Q2YDU8 · SPNS1_RAT
- ProteinProtein spinster homolog 1
- GeneSpns1
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids528 (go to sequence)
- Protein existenceEvidence at transcript level
- Annotation score5/5
Function
function
Plays a critical role in the phospholipid salvage pathway from lysosomes to the cytosol. Mediates the rate-limiting, proton-dependent, lysosomal efflux of lysophospholipids, which can then be reacylated by acyltransferases in the endoplasmic reticulum to form phospholipids. Selective for zwitterionic headgroups such as lysophosphatidylcholine (LPC) and lysophosphatidylethanolamine (LPE), can also transport lysophosphatidylglycerol (LPG), but not other anionic lysophospholipids, sphingosine, nor sphingomyelin. Transports lysophospholipids with saturated, monounsaturated, and polyunsaturated fatty acids, such as 1-hexadecanoyl-sn-glycero-3-phosphocholine, 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine and 1-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine, respectively. Can also transport lysoplasmalogen (LPC with a fatty alcohol) such as 1-(1Z-hexadecenyl)-sn-glycero-3-phosphocholine. Essential player in lysosomal homeostasis. Crucial for cell survival under conditions of nutrient limitation. May be involved in necrotic or autophagic cell death.
Catalytic activity
- a 1-acyl-sn-glycero-3-phosphocholine(out) + H+(out) = a 1-acyl-sn-glycero-3-phosphocholine(in) + H+(in)
- 1-hexadecanoyl-sn-glycero-3-phosphocholine(out) + H+(out) = 1-hexadecanoyl-sn-glycero-3-phosphocholine(in) + H+(in)
- 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine(out) + H+(out) = 1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine(in) + H+(in)
- 1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine(out) + H+(out) = 1-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphocholine(in) + H+(in)
- 1-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine(out) + H+(out) = 1-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholine(in) + H+(in)
- a 1-acyl-sn-glycero-3-phosphoethanolamine(out) + H+(out) = a 1-acyl-sn-glycero-3-phosphoethanolamine(in) + H+(in)
- 1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine(out) + H+(out) = 1-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine(in) + H+(in)
- 1-acyl-sn-glycero-3-phospho-(1'-sn-glycerol)(out) + H+(out) = 1-acyl-sn-glycero-3-phospho-(1'-sn-glycerol)(in) + H+(in)
- 1-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol)(out) + H+(out) = 1-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol)(in) + H+(in)
- 1-O-(1Z-alkenyl)-sn-glycero-3-phosphocholine(out) + H+(out) = 1-O-(1Z-alkenyl)-sn-glycero-3-phosphocholine(in) + H+(in)
- 1-(1Z-hexadecenyl)-sn-glycero-3-phosphocholine(out) + H+(out) = 1-(1Z-hexadecenyl)-sn-glycero-3-phosphocholine(in) + H+(in)
- 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine(out) + H+(out) = 1-O-(1Z-alkenyl)-sn-glycero-3-phosphoethanolamine(in) + H+(in)
- 1-O-(1Z-hexadecenyl)-sn-glycero-3-phosphoethanolamine(out) + H+(out) = 1-O-(1Z-hexadecenyl)-sn-glycero-3-phosphoethanolamine(in) + H+(in)
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | lysosomal membrane | |
Cellular Component | lysosome | |
Cellular Component | membrane | |
Molecular Function | transmembrane transporter activity | |
Biological Process | lysophospholipid transport | |
Biological Process | phospholipid efflux | |
Biological Process | regulation of lysosomal lumen pH |
Keywords
- Biological process
Names & Taxonomy
Protein names
- Recommended nameProtein spinster homolog 1
- Alternative names
Gene names
Organism names
- Organism
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Euarchontoglires > Glires > Rodentia > Myomorpha > Muroidea > Muridae > Murinae > Rattus
Accessions
- Primary accessionQ2YDU8
Proteomes
Organism-specific databases
Subcellular Location
UniProt Annotation
GO Annotation
Lysosome membrane ; Multi-pass membrane protein
Features
Showing features for transmembrane.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Transmembrane | 60-80 | Helical | ||||
Sequence: LIVVVLCYINLLNYMDRFTVA | ||||||
Transmembrane | 98-118 | Helical | ||||
Sequence: GLIQTVFISSYMVLAPVFGYL | ||||||
Transmembrane | 126-146 | Helical | ||||
Sequence: YLMCGGIAFWSLVTLGSSFIP | ||||||
Transmembrane | 160-180 | Helical | ||||
Sequence: VGVGEASYSTIAPTLIADLFV | ||||||
Transmembrane | 187-207 | Helical | ||||
Sequence: MLSIFYFAIPVGSGLGYIAGS | ||||||
Transmembrane | 218-238 | Helical | ||||
Sequence: WALRVTPGLGVLAVLLLFLVV | ||||||
Transmembrane | 278-298 | Helical | ||||
Sequence: LGFTAVAFVTGSLALWAPAFL | ||||||
Transmembrane | 323-343 | Helical | ||||
Sequence: LIFGLITCLTGVLGVGLGVEI | ||||||
Transmembrane | 357-377 | Helical | ||||
Sequence: LVCAAGLLGSSPFLFLSLACA | ||||||
Transmembrane | 381-401 | Helical | ||||
Sequence: IVATYIFIFIGETLLSMNWAI | ||||||
Transmembrane | 421-441 | Helical | ||||
Sequence: FQIVLSHLLGDAGSPYLIGLI | ||||||
Transmembrane | 465-485 | Helical | ||||
Sequence: MLCAFVGALGGAAFLGTAMFI |
Keywords
- Cellular component
PTM/Processing
Features
Showing features for initiator methionine, modified residue, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Initiator methionine | 1 | Removed | ||||
Sequence: M | ||||||
Modified residue | 2 | N-acetylalanine | ||||
Sequence: A | ||||||
Chain | PRO_0000363952 | 2-528 | Protein spinster homolog 1 | |||
Sequence: AGSDTAPFLSQADDPDDGPAPGHPGLPGPMGNPKSGELEVPDCEGLQRITGLSRGHSTLIVVVLCYINLLNYMDRFTVAGVLTDIEQFFNIGDGSTGLIQTVFISSYMVLAPVFGYLGDRYNRKYLMCGGIAFWSLVTLGSSFIPREHFWLLLLTRGLVGVGEASYSTIAPTLIADLFVADQRSRMLSIFYFAIPVGSGLGYIAGSKVKDLAGDWHWALRVTPGLGVLAVLLLFLVVQEPPRGAVERHSGSPPLSPTSWWADLKALARNPSFVLSSLGFTAVAFVTGSLALWAPAFLLRSRVVLGETPPCLPGDSCSSSDSLIFGLITCLTGVLGVGLGVEISRRLRRFNPRADPLVCAAGLLGSSPFLFLSLACARGSIVATYIFIFIGETLLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAGSPYLIGLISDRLRRSWPPSFLSEFRALQFSLMLCAFVGALGGAAFLGTAMFIENDRRRAQLHVQGLLHETEPSDDQIVVPQRGRSTRVPVSSVLI | ||||||
Modified residue | 518 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
PTM databases
Expression
Gene expression databases
Interaction
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 1-38 | Disordered | ||||
Sequence: MAGSDTAPFLSQADDPDDGPAPGHPGLPGPMGNPKSGE |
Sequence similarities
Belongs to the major facilitator superfamily. Spinster (TC 2.A.1.49) family.
Keywords
- Domain
Phylogenomic databases
Family and domain databases
Sequence & Isoform
- Sequence statusComplete
This entry describes 2 isoforms produced by Alternative splicing.
Q2YDU8-1
This isoform has been chosen as the canonical sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
- Name1
- Length528
- Mass (Da)56,796
- Last updated2009-02-10 v2
- Checksum5F098AFBD47C4AA5
Q2YDU8-2
- Name2
- Differences from canonical
- 322-528: SLIFGLITCLTGVLGVGLGVEISRRLRRFNPRADPLVCAAGLLGSSPFLFLSLACARGSIVATYIFIFIGETLLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAGSPYLIGLISDRLRRSWPPSFLSEFRALQFSLMLCAFVGALGGAAFLGTAMFIENDRRRAQLHVQGLLHETEPSDDQIVVPQRGRSTRVPVSSVLI → RYL
Computationally mapped potential isoform sequences
There are 5 potential isoforms mapped to this entry
Entry | Entry name | Gene name | Length | ||
---|---|---|---|---|---|
A0A8I6ADI2 | A0A8I6ADI2_RAT | Spns1 | 506 | ||
A0A8I6A590 | A0A8I6A590_RAT | Spns1 | 165 | ||
A0A8I5ZQL7 | A0A8I5ZQL7_RAT | Spns1 | 476 | ||
A0A8I5ZT04 | A0A8I5ZT04_RAT | Spns1 | 584 | ||
A0A8I5ZSY7 | A0A8I5ZSY7_RAT | Spns1 | 454 |
Sequence caution
Features
Showing features for alternative sequence.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Alternative sequence | VSP_036364 | 322-528 | in isoform 2 | |||
Sequence: SLIFGLITCLTGVLGVGLGVEISRRLRRFNPRADPLVCAAGLLGSSPFLFLSLACARGSIVATYIFIFIGETLLSMNWAIVADILLYVVIPTRRSTAEAFQIVLSHLLGDAGSPYLIGLISDRLRRSWPPSFLSEFRALQFSLMLCAFVGALGGAAFLGTAMFIENDRRRAQLHVQGLLHETEPSDDQIVVPQRGRSTRVPVSSVLI → RYL |
Keywords
- Coding sequence diversity
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BC087072 EMBL· GenBank· DDBJ | - | mRNA | No translation available. | |
BC110048 EMBL· GenBank· DDBJ | AAI10049.1 EMBL· GenBank· DDBJ | mRNA |