Q2YPC4 · TCPB_BRUA2
- ProteinProbable 2' cyclic ADP-D-ribose synthase TcpB
- Genebtp1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids250 (go to sequence)
- Protein existenceInferred from homology
- Annotation score4/5
Function
function
Virulence factor that interferes with host Toll-like receptor 2 (TLR2) signaling, resulting in the reduction of dendritic cell maturation, inhibition of pro-inflammatory cytokine secretion and impaired NF-kappa-B activation in macrophages (PubMed:18266466).
Also acts on host TLR4. Binds host lipids. Has NAD+ hydrolase (NADase) activity, catalyzes cleavage of NAD+ into ADP-D-ribose (ADPR) and nicotinamide, also generates a cyclization variant of cyclic ADPR (cADPR), termed v-cADPR (probably 2'cADPR) (By similarity).
Also acts on host TLR4. Binds host lipids. Has NAD+ hydrolase (NADase) activity, catalyzes cleavage of NAD+ into ADP-D-ribose (ADPR) and nicotinamide, also generates a cyclization variant of cyclic ADPR (cADPR), termed v-cADPR (probably 2'cADPR) (By similarity).
Catalytic activity
- H2O + NAD+ = ADP-D-ribose + H+ + nicotinamideThis reaction proceeds in the forward direction.
- NAD+ = 2'cADPR + H+ + nicotinamide
Features
Showing features for active site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Active site | 192 | |||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | extracellular region | |
Cellular Component | host cell plasma membrane | |
Cellular Component | membrane | |
Molecular Function | hydrolase activity | |
Molecular Function | lipid binding | |
Biological Process | signal transduction |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameProbable 2' cyclic ADP-D-ribose synthase TcpB
- EC number
- Short names2'cADPR synthase TcpB
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Brucellaceae > Brucella/Ochrobactrum group > Brucella
Accessions
- Primary accessionQ2YPC4
Proteomes
Subcellular Location
UniProt Annotation
GO Annotation
Note: Translocated into the host cell via the type IV secretion system (T4SS).
Keywords
- Cellular component
Phenotypes & Variants
Disruption phenotype
Grows as well as wild-type in mouse C57BL/6 dendritic cells, decreased activation of mouse dendritic cells compared to wild-type bacteria. Increased host expression and secretion of TNF-alpha and to a lesser extent IL-12 compared to wild-type bacteria.
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000458019 | 1-250 | Probable 2' cyclic ADP-D-ribose synthase TcpB | |||
Sequence: MSKEKQAQSKAHKAQQAISSAKSLSTQKSKMSELERATRDGAAIGKKRADIAKKIADKAKQLSSYQAKQFKADEQAVKKVAQEQKRLSDERTKHEAFIKQSLSSMRTTASATMEAEEEYDFFISHASEDKEAFVQDLAAALRDLGAKIFYDAYTLKVGDSLRRKIDQGLANSKFGIVVLSEHFFSKQWPARELDGLTAMEIGGQTRILPIWHKVSYDEVRRFSPSLADKVALNTSLKSVEEIAKELHSLI |
Interaction
Subunit
Homodimer. Interacts with host TIRAP. Interacts with host TLR4, abolishes the interaction of host TIRAP with TLR4.
Structure
Family & Domains
Features
Showing features for region, compositional bias, domain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-46 | Disordered | ||||
Sequence: MSKEKQAQSKAHKAQQAISSAKSLSTQKSKMSELERATRDGAAIGK | ||||||
Compositional bias | 11-31 | Polar residues | ||||
Sequence: AHKAQQAISSAKSLSTQKSKM | ||||||
Compositional bias | 32-46 | Basic and acidic residues | ||||
Sequence: SELERATRDGAAIGK | ||||||
Domain | 117-250 | TIR | ||||
Sequence: EEYDFFISHASEDKEAFVQDLAAALRDLGAKIFYDAYTLKVGDSLRRKIDQGLANSKFGIVVLSEHFFSKQWPARELDGLTAMEIGGQTRILPIWHKVSYDEVRRFSPSLADKVALNTSLKSVEEIAKELHSLI |
Domain
The TIR domain mediates NAD+ hydrolase (NADase) activity. The N-terminal region is required for localization to the host cell membrane.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length250
- Mass (Da)27,971
- Last updated2006-02-07 v1
- ChecksumE4188CE50BFD0C9D
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 11-31 | Polar residues | ||||
Sequence: AHKAQQAISSAKSLSTQKSKM | ||||||
Compositional bias | 32-46 | Basic and acidic residues | ||||
Sequence: SELERATRDGAAIGK |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
AM040264 EMBL· GenBank· DDBJ | CAJ10235.1 EMBL· GenBank· DDBJ | Genomic DNA |