Q2XSC6 · LALIM_LAVAN
- Protein(R)-limonene synthase
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids602 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score3/5
Function
function
Catalyzes the formation of (R)-+-limonene, terpinolene, (1R,5S)-+-camphene, (1R,5R)-+-alpha-pinene, beta-myrcene and traces of alpha-phellandrene.
Catalytic activity
- (2E)-geranyl diphosphate = (4R)-limonene + diphosphate
Cofactor
Mn2+ (UniProtKB | Rhea| CHEBI:29035 )
Note: Binds 3 Mg2+ or Mn2+ ions per subunit.
Kinetics
KM | SUBSTRATE | pH | TEMPERATURE[C] | NOTES | EVIDENCE | |
---|---|---|---|---|---|---|
47.4 μM | geranyl diphosphate |
kcat is 0.012 sec-1 with geranyl diphosphate as substrate.
pH Dependence
Optimum pH is 7.0.
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 356 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 356 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 360 | Mg2+ 1 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 360 | Mg2+ 2 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 500 | Mg2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: D | ||||||
Binding site | 504 | Mg2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: T | ||||||
Binding site | 508 | Mg2+ 3 (UniProtKB | ChEBI) | ||||
Sequence: E |
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | (R)-limonene synthase activity | |
Molecular Function | magnesium ion binding | |
Molecular Function | pinene synthase activity | |
Biological Process | alpha-pinene biosynthetic process | |
Biological Process | diterpenoid biosynthetic process | |
Biological Process | green leaf volatile biosynthetic process | |
Biological Process | monoterpenoid biosynthetic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name(R)-limonene synthase
- EC number
- Short namesLaLIMS
Organism names
- Taxonomic lineageEukaryota > Viridiplantae > Streptophyta > Embryophyta > Tracheophyta > Spermatophyta > Magnoliopsida > eudicotyledons > Gunneridae > Pentapetalae > asterids > lamiids > Lamiales > Lamiaceae > Nepetoideae > Ocimeae > Lavandulinae > Lavandula
Accessions
- Primary accessionQ2XSC6
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000418951 | 1-602 | (R)-limonene synthase | |||
Sequence: MSIISMHVGILNRPAAYNHLRNLDRRASKPRHVSSTAAATRLRVSCATQLEIKSVDETRRSGNYNPTAWDFNYIQSLDNQYKKERYSTRHAELTVQVKKLLEEEMEAVQKLELIEDLKNLGISYPFKDNIQQILNQIYNEHKCCHNSEVEEKDLYFTALRFRLLRQQGFEVSQEVFDHFKNEKGTDFKPNLADDTKGLLQLYEASFLLREAEDTLELARQFSTKLLQKKVDENGDDKIEDNLLLWIRRSLELPLHWRVQRLEARGFLDAYVRRPDMNPIVFELAKLDFNITQATQQEELKDLSRWWNSTGLAEKLPFARDRVVESYFWAMGTFEPHQYGYQRELVAKIIALATVVDDVYDVYGTLEELELFTDAIRRWDRESIDQLPYYMQLCFLTVNNFVFELAHDVLKDKSFNCLPHLQRSWLDLAEAYLVEAKWYHSRYTPSLEEYLNIARVSVTCPTIVSQMYFALPIPIEKPVIEIMYKYHDILYLSGMLLRLPDDLGTASFELKRGDVQKAVQCYMKERNVPENEAREHVKFLIREASKQINTAMATDCPFTEDFAVAAANLGRVANFVYVDGDGFGVQHSKIYEQIGTLMFEPYP |
Structure
Sequence
- Sequence statusComplete
- Length602
- Mass (Da)70,345
- Last updated2005-12-20 v1
- Checksum1341E643455F34F2