Q2RSU5 · CUPIN_RHORT

Function

function

Catalyzes the formation of S-(methylsulfanyl)glutathione and 1-deoxy-D-xylulose 5-phosphate (DXP) from 1-methylthioxylulose 5-phosphate (MTXu-5P) (PubMed:23035785, PubMed:23042035).
The S-(methylsulfanyl)glutathione is reductively cleaved to relase methanethiol in a second reaction. Involved in the MTA-isoprenoid shunt of the methionine salvage pathway (PubMed:23042035).

Catalytic activity

Pathway

Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway.
Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site67Mn2+ (UniProtKB | ChEBI)
Binding site69Mn2+ (UniProtKB | ChEBI)
Binding site73Mn2+ (UniProtKB | ChEBI)
Binding site107Mn2+ (UniProtKB | ChEBI)
Active site121

GO annotations

AspectTerm
Molecular Functionlyase activity
Molecular Functionmetal ion binding
Molecular Functiontransferase activity
Biological Process1-deoxy-D-xylulose 5-phosphate biosynthetic process
Biological ProcessL-methionine salvage from methylthioadenosine

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    1-methylthio-D-xylulose 5-phosphate methylsulfurylase
  • EC number
  • Short names
    MTXu 5-P methylsulfurylase
  • Alternative names
    • 1-methylthio-xylulose 5-phosphate sulfo-lyase

Gene names

    • Name
      cupin
    • Ordered locus names
      Rru_A2000

Organism names

Accessions

  • Primary accession
    Q2RSU5

Proteomes

Phenotypes & Variants

Disruption phenotype

Mutant does not release methanethiol and does not show an increase in 1-deoxy-D-xylulose 5-phosphate formation after 5'-methylthioadenosine (MTA) feeding. Mutant accumulates a mixture of 1-methylthioxylulose 5-phosphate (MTXu-5P) and 1-methylthioribulose 5-phosphate (MTRu-5P) (PubMed:23042035).
Inactivation of the gene increases ethylene production from MTA-grown cells (PubMed:29133429).

Features

Showing features for mutagenesis.

TypeIDPosition(s)Description
Mutagenesis121Loss of activity.

PTM/Processing

Features

Showing features for chain.

TypeIDPosition(s)Description
ChainPRO_00004454361-1561-methylthio-D-xylulose 5-phosphate methylsulfurylase

Interaction

Protein-protein interaction databases

Family & Domains

Features

Showing features for domain.

TypeIDPosition(s)Description
Domain55-122Cupin type-2

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    156
  • Mass (Da)
    17,124
  • Last updated
    2006-01-24 v1
  • Checksum
    2685A30A37280CEE
MSDSNDDRPFRPFQSQYRWPGVDLLAYKEEGSAPFRSVTRQVLFSGNGLTGELRYFEVGPGGHSTLERHQHAHGVMILKGRGHAMVGRAVSAVAPYDLVTIPGWSWHQFRAPADEALGFLCMVNAERDKPQLPTEADLAMLRADDAVAAFLDGLAG

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000230
EMBL· GenBank· DDBJ
ABC22800.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

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