Q2YM22 · CARA_BRUA2

Function

function

Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.

Catalytic activity

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.
Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site60L-glutamine (UniProtKB | ChEBI)
Binding site257L-glutamine (UniProtKB | ChEBI)
Binding site259L-glutamine (UniProtKB | ChEBI)
Active site286Nucleophile
Binding site287L-glutamine (UniProtKB | ChEBI)
Binding site290L-glutamine (UniProtKB | ChEBI)
Binding site328L-glutamine (UniProtKB | ChEBI)
Binding site330L-glutamine (UniProtKB | ChEBI)
Binding site331L-glutamine (UniProtKB | ChEBI)
Active site370
Active site372

GO annotations

AspectTerm
Molecular FunctionATP binding
Molecular Functioncarbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
Molecular Functionglutaminase activity
Biological Process'de novo' pyrimidine nucleobase biosynthetic process
Biological Process'de novo' UMP biosynthetic process
Biological Processarginine biosynthetic process
Biological Processglutamine metabolic process

Keywords

Enzyme and pathway databases

Protein family/group databases

Names & Taxonomy

Protein names

  • Recommended name
    Carbamoyl phosphate synthase small chain
  • EC number
  • Alternative names
    • Carbamoyl phosphate synthetase glutamine chain

Gene names

    • Name
      carA
    • Ordered locus names
      BAB1_1502

Organism names

  • Taxonomic identifier
  • Strain
    • 2308
  • Taxonomic lineage
    Bacteria > Pseudomonadota > Alphaproteobacteria > Hyphomicrobiales > Brucellaceae > Brucella/Ochrobactrum group > Brucella

Accessions

  • Primary accession
    Q2YM22

Proteomes

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_10001388531-407Carbamoyl phosphate synthase small chain

Interaction

Subunit

Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region, domain.

TypeIDPosition(s)Description
Region1-205CPSase
Domain209-397Glutamine amidotransferase type-1

Sequence similarities

Belongs to the CarA family.

Keywords

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    407
  • Mass (Da)
    43,536
  • Last updated
    2006-02-07 v1
  • Checksum
    D6C01AFF0592F7E2
MTETTPKTAPWTVQKRTAVLVLADGTVIEGKGLGATGAVEAEVVFNTALTGYEEILTDPSYAGQIVTFTFPHIGNVGANAEDIEDLTPANRHGAVGAIFKADITAPSNFRAAKDLDSWLKHRGIIALAGIDTRALTALIRERGAQNAVIAHDPNGNFDLDALKARAANWCGLENLDLAKDVTIGQSLVWKELPWTLQDGYGEQDAPQYHVVALDFGVKRNILRLLTGLGAKVTVLPATATAEDVLAHNPDGVFLSNGPGDPAATGEYAVPTIGKLVETGIPLFGICLGHQMLALALGGRTEKMHQGHHGANHPVKDYTTGKVEIVSMNHGFAVDSDSLPENVEETHVSLFDGTNCGLRVVGKPVFSVQHHPEASPGPQDSHYLFRRFINLIRERKGQAPLPEREQAA

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
AM040264
EMBL· GenBank· DDBJ
CAJ11458.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

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