Q2YDU8 · SPNS1_RAT

  • Protein
    Protein spinster homolog 1
  • Gene
    Spns1
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at transcript level
  • Annotation score
    5/5

Function

function

Plays a critical role in the phospholipid salvage pathway from lysosomes to the cytosol. Mediates the rate-limiting, proton-dependent, lysosomal efflux of lysophospholipids, which can then be reacylated by acyltransferases in the endoplasmic reticulum to form phospholipids. Selective for zwitterionic headgroups such as lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE), can also transport lysophosphatidylglycerol (LPG), but not other anionic lysophospholipids, sphingosine, nor sphingomyelin. Transports lysophospholipids with saturated, monounsaturated, and polyunsaturated fatty acids, such as 1-hexadecanoyl-sn-glycero-3-phosphocholine, 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine and 1-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine, respectively. Can also transport lysoplasmalogen (LPC with a fatty alcohol) such as 1-(1Z-hexadecenyl)-sn-glycero-3-phosphocholine. Essential player in lysosomal homeostasis. Crucial for cell survival under conditions of nutrient limitation. May be involved in necrotic or autophagic cell death.

Catalytic activity

  • a 1-acyl-sn-glycero-3-phosphocholine(out) + H+(out) = a 1-acyl-sn-glycero-3-phosphocholine(in) + H+(in)
  • 1-hexadecanoyl-sn-glycero-3-phosphocholine(out) + H+(out) = 1-hexadecanoyl-sn-glycero-3-phosphocholine(in) + H+(in)
  • 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine(out) + H+(out) = 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine(in) + H+(in)
  • 1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine(out) + H+(out) = 1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine(in) + H+(in)
  • 1-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine(out) + H+(out) = 1-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine(in) + H+(in)
  • a 1-acyl-sn-glycero-3-phosphoethanolamine(out) + H+(out) = a 1-acyl-sn-glycero-3-phosphoethanolamine(in) + H+(in)
  • 1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine(out) + H+(out) = 1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine(in) + H+(in)
  • 1-acyl-sn-glycero-3-phospho-(1'-sn-glycerol)(out) + H+(out) = 1-acyl-sn-glycero-3-phospho-(1'-sn-glycerol)(in) + H+(in)
  • 1-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol)(out) + H+(out) = 1-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol)(in) + H+(in)
  • 1-O-(1Z-alkenyl)-sn-glycero-3-phosphocholine(out) + H+(out) = 1-O-(1Z-alkenyl)-sn-glycero-3-phosphocholine(in) + H+(in)
  • 1-(1Z-hexadecenyl)-sn-glycero-3-phosphocholine(out) + H+(out) = 1-(1Z-hexadecenyl)-sn-glycero-3-phosphocholine(in) + H+(in)
  • 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine(out) + H+(out) = 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine(in) + H+(in)
  • 1-O-(1Z-hexadecenyl)-sn-glycero-3-phosphoethanolamine(out) + H+(out) = 1-O-(1Z-hexadecenyl)-sn-glycero-3-phosphoethanolamine(in) + H+(in)

GO annotations

AspectTerm
Cellular Componentlysosomal membrane
Cellular Componentlysosome
Cellular Componentmembrane
Molecular Functiontransmembrane transporter activity
Biological Processlysophospholipid transport
Biological Processphospholipid efflux
Biological Processregulation of lysosomal lumen pH

Keywords

Names & Taxonomy

Protein names

  • Recommended name
    Protein spinster homolog 1
  • Alternative names
    • RSpin1
    • Spns1

Gene names

    • Name
      Spns1

Organism names

  • Taxonomic identifier
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus

Accessions

  • Primary accession
    Q2YDU8

Proteomes

Organism-specific databases

Subcellular Location

Lysosome membrane
; Multi-pass membrane protein

Features

Showing features for transmembrane.

TypeIDPosition(s)Description
Transmembrane60-80Helical
Transmembrane98-118Helical
Transmembrane126-146Helical
Transmembrane160-180Helical
Transmembrane187-207Helical
Transmembrane218-238Helical
Transmembrane278-298Helical
Transmembrane323-343Helical
Transmembrane357-377Helical
Transmembrane381-401Helical
Transmembrane421-441Helical
Transmembrane465-485Helical

Keywords

PTM/Processing

Features

Showing features for initiator methionine, modified residue, chain.

TypeIDPosition(s)Description
Initiator methionine1Removed
Modified residue2N-acetylalanine
ChainPRO_00003639522-528Protein spinster homolog 1
Modified residue518Phosphoserine

Keywords

Proteomic databases

PTM databases

Expression

Gene expression databases

Interaction

Subunit

Interacts with BCL2 and BCL2L1.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for region.

TypeIDPosition(s)Description
Region1-38Disordered

Sequence similarities

Keywords

Phylogenomic databases

Family and domain databases

Sequence & Isoform

Align isoforms (2)
  • Sequence status
    Complete

This entry describes 2 isoforms produced by Alternative splicing.

Q2YDU8-1

This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

  • Length
    528
  • Mass (Da)
    56,796
  • Last updated
    2009-02-10 v2
  • Checksum
    5F098AFBD47C4AA5
MAGSDTAPFLSQADDPDDGPAPGHPGLPGPMGNPKSGELEVPDCEGLQRITGLSRGHSTLIVVVLCYINLLNYMDRFTVAGVLTDIEQFFNIGDGSTGLIQTVFISSYMVLAPVFGYLGDRYNRKYLMCGGIAFWSLVTLGSSFIPREHFWLLLLTRGLVGVGEASYSTIAPTLIADLFVADQRSRMLSIFYFAIPVGSGLGYIAGSKVKDLAGDWHWALRVTPGLGVLAVLLLFLVVQEPPRGAVERHSGSPPLSPTSWWADLKALARNPSFVLSSLGFTAVAFVTGSLALWAPAFLLRSRVVLGETPPCLPGDSCSSSDSLIFGLITCLTGVLGVGLGVEISRRLRRFNPRADPLVCAAGLLGSSPFLFLSLACARGSIVATYIFIFIGETLLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAGSPYLIGLISDRLRRSWPPSFLSEFRALQFSLMLCAFVGALGGAAFLGTAMFIENDRRRAQLHVQGLLHETEPSDDQIVVPQRGRSTRVPVSSVLI

Q2YDU8-2

  • Name
    2
  • See also
    sequence in UniParc or sequence clusters in UniRef
  • Differences from canonical
    • 322-528: SLIFGLITCLTGVLGVGLGVEISRRLRRFNPRADPLVCAAGLLGSSPFLFLSLACARGSIVATYIFIFIGETLLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAGSPYLIGLISDRLRRSWPPSFLSEFRALQFSLMLCAFVGALGGAAFLGTAMFIENDRRRAQLHVQGLLHETEPSDDQIVVPQRGRSTRVPVSSVLI → RYL

Computationally mapped potential isoform sequences

There are 5 potential isoforms mapped to this entry

View all
EntryEntry nameGene nameLength
A0A8I6ADI2A0A8I6ADI2_RATSpns1506
A0A8I6A590A0A8I6A590_RATSpns1165
A0A8I5ZQL7A0A8I5ZQL7_RATSpns1476
A0A8I5ZT04A0A8I5ZT04_RATSpns1584
A0A8I5ZSY7A0A8I5ZSY7_RATSpns1454

Sequence caution

The sequence BC087072 differs from that shown. Reason: Frameshift

Features

Showing features for alternative sequence.

TypeIDPosition(s)Description
Alternative sequenceVSP_036364322-528in isoform 2

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BC087072
EMBL· GenBank· DDBJ
-mRNA No translation available.
BC110048
EMBL· GenBank· DDBJ
AAI10049.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
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