Q2RXI0 · MTNA_RHORT

Function

function

Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P) (PubMed:23042035, PubMed:31950558).
Also catalyzes the interconversion of 5-deoxyribose 1-phosphate and 5-deoxyribulose 1-phosphate (PubMed:23042035, PubMed:31950558).
Part of a bifunctional DHAP-shunt salvage pathway for SAM by-products (PubMed:31950558).

Catalytic activity

Kinetics

KM SUBSTRATE pH TEMPERATURE[C] NOTES EVIDENCE
134 μMmethylthioribose-1-phosphate
171 μMdeoxyribose-1-phosphate
kcat is 0.1 sec-1 with methylthioribose-1-phosphate as substrate. kcat is 0.18 sec-1 with deoxyribose-1-phosphate as substrate (PubMed:23042035).
kcat is 0.03 sec-1 with ribose-1-phosphate as substrate (PubMed:31950558).

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 1/6.

Features

Showing features for binding site, site, active site.

TypeIDPosition(s)Description
Binding site53-55substrate
Binding site90substrate
Site168Transition state stabilizer
Binding site207substrate
Active site248Proton donor
Binding site258-259substrate

GO annotations

AspectTerm
Molecular FunctionS-methyl-5-thioribose-1-phosphate isomerase activity
Biological ProcessL-methionine salvage from methylthioadenosine
Biological ProcessL-methionine salvage from S-adenosylmethionine

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Methylthioribose-1-phosphate isomerase
  • EC number
  • Short names
    M1Pi
    ; MTR-1-P isomerase
  • Alternative names
    • S-methyl-5-thioribose-1-phosphate isomerase

Gene names

    • Name
      mtnA
    • Ordered locus names
      Rru_A0360

Organism names

Accessions

  • Primary accession
    Q2RXI0

Proteomes

Phenotypes & Variants

Disruption phenotype

Mutant cannot release methanethiol upon 5'-methylthioadenosine (MTA) feeding (PubMed:23042035).
Deletion mutant accumulates both S-methyl-5'-thioadenosine and 5'-deoxyadenosine extracellularly (PubMed:31950558).

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004454371-390Methylthioribose-1-phosphate isomerase

Interaction

Protein-protein interaction databases

Structure

Family & Domains

Sequence similarities

Belongs to the EIF-2B alpha/beta/delta subunits family. MtnA subfamily.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    390
  • Mass (Da)
    40,656
  • Last updated
    2006-01-24 v1
  • Checksum
    FAC7D0B6F8B90750
MNVKGTPTRTIWPAREGGAVWIIDQTRLPHEFVTQRLNDLGAVAHAIRAMLVRGAPLIGATAAYGVALGMAEDPSDEGLTRACQTLLATRPTAVNLRWAIEAMAESLAAVPPDQRAQAAWAKAGAICDEDVALNEAIGDHGLGIIKDLARTKGVEKGGEGPINILTHCNAGWLATVDWGTALAPLYKAHDAGLPIHVWVDETRPRNQGASLTAWELNSHGVPHTVIADNTGGHLMQHGLVDMVIVGTDRTTATGDVCNKIGTYLKALAAFDNAVPFYVALPGPTIDWTVNDGLREIPIEQRDAAEVTRVWGRTAAGALEWVTITPTGSPAANYAFDVTPARLITGLITERGVCAASAAGLAGLYPERAPAPVPAGSAAGKGAAATADGAL

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000230
EMBL· GenBank· DDBJ
ABC21165.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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