Q2TBT5 · RNH2A_BOVIN

  • Protein
    Ribonuclease H2 subunit A
  • Gene
    RNASEH2A
  • Status
    UniProtKB reviewed (Swiss-Prot)
  • Amino acids
  • Protein existence
    Evidence at protein level
  • Annotation score
    4/5

Function

function

Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.

Catalytic activity

Cofactor

Mn2+ (UniProtKB | Rhea| CHEBI:29035 )

Mg2+ (UniProtKB | Rhea| CHEBI:18420 )

Note: Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.

Features

Showing features for binding site.

129920406080100120140160180200220240260280
TypeIDPosition(s)Description
Binding site34a divalent metal cation (UniProtKB | ChEBI)
Binding site35a divalent metal cation (UniProtKB | ChEBI)
Binding site142a divalent metal cation (UniProtKB | ChEBI)

GO annotations

AspectTerm
Cellular Componentcytosol
Cellular Componentnucleoplasm
Cellular Componentribonuclease H2 complex
Molecular Functionmetal ion binding
Molecular FunctionRNA binding
Molecular FunctionRNA-DNA hybrid ribonuclease activity
Biological ProcessDNA replication, removal of RNA primer
Biological Processmismatch repair
Biological ProcessRNA catabolic process

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Ribonuclease H2 subunit A
  • EC number
  • Short names
    RNase H2 subunit A
  • Alternative names
    • Ribonuclease HI large subunit (RNase HI large subunit)
    • Ribonuclease HI subunit A

Gene names

    • Name
      RNASEH2A

Organism names

  • Taxonomic identifier
  • Strain
    • Crossbred X Angus
  • Taxonomic lineage
    Eukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Bovinae > Bos

Accessions

  • Primary accession
    Q2TBT5

Proteomes

Organism-specific databases

Subcellular Location

Keywords

PTM/Processing

Features

Showing features for modified residue, chain.

TypeIDPosition(s)Description
Modified residue1N-acetylmethionine
ChainPRO_00002473211-299Ribonuclease H2 subunit A
Modified residue205Phosphothreonine
Modified residue217Phosphothreonine
Modified residue258Phosphoserine

Keywords

Proteomic databases

Expression

Gene expression databases

Interaction

Subunit

The RNase H2 complex is a heterotrimer composed of the catalytic subunit RNASEH2A and the non-catalytic subunits RNASEH2B and RNASEH2C.

Protein-protein interaction databases

Structure

Family & Domains

Features

Showing features for domain, compositional bias, region.

TypeIDPosition(s)Description
Domain28-251RNase H type-2
Compositional bias250-264Basic and acidic residues
Region250-272Disordered

Sequence similarities

Belongs to the RNase HII family. Eukaryotic subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    299
  • Mass (Da)
    33,300
  • Last updated
    2006-01-24 v1
  • Checksum
    902422B95D793608
MDLSELERDNTGRCRLSSPVPPVCLKEPCVLGVDEAGRGPVLGPMVYAICYCPLSRLEDLEALKVADSKTLSESERDRLFAKMEEDGDFVGWALDVLSPNLISTSMLGRVKYNLNALSHDTATGLVQFALDQGVNVAQVFVDTVGLPETYQERLQQRFPGIEVTVKAKADALYPVVSAASICAKVARDQAVKNWKFVEKLQDLDTDYGSGYPNDPKTKAWLRKHVDPVFGFPQFVRFSWRTAQSILESEAEDVKWEDSETGDPKGPGKIKSYFSESPQTCLRLPHRYFQERGLESATVL

Features

Showing features for compositional bias.

TypeIDPosition(s)Description
Compositional bias250-264Basic and acidic residues

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
BC109682
EMBL· GenBank· DDBJ
AAI09683.1
EMBL· GenBank· DDBJ
mRNA

Genome annotation databases

Similar Proteins

Disclaimer

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