Q2RTX4 · Q2RTX4_RHORT
- ProteintRNA-dihydrouridine(20/20a) synthase
- GenedusA
- StatusUniProtKB unreviewed (TrEMBL)
- Amino acids349 (go to sequence)
- Protein existenceInferred from homology
- Annotation score3/5
Function
function
Catalyzes the synthesis of 5,6-dihydrouridine (D), a modified base found in the D-loop of most tRNAs, via the reduction of the C5-C6 double bond in target uridines. Specifically modifies U20 and U20a in tRNAs.
Catalytic activity
- 5,6-dihydrouridine20 in tRNA + NAD+ = uridine20 in tRNA + NADH + H+
- 5,6-dihydrouridine(20a) in tRNA + NAD+ = uridine(20a) in tRNA + NADH + H+
- 5,6-dihydrouridine(20a) in tRNA + NADP+ = uridine(20a) in tRNA + NADPH + H+
Cofactor
Features
Showing features for binding site, site, active site.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Binding site | 25-27 | FMN (UniProtKB | ChEBI) | |||
Binding site | 78 | FMN (UniProtKB | ChEBI) | |||
Site | 105 | Interacts with tRNA | |||
Active site | 108 | Proton donor | |||
Binding site | 147 | FMN (UniProtKB | ChEBI) | |||
Binding site | 179 | FMN (UniProtKB | ChEBI) | |||
Site | 191 | Interacts with tRNA; defines subfamily-specific binding signature | |||
Site | 194 | Interacts with tRNA | |||
Binding site | 219-221 | FMN (UniProtKB | ChEBI) | |||
Binding site | 248-249 | FMN (UniProtKB | ChEBI) | |||
Site | 314 | Interacts with tRNA; defines subfamily-specific binding signature | |||
Site | 317 | Interacts with tRNA; defines subfamily-specific binding signature | |||
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | flavin adenine dinucleotide binding | |
Molecular Function | FMN binding | |
Molecular Function | tRNA binding | |
Molecular Function | tRNA-dihydrouridine20 synthase activity | |
Molecular Function | tRNA-dihydrouridine20a synthase activity |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nametRNA-dihydrouridine(20/20a) synthase
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Rhodospirillales > Rhodospirillaceae > Rhodospirillum
Accessions
- Primary accessionQ2RTX4
Proteomes
Interaction
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region, domain.
Type | ID | Position(s) | Description | ||
---|---|---|---|---|---|
Region | 1-24 | Disordered | |||
Domain | 23-324 | DUS-like FMN-binding | |||
Sequence similarities
Belongs to the Dus family. DusA subfamily.
Belongs to the dus family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length349
- Mass (Da)37,800
- Last updated2006-01-24 v1
- Checksum8697330561D649F3
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000230 EMBL· GenBank· DDBJ | ABC22421.1 EMBL· GenBank· DDBJ | Genomic DNA |