Q2RQK6 · Q2RQK6_RHORT

Function

function

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor, and NADPH and FADH2 as the reductant.

Catalytic activity

Cofactor

FAD (UniProtKB | Rhea| CHEBI:57692 )

Note: Binds 4 FAD per tetramer. Each FAD binding site is formed by three monomers.

Pathway

Pyrimidine metabolism; dTTP biosynthesis.

Features

Showing features for binding site, active site.

TypeIDPosition(s)Description
Binding site91FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits
Binding site112-115dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners
Binding site115-117FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits
Binding site124FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits
Binding site124-128dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site208dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners; in other chain
Binding site224-226FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits
Binding site230FAD (UniProtKB | ChEBI); ligand shared between neighboring subunits
Active site235Involved in ionization of N3 of dUMP, leading to its activation
Binding site235dUMP (UniProtKB | ChEBI); ligand shared between dimeric partners

GO annotations

AspectTerm
Molecular Functionflavin adenine dinucleotide binding
Molecular Functionthymidylate synthase (FAD) activity
Biological ProcessdTMP biosynthetic process
Biological ProcessdTTP biosynthetic process
Biological Processmethylation

Keywords

Enzyme and pathway databases

Names & Taxonomy

Protein names

  • Recommended name
    Flavin-dependent thymidylate synthase
  • EC number
  • Short names
    FDTS
  • Alternative names
    • FAD-dependent thymidylate synthase
    • Thymidylate synthase ThyX
      (TS
      ; TSase
      )

Gene names

    • Name
      thyX
    • Ordered locus names
      Rru_A2792

Organism names

Accessions

  • Primary accession
    Q2RQK6

Proteomes

Interaction

Subunit

Homotetramer.

Protein-protein interaction databases

Family & Domains

Sequence similarities

Belongs to the thymidylate synthase ThyX family.

Phylogenomic databases

Family and domain databases

Sequence

  • Sequence status
    Complete
  • Length
    285
  • Mass (Da)
    30,689
  • Last updated
    2006-01-24 v1
  • Checksum
    86B7AA39ADECA040
MTELLFAPTATVVAHSSMDPAGLAGWAAEMGYGALIDDATTPLGDIAQASTGAGSADVLPEFAGRFCYRSFKKGRPADEYVAHILDSGHGSVLEHVSVSFAVTGVSRALTHELIRHRAGTAVSQESQRYVDAKSVNFVVPPLLVAEIDRLKASGAAGEADALCAEFLTDCESALGRYKRWQESFRAQVEALGVSEPTLIKKRANEAARCILPNATETRLVWTMNLRSARHVVELRGHRDADLEIRRLSCLLARKMKEVAPLVFADVGVFTDSDGFESVGVGHHKV

Keywords

Sequence databases

Nucleotide SequenceProtein SequenceMolecule TypeStatus
CP000230
EMBL· GenBank· DDBJ
ABC23589.1
EMBL· GenBank· DDBJ
Genomic DNA

Genome annotation databases

Similar Proteins

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. Our staff consists of biologists and biochemists that are not trained to give medical advice.
We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.
FeedbackHelp