Q2RSB2 · PNTAA_RHORT
- ProteinNAD(P) transhydrogenase subunit alpha part 1
- GenepntAA
- StatusUniProtKB reviewed (Swiss-Prot)
- Amino acids384 (go to sequence)
- Protein existenceEvidence at protein level
- Annotation score5/5
Function
function
The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane.
Catalytic activity
- H+(in) + NAD+ + NADPH = H+(out) + NADH + NADP+
Features
Showing features for binding site.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Binding site | 127-129 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: RIS | ||||||
Binding site | 132-135 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: QSMD | ||||||
Binding site | 180-182 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: VGV | ||||||
Binding site | 202-204 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: DVR | ||||||
Binding site | 234 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: G | ||||||
Binding site | 247 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: Q | ||||||
Binding site | 266 | NAD+ (UniProtKB | ChEBI) | ||||
Sequence: L |
GO annotations
all annotations | all molecular function | virus receptor activity | dna binding | rna binding | cytoskeletal motor activity | catalytic activity | gtpase activity | structural molecule activity | transporter activity | cytoskeletal protein binding | lipid binding | cyclase activity | antioxidant activity | oxidoreductase activity | transferase activity | hydrolase activity | lyase activity | isomerase activity | ligase activity | protein tag activity | cargo receptor activity | histone binding | protein folding chaperone | translation regulator activity | nutrient reservoir activity | receptor ligand activity | molecular transducer activity | molecular adaptor activity | toxin activity | cell adhesion mediator activity | molecular function regulator activity | virus coreceptor activity | catalytic activity, acting on a protein | catalytic activity, acting on dna | catalytic activity, acting on rna | molecular carrier activity | transcription regulator activity | general transcription initiation factor activity | molecular sensor activity | molecular sequestering activity | atp-dependent activity | other molecular function | all biological process | mitotic cell cycle | cytokinesis | cytoplasmic translation | immune system process | muscle system process | circulatory system process | renal system process | respiratory system process | carbohydrate metabolic process | generation of precursor metabolites and energy | dna replication | dna repair | dna recombination | chromatin organization | dna-templated transcription | regulation of dna-templated transcription | trna metabolic process | protein folding | protein glycosylation | amino acid metabolic process | modified amino acid metabolic process | lipid metabolic process | vitamin metabolic process | sulfur compound metabolic process | intracellular protein transport | nucleocytoplasmic transport | autophagy | inflammatory response | mitochondrion organization | cytoskeleton organization | microtubule-based movement | peroxisome organization | lysosome organization | chromosome segregation | cell adhesion | establishment or maintenance of cell polarity | programmed cell death | photosynthesis | mrna metabolic process | snrna metabolic process | vesicle-mediated transport | reproductive process | digestive system process | signaling | cell differentiation | protein catabolic process | extracellular matrix organization | regulatory ncrna-mediated gene silencing | telomere organization | cell junction organization | wound healing | ribosome biogenesis | cilium organization | anatomical structure development | cell motility | nervous system process | endocrine process | protein maturation | transmembrane transport | nucleobase-containing small molecule metabolic process | hepaticobiliary system process | membrane organization | protein-containing complex assembly | cell wall organization or biogenesis | nitrogen cycle metabolic process | protein localization to plasma membrane | defense response to other organism | detoxification | meiotic nuclear division | mitotic nuclear division | mitochondrial gene expression | carbohydrate derivative metabolic process | other biological process | all cellular component | nuclear chromosome | extracellular region | extracellular space | cell wall | nucleus | nuclear envelope | nucleoplasm | chromosome | nucleolus | mitochondrion | lysosome | endosome | vacuole | peroxisome | endoplasmic reticulum | golgi apparatus | lipid droplet | microtubule organizing center | cytosol | ribosome | cytoskeleton | plasma membrane | cilium | plastid | thylakoid | external encapsulating structure | extracellular matrix | cytoplasmic vesicle | organelle | other cellular component | |||
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Aspect | Term | |
---|---|---|
Molecular Function | NAD binding | |
Molecular Function | NAD(P)+ transhydrogenase (Si-specific) activity | |
Molecular Function | NAD+ binding | |
Molecular Function | NADH binding | |
Molecular Function | protein dimerization activity | |
Molecular Function | proton-translocating NAD(P)+ transhydrogenase activity | |
Biological Process | NADPH regeneration |
Keywords
- Molecular function
- Ligand
Enzyme and pathway databases
Names & Taxonomy
Protein names
- Recommended nameNAD(P) transhydrogenase subunit alpha part 1
- EC number
- Alternative names
Gene names
Organism names
- Strain
- Taxonomic lineageBacteria > Pseudomonadota > Alphaproteobacteria > Rhodospirillales > Rhodospirillaceae > Rhodospirillum
Accessions
- Primary accessionQ2RSB2
- Secondary accessions
Proteomes
Phenotypes & Variants
Features
Showing features for mutagenesis.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Mutagenesis | 127 | No effect on interaction with PntB, but hydride transfer inhibited. Much weaker binding to NADH. | ||||
Sequence: R → A or M | ||||||
Mutagenesis | 132 | No effect on interaction with PntB, but hydride transfer strongly inhibited. No effect on NADH binding affinity on its own, but differences in NADH binding properties in complex with PntB. | ||||
Sequence: Q → N | ||||||
Mutagenesis | 135 | No effect on interaction with PntB, but hydride transfer strongly inhibited. No effect on the binding affinity to NADH. | ||||
Sequence: D → N | ||||||
Mutagenesis | 138 | No effect on interaction with PntB, but hydride transfer strongly inhibited. No effect on the binding affinity to NADH. | ||||
Sequence: S → A |
PTM/Processing
Features
Showing features for chain.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Chain | PRO_0000231663 | 1-384 | NAD(P) transhydrogenase subunit alpha part 1 | |||
Sequence: MKIAIPKERRPGEDRVAISPEVVKKLVGLGFEVIVEQGAGVGASITDDALTAAGATIASTAAQALSQADVVWKVQRPMTAEEGTDEVALIKEGAVLMCHLGALTNRPVVEALTKRKITAYAMELMPRISRAQSMDILSSQSNLAGYRAVIDGAYEFARAFPMMMTAAGTVPPARVLVFGVGVAGLQAIATAKRLGAVVMATDVRAATKEQVESLGGKFITVDDEAMKTAETAGGYAKEMGEEFRKKQAEAVLKELVKTDIAITTALIPGKPAPVLITEEMVTKMKPGSVIIDLAVEAGGNCPLSEPGKIVVKHGVKIVGHTNVPSRVAADASPLFAKNLLNFLTPHVDKDTKTLVMKLEDETVSGTCVTRDGAIVHPALTGQGA |
Interaction
Subunit
Heterotrimer of two alpha chains and a beta (PntB) chain; in Rhodospirillum, the alpha chain is made of two subunits (PntAA and PntAB) and forms a dimer.
Protein-protein interaction databases
Structure
Family & Domains
Features
Showing features for region.
Type | ID | Position(s) | Description | |||
---|---|---|---|---|---|---|
Region | 126-136 | RQD loop; involved in interaction with PntB | ||||
Sequence: PRISRAQSMDI |
Sequence similarities
Belongs to the AlaDH/PNT family.
Phylogenomic databases
Family and domain databases
Sequence
- Sequence statusComplete
- Length384
- Mass (Da)40,277
- Last updated2006-01-24 v1
- ChecksumB886A640CE2BFA12
Keywords
- Technical term
Sequence databases
Nucleotide Sequence | Protein Sequence | Molecule Type | Status | |
---|---|---|---|---|
U05294 EMBL· GenBank· DDBJ | AAA62493.1 EMBL· GenBank· DDBJ | Genomic DNA | ||
CP000230 EMBL· GenBank· DDBJ | ABC22983.1 EMBL· GenBank· DDBJ | Genomic DNA |