Q2RSU5 · CUPIN_RHORT
- Protein1-methylthio-D-xylulose 5-phosphate methylsulfurylase
- Genecupin
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids156 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score4/5
Function
function
Catalyzes the formation of S-(methylsulfanyl)glutathione and 1-deoxy-D-xylulose 5-phosphate (DXP) from 1-methylthioxylulose 5-phosphate (MTXu-5P) (PubMed:23035785, PubMed:23042035).
The S-(methylsulfanyl)glutathione is reductively cleaved to relase methanethiol in a second reaction. Involved in the MTA-isoprenoid shunt of the methionine salvage pathway (PubMed:23042035).
The S-(methylsulfanyl)glutathione is reductively cleaved to relase methanethiol in a second reaction. Involved in the MTA-isoprenoid shunt of the methionine salvage pathway (PubMed:23042035).
Catalytic activity
- glutathione + S-methyl-1-thio-D-xylulose 5-phosphate = 1-deoxy-D-xylulose 5-phosphate + S-(methylsulfanyl)glutathioneThis reaction proceeds in the forward direction.
- AH2 + S-(methylsulfanyl)glutathione = A + glutathione + methanethiolThis reaction proceeds in the forward direction.
Pathway
Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway.
Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis.
Features
Showing features for binding site, active site.
GO annotations
Aspect | Term | |
---|---|---|
Molecular Function | lyase activity | |
Molecular Function | metal ion binding | |
Molecular Function | transferase activity | |
Biological Process | 1-deoxy-D-xylulose 5-phosphate biosynthetic process | |
Biological Process | L-methionine salvage from methylthioadenosine |
Keywords
- Molecular function
- Biological process
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended name1-methylthio-D-xylulose 5-phosphate methylsulfurylase
- EC number
- Short namesMTXu 5-P methylsulfurylase
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Rhodospirillales > Rhodospirillaceae > Rhodospirillum
Accessions
- Primary accessionQ2RSU5
Proteomes
Phenotypes & Variants
Disruption phenotype
Mutant does not release methanethiol and does not show an increase in 1-deoxy-D-xylulose 5-phosphate formation after 5'-methylthioadenosine (MTA) feeding. Mutant accumulates a mixture of 1-methylthioxylulose 5-phosphate (MTXu-5P) and 1-methylthioribulose 5-phosphate (MTRu-5P) (PubMed:23042035).
Inactivation of the gene increases ethylene production from MTA-grown cells (PubMed:29133429).
Inactivation of the gene increases ethylene production from MTA-grown cells (PubMed:29133429).
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 121 | Loss of activity. | ||||
Sequence: C → A |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000445436 | 1-156 | 1-methylthio-D-xylulose 5-phosphate methylsulfurylase | |||
Sequence: MSDSNDDRPFRPFQSQYRWPGVDLLAYKEEGSAPFRSVTRQVLFSGNGLTGELRYFEVGPGGHSTLERHQHAHGVMILKGRGHAMVGRAVSAVAPYDLVTIPGWSWHQFRAPADEALGFLCMVNAERDKPQLPTEADLAMLRADDAVAAFLDGLAG |
Interaction
Protein-protein interaction databases
Structure
Sequence
- Sequence statusComplete
- Length156
- Mass (Da)17,124
- Last updated2006-01-24 v1
- Checksum2685A30A37280CEE
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
CP000230 EMBL· GenBank· DDBJ | ABC22800.1 EMBL· GenBank· DDBJ | Genomic DNA |