Q2TBT5 · RNH2A_BOVIN
- ProteinRibonuclease H2 subunit A
- GeneRNASEH2A
- StatusUniProtKB reviewed (Swiss-Prot)
- Organism
- Amino acids299 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalytic subunit of RNase HII, an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Participates in DNA replication, possibly by mediating the removal of lagging-strand Okazaki fragment RNA primers during DNA replication. Mediates the excision of single ribonucleotides from DNA:RNA duplexes.
Catalytic activity
Cofactor
Mg2+ (UniProtKB | Rhea| CHEBI:18420 )
Note: Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.
Features
Showing features for binding site.
GO annotations
Aspect | Term | |
---|---|---|
Cellular Component | cytosol | |
Cellular Component | nucleoplasm | |
Cellular Component | ribonuclease H2 complex | |
Molecular Function | metal ion binding | |
Molecular Function | RNA binding | |
Molecular Function | RNA-DNA hybrid ribonuclease activity | |
Biological Process | DNA replication, removal of RNA primer | |
Biological Process | mismatch repair | |
Biological Process | RNA catabolic process |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameRibonuclease H2 subunit A
- EC number
- Short namesRNase H2 subunit A
- Alternative names
Gene names
Organism names
- Organism
- Strain
- Taxonomic lineageEukaryota > Metazoa > Chordata > Craniata > Vertebrata > Euteleostomi > Mammalia > Eutheria > Laurasiatheria > Artiodactyla > Ruminantia > Pecora > Bovidae > Bovinae > Bos
Accessions
- Primary accessionQ2TBT5
Proteomes
Organism-specific databases
Subcellular Location
PTM/Processing
Features
Showing features for modified residue, chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Modified residue | 1 | N-acetylmethionine | ||||
Sequence: M | ||||||
Chain | PRO_0000247321 | 1-299 | Ribonuclease H2 subunit A | |||
Sequence: MDLSELERDNTGRCRLSSPVPPVCLKEPCVLGVDEAGRGPVLGPMVYAICYCPLSRLEDLEALKVADSKTLSESERDRLFAKMEEDGDFVGWALDVLSPNLISTSMLGRVKYNLNALSHDTATGLVQFALDQGVNVAQVFVDTVGLPETYQERLQQRFPGIEVTVKAKADALYPVVSAASICAKVARDQAVKNWKFVEKLQDLDTDYGSGYPNDPKTKAWLRKHVDPVFGFPQFVRFSWRTAQSILESEAEDVKWEDSETGDPKGPGKIKSYFSESPQTCLRLPHRYFQERGLESATVL | ||||||
Modified residue | 205 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 217 | Phosphothreonine | ||||
Sequence: T | ||||||
Modified residue | 258 | Phosphoserine | ||||
Sequence: S |
Keywords
- PTM
Proteomic databases
Expression
Gene expression databases
Interaction
Subunit
The RNase H2 complex is a heterotrimer composed of the catalytic subunit RNASEH2A and the non-catalytic subunits RNASEH2B and RNASEH2C.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for domain, compositional bias, region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Domain | 28-251 | RNase H type-2 | ||||
Sequence: PCVLGVDEAGRGPVLGPMVYAICYCPLSRLEDLEALKVADSKTLSESERDRLFAKMEEDGDFVGWALDVLSPNLISTSMLGRVKYNLNALSHDTATGLVQFALDQGVNVAQVFVDTVGLPETYQERLQQRFPGIEVTVKAKADALYPVVSAASICAKVARDQAVKNWKFVEKLQDLDTDYGSGYPNDPKTKAWLRKHVDPVFGFPQFVRFSWRTAQSILESEAE | ||||||
Compositional bias | 250-264 | Basic and acidic residues | ||||
Sequence: AEDVKWEDSETGDPK | ||||||
Region | 250-272 | Disordered | ||||
Sequence: AEDVKWEDSETGDPKGPGKIKSY |
Sequence similarities
Belongs to the RNase HII family. Eukaryotic subfamily.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length299
- Mass (Da)33,300
- Last updated2006-01-24 v1
- Checksum902422B95D793608
Features
Showing features for compositional bias.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Compositional bias | 250-264 | Basic and acidic residues | ||||
Sequence: AEDVKWEDSETGDPK |
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
BC109682 EMBL· GenBank· DDBJ | AAI09683.1 EMBL· GenBank· DDBJ | mRNA |