Q2U0J7 · HEPG_ASPOR

Function

function

Glyceraldehyde-3-phosphate dehydrogenase; part of the gene cluster that mediates the biosynthesis of heptelidic acid (HA), a sesquiterpene lactone that acts as an inhibitor of glyceraldehyde-3-phosphatedehydrogenase (GAPDH) and a growth inhibitor of the salt-tolerant lactic acid bacteria in soy sauce brewing (PubMed:30466366).
The GAPDPH hepG/gdpB shows much higher resistance to HA than the GAPDH gpdA located outside of the cluster, but it does not seem to act in self-resistance (PubMed:30466366).

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
0.28 mMglyceraldehyde-3-phosphate (GAP)

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.

Features

Showing features for binding site, active site, site.

TypeIDPosition(s)Description
Binding site12-13NAD+ (UniProtKB | ChEBI)
Binding site34NAD+ (UniProtKB | ChEBI)
Binding site121NAD+ (UniProtKB | ChEBI)
Binding site149-151D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI)
Active site150Nucleophile
Site177Activates thiol group during catalysis
Binding site180D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI)
Binding site209-210D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI)
Binding site232D-glyceraldehyde 3-phosphate (UniProtKB | ChEBI)
Binding site314NAD+ (UniProtKB | ChEBI)

GO annotations

AspectTerm
Molecular Functionglyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
Molecular FunctionNAD binding
Molecular FunctionNADP binding
Biological Processglucose metabolic process
Biological Processglycolytic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Glyceraldehyde-3-phosphate dehydrogenase B
  • EC number
  • Short names
    GAPDH
  • Alternative names
    • Heptelidic acid biosynthesis cluster protein G

Gene names

    • Name
      hepG
    • Synonyms
      gpdB
    • ORF names
      AO090011000414

Organism names

Accessions

  • Primary accession
    Q2U0J7

Proteomes

Organism-specific databases

Phenotypes & Variants

Disruption phenotype

Does not affect the biosynthesis of heptelidic acid.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004508341-334Glyceraldehyde-3-phosphate dehydrogenase B

Interaction

Subunit

Homotetramer.

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    334
  • Mass (Da)
    36,080
  • Last updated
    2006-01-24 v1
  • Checksum
    D0618E15F55AA6CF
MTAKVGINGFGRIGRIVFRNSFSHENTEVVMVNDPFIEVQYAAYMLKYDSTHGNFEYDVHIDGDSIVVNGKKVKFYAEKDPAKIPWKDAGAEYIIESTGVFTTVEKASAHLQGGAKKVIISAPSADAPMYVMGVNEKTYAGADVVSNASCTTNCLAPLTKVLHERFGVVEGLMTAVHAYTATQKLVDAPSKKDWRGGRAAAQNLIPSSTGAAKAVGKVIPELQGKVTGMSIRVPTSNVSVVDLTCRLEKGASYEEIITAIKEAAQGELKGILDYTEDDVVSSDMKGNPHSSIVDIKAGISLNPNFLKIVSWYDNEWGYSRRVLDLTAYIASVGK

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BA000055
EMBL· GenBank· DDBJ
BAE64918.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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