB | ChEBI)
Binding site95[4Fe-4S] cluster 3 (UniProtKB | ChEBI)
Binding site98[4Fe-4S] cluster 3 (UniProtKB | ChEBI)
Binding site102[4Fe-4S] cluster 3 (UniProtKB | ChEBI)
Binding site106[4Fe-4S] cluster 2 (UniProtKB | ChEBI)
Binding site146S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site195-197S-adenosyl-L-methionine (UniProtKB | ChEBI)
Binding site268S-adenosyl-L-methionine (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Function4 iron, 4 sulfur cluster binding
Molecular Functionmetal ion binding
Molecular Functionoxidoreductase activity
Biological Processalkanesulfonate catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Isethionate sulfite-lyase activating enzyme
  • EC number
  • Alternative names
    • GRE activase IslB
    • Glycyl-radical enzyme activating enzyme IslB

Gene names

    • Name
      islB
    • Ordered locus names
      Dde_1272

Organism names

Accessions

  • Primary accession
    Q312S3

Proteomes

Phenotypes & Variants

Disruption phenotype

Cells lacking this gene lose the ability to grow with isethionate as the terminal electron acceptor.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004509461-309Isethionate sulfite-lyase activating enzyme

Expression

Induction

Highly up-regulated in the presence of isethionate.

Interaction

Subunit

Monomer.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain22-309Radical SAM core
Domain53-854Fe-4S ferredoxin-type 1
Domain86-1174Fe-4S ferredoxin-type 2

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    309
  • Mass (Da)
    34,567
  • Last updated
    2005-12-06 v1
  • Checksum
    8FE38F4981B65817
MSSFEDKKTTGITFNIQKYSVHDGPGIRTVVFLKGCPLRCRWCSNPESQRRRIELAYNTGRCLTLTKCVRCVEVCTMNAITRADDDTISIDRALCEECGMFCAEACPSKALITYGTTRTVDEVLNVVEQDSVFYARSGGGITLSGGEPFAQPAFALALLREARRRHIHTAVETCGYASWSDMEPALEYVKFVHYDIKSLDDEKHRSATGVSNVRIIENLRNIRSRFPALKVVVRTPVIPGFNDTEEDIRAIARLTAELEVEYQLLPYHRLGTQKYTFLDRQAPMGEVVLDEQVMTALNAVVAAEHATDG

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000112
EMBL· GenBank· DDBJ
ABB38073.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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